Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (1): 91-94.DOI: 10.11853/j.issn.1003.8280.2019.01.022

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Genotyping and sequence analysis of G2 segment of hantavirus carried by rats in Shenyang, China

CHE Lei, ZHANG Guo-bin, GAO Dan, ZHAO Liang, WANG Xiao-li   

  1. Shenyang Center for Disease Control and Prevention, Shenyang 110031, Liaoning Province, China
  • Received:2018-09-25 Online:2019-02-20 Published:2019-02-20


车雷, 张国斌, 高丹, 赵梁, 王晓丽   

  1. 沈阳市疾病预防控制中心自然疫源性疾病科, 辽宁 沈阳 110031
  • 作者简介:车雷,男,副主任医师,主要从事自然疫源性疾病防制研究,

Abstract: Objective To investigate the genotype and variation of hantavirus (HV) carried by host animals in the epidemic foci of hemorrhagic fever with renal syndrome (HFRS) in Shenyang, China. Methods The high-incidence areas of HFRS in Shenyang were selected. The host animals (rats) were captured simultaneously in residential areas and the field in the spring and autumn of 2017, and the rat lungs were collected. The HV antigen-positive rat lungs were detected by immunofluorescence assay (IFA), from which G2 segments were amplified by RT-PCR. Agarose gel electrophoresis was used to identify the genotype. Nucleotide sequencing of the amplified products was performed. Homology analysis was performed by DNAStar package. Results Seventeen HFRS-positive rat lung specimens were screened out by IFA. Gene segments of interest were amplified from 10 specimens by RT-PCR, all of which belonged to Seoul virus (SEO) type. The sequencing results of the amplified products indicated that, compared with domestic strains and some international standard strains, the nucleotide homology of the 10 SEO-type HV specimens was 99.3%-100%. The homology with the 80-39 strain (South Korea, 1996) was the highest (95.6%-96.2%). The deduced amino acid homology was 99.3%. Conclusion SEO was the major type of HV in HFRS epidemic foci in Shenyang. The G2 segment of HV carried by rats was highly homologous and had no obvious variation, and the genetic materials were relatively stable.

Key words: Hantavirus, Rat lung, G2 segment, Phylogenetic tree

摘要: 目的 了解沈阳市肾综合征出血热(HFRS)疫源地宿主动物携带汉坦病毒(HV)的基因型别及变异情况。方法 选取沈阳市HFRS高发地区,于2017年春、秋两季分别在居民区和野外同时捕获宿主动物(鼠),并采集鼠肺。采用间接免疫荧光试验(IFA)筛选出HV抗原阳性的鼠肺,用RT-PCR从IFA筛选阳性的鼠肺中扩增G2基因片段。经琼脂糖凝胶电泳进行基因分型,对扩增产物进行核苷酸序列测定,用DNAStar软件进行同源性分析。结果 筛选出HFRS抗原阳性的鼠肺17份,对阳性鼠肺标本经RT-PCR检测,10份标本扩增出目的片段,均为HV汉城型(SEO)。扩增产物测序结果与国内毒株及部分国际标准株比较,10份SEO型HV之间核苷酸同源性高达99.3%~100%。与80-39株(韩国,1980)同源性最高,为95.6%~96.2%,推导氨基酸同源性为99.3%。结论 沈阳市HFRS鼠间疫情的HV基因型以SEO型为主,检测出的鼠间HV G2基因片段之间高度同源,无明显变异,遗传物质相对稳定。

关键词: 汉坦病毒, 鼠肺, G2片段, 系统进化树

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