Chines Journal of Vector Biology and Control ›› 2015, Vol. 26 ›› Issue (4): 379-382.DOI: 10.11853/j.issn.1003.4692.2015.04.013

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Investigation and analysis on host animals and pathogen of hemorrhagic fever with renal syndrome in Xi'an city, Shaanxi, China

JIN Tie-zhi1,2, WU Rui3, CHEN Hai-long3, CHEN Xiao-ning1, MA Chao-feng3, WANG Kai-feng1   

  1. 1 Shaanxi Institute of Zoology, Xi'an 710032, Shaanxi Province, China;
    2 The College of Life Sciences, Northwest University, Xi'an 710069, Shaanxi Province, China;
    3 Xi'an Center for Disease Control and Prevention
  • Received:2015-02-27 Online:2015-08-20 Published:2015-08-20
  • Supported by:

    Supported by the Shaanxi National Science Foundation(No. 2012JM3013), Shaanxi Academy of Sciences Applying Basic Research Specific Funds(No. 2014K-08)and the Shaanxi Academy of Sciences(No. 2013K-28)

西安市肾综合征出血热宿主动物调查及病原分析

靳铁治1,2, 吴瑞3, 陈海龙3, 陈晓宁1, 马超锋3, 王开锋1   

  1. 1 陕西省动物研究所, 西安710032;
    2 西北大学生命科学学院, 陕西西安710069;
    3 西安市疾病预防控制中心
  • 通讯作者: 王开锋,Email: wangkf@ms.xab.cn
  • 作者简介:靳铁治,男,助理研究员,主要从事生物多样性及灾害生物防治工作,Email: 43155487@qq.com
  • 基金资助:

    陕西省自然科学基金(2012JM3013);陕西省科学院应用基础(2014K-08);陕西省科学院(2013K-28)

Abstract:

Objective To get an insight into the species composition, distribution and virus-carrying condition of host animals in Xi'an of hemorrhagic fever with renal syndrome (HFRS) in 2012. Methods Night trapping method was used to capture murine-like animals, and their habitats were recorded, and extracted RNA and genotyping of Hantavirus(HV) carried by the animals, the differences in rodent density, and virus-carrying rate between different months were analyzed by using t -Test, and the differences of rodent density in different habitats were analyzed by using general linear models. Results There were 480 murine-like animals were captured belonging to 12 species/subspecies of 6 genus, 4 families, and 2 orders in 2012, the density of animals in the field (9.42%) was higher than in the populated area (3.93%) (F=4.555, P < 0.05). There were significant difference in rodent density and virus-carrying rate among different months(t=4.900, P < 0.05; t=5.894, P < 0.05). Animal density began to significantly increase from May, and reached its peak in August, and then drop after November. The virus-carrying rates of animal were 28.53% in the field and 0 in the populated area. Three species were virus - carrying animal, which were Apodemus agrarius, Mus musculus and Crocidura suaveolens. All the genotypes of 119 positive rodents belonged to typeⅠ (hantaan virus, HTNV). Among these positive rodents, A. agrarius accounted for 97.48%, and its virus-carrying rate was 31.52%. Conclusion Apodemus agrarius is still the main host species of virus which causes HFRS in Xi'an. Controlling density of field murine-like animals should be the key to prevent HFRS, and taking comprehensive measures for controlling animal density should begin in April.

Key words: Hemorrhagic fever with renal syndrome, Host animal, Hantavirus

摘要:

目的 了解西安市肾综合征出血热(HFRS)宿主动物的构成、分布及携带病毒状况。方法 以夹夜法捕鼠,记录鼠种和生存环境,无菌解剖后取鼠肺提取RNA,扩增汉坦病毒M片段基因进行病毒分型。采用t 检验分析不同月份间鼠密度及鼠带病毒率是否存在差异,一般线性模型分析不同生境害鼠密度的差异。结果 2012年共捕获2目4科6属12种480只鼠形动物,居民区鼠形动物密度为3.93%,野外为9.42%,野外鼠形动物密度显著高于居民区(F=4.555,P < 0.05)。鼠形动物密度及带病毒率月份间差异有统计学意义(t=4.900,P < 0.05;t=5.894,P < 0.05),鼠形动物密度从5月显著上升,8月为最高,11月后开始明显下降。野外鼠形动物带病毒率为28.53%,居民区未检出带病毒鼠,带病毒鼠形动物种类有3种,分别为黑线姬鼠、小家鼠、北小麝鼩,其携带病毒均为Ⅰ型(汉滩型),黑线姬鼠占总带病毒鼠的97.48%,其带病毒率为31.52%。结论 黑线姬鼠仍是该地区HFRS最主要的宿主动物;野外鼠应为防控重点,建议对重点疫区鼠类从4月开始实施综合防控措施。

关键词: 肾综合征出血热, 宿主动物, 汉坦病毒

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