Abstract: Objective To observe the growth of Yersinia pestis on different kinds of culture media and screen for high quality culture media. Methods Make 5 kinds of culture media (Hiss Agar, Nutrient Agar, Sulfite Agar base, Hemolysis Agar Base, and Agar Hottinger nutrient)according to the instructions and pour into plates. Inoculating 0.1 ml Y. pestis suspensions on the prepared plate, calculating viable bacteria after 48-hour cultivation under 28℃ and observing the growth of Y. pestis on different culture media. Results There is no significant difference among Hiss Agar, Nutrient Agar and Sulfite Agar base. And the difference between Hemolysis Agar Base and Agar Hottinger nutrient was not significant either. The first three culture media and the following two culture media were also not significantly different. Hemolysis Agar Base and Agar Hottinger nutrient were superior to other culture media Hiss Agar, Nutrient Agar, Sulfite Agar base on growth of Y. pestis. Conclusion All the five culture media can be used for culture of Y. pestis, but Hemolysis Agar Base and Agar Hottinger nutrient were superior to the others.

Key words: Culture media, Yersinia pestis, Effect evaluation

摘要： 目的 观察不同干粉培养基对鼠疫耶尔森菌（鼠疫菌）的生长作用，筛选优质干粉培养基。方法 分别按照5种干粉培养基（赫氏培养基、营养琼脂、亚硫酸钠琼脂基础、溶血琼脂基础和赫金格尔干粉培养基）说明制作培养基，倾注平皿。将0.1 ml鼠疫菌悬液涂布于制备好的平皿中，于28℃ 48 h培养后进行活菌计数，观察鼠疫菌在5种干粉培养基中的生长效果。结果 鼠疫菌在赫氏培养基、营养琼脂、亚硫酸钠琼脂基础培养基中的生长情况相互间差异无统计学意义（P > 0.05）；在溶血琼脂基础、赫金格尔干粉培养基组间差异无统计学意义（P > 0.05）；鼠疫菌在溶血琼脂基础和赫金格尔干粉培养基中的生长效果优于赫氏培养基、营养琼脂和亚硫酸钠琼脂基础培养基，差异有统计学意义（P < 0.05）。结论 5种干粉培养基均可用于鼠疫菌的分离培养，但溶血琼脂基础干粉和赫金格尔干粉培养基中鼠疫菌的生长效果优于其他3种干粉培养基。

关键词: 干粉培养基, 鼠疫耶尔森菌, 效果评价