Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (6): 607-612.DOI: 10.11853/j.issn.1003.8280.2019.06.003

• Original Reports • Previous Articles     Next Articles

An analysis of bacterial community composition and diversity in Ixodes persulcatus in Inner Mongolia, China

LI Si-si1,2, ZHANG Xiao-yu1, ZHANG Yan-kai1, LIU Jing-ze1   

  1. 1 Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei Province, China;
    2 College of Life Sciences, Hengshui University
  • Received:2019-08-05 Online:2019-12-20 Published:2019-12-20
  • Supported by:
    Supported by the National Natural Science Foundation of China (No. 31802008)


李思思1,2, 张晓雨1, 张艳凯1, 刘敬泽1   

  1. 1 河北省动物生理生化与分子生物学重点实验室, 河北师范大学生命科学学院, 河北 石家庄 050024;
    2 衡水学院生命科学学院, 河北 衡水 053000
  • 通讯作者: 刘敬泽,
  • 作者简介:李思思,女,在读博士,讲师,主要从事蜱类生理生态学研究,
  • 基金资助:

Abstract: Objective To investigate the bacterial community composition and population diversity in Ixodes persulcatus. Methods Roche 454 high-throughput sequencing technology was applied to determine the highly variable V3 segment of 16S rDNA gene of I. persulcatus in Inner Mongolia. Meanwhile, analyses were performed on bacterial community composition, population diversity, dominant bacterial population, and distribution of common pathogens. Results A total of 22 727 sequences and 1 570 operational taxonomic units were obtained for analysis. The coverage of all samples was greater than or equal to 0.95, which indicated that the number of sequences for sequencing was sufficient for covering basically all the bacterial species. Among the samples, male ticks had the highest richness index and diversity index (2 431 and 4.11, respectively), followed by female ticks and eggs. The dominant bacteria in I. persulcatus were Proteobacteria, Firmicutes, and Actinobacteria, sequentially, among which Alphaproteobacteria showed the highest relative abundance. On the genus level, the composition of genera with higher relative abundance varied greatly between different types of samples. Bacteria found in the samples were pathogens (i.e., Candidatus Rickettsia tarasevichiae, Spiroplasma, and Borrelia miyamotoi), environmental bacteria (i.e., Chryseobacterium, Erwinia, and Brevundimonas), and maternally inherited symbiont (i.e., Montezuma). Conclusion There are relatively significant differences in bacterial community composition and abundance between different I. persulcatus samples in Inner Mongolia.

Key words: Ixodes persulcatus, Bacterial community composition, Diversity, 16S rDNA, Roche 454 high-throughput sequencing

摘要: 目的 了解全沟硬蜱细菌的群落结构及种群多样性。方法 应用罗氏454高通量测序技术对内蒙古地区全沟硬蜱细菌16S rDNA基因的V3高变异区段进行测定,分析其细菌的群落结构、种群多样性、优势菌群以及常见病原体的分布。结果 获得用于分析的序列和操作分类单元(OTUs)数目分别为22 727条和1 570个;所有样本的覆盖度均≥ 0.95,表明本次测序量充分,基本可覆盖所有细菌物种。其中,雄蜱样本的丰富度指数、多样性指数最高,分别为2 431和4.11;其次为雌蜱和卵。全沟硬蜱中优势菌群依次为变形菌门、厚壁菌门、放线菌门,其中以α-变形菌纲相对丰度最高;在属水平上,不同类别样本中相对丰度较高的菌属组成差异较大。在样本中检测到致病菌塔拉塞维奇立克次体、螺原体和宫本疏螺旋体,环境细菌如金黄杆菌属、欧文菌、短波单胞菌等,以及母系遗传共生菌(Montezuma)。结论 内蒙古地区全沟硬蜱不同样本间细菌群落和丰度具有较大差异。

关键词: 全沟硬蜱, 细菌群落结构, 多样性, 16S rDNA, 罗氏454高通量测序

CLC Number: