Investigation of Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China

doi:10.11853/j.issn.1003.8280.2017.03.014

Chines Journal of Vector Biology and Control ›› 2017, Vol. 28 ›› Issue (3): 251-254.DOI: 10.11853/j.issn.1003.8280.2017.03.014

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Investigation of Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China

JU Wen-dong¹, YANG Fang², JIAO Dan³, WANG Yan-mei¹, CHENG Cheng¹, XU Ning¹, HAN Xi-yu¹, GENG Cong¹, ZHAO De-shun¹, SUN Yi⁴

1 Heilongjiang International Travel Healthcare Center, Harbin 150001, Heilongjiang Province, China;
2 Mudanjiang Entry-Exit Inspection and Quarantine Bureau;
3 Heihe Entry-Exit Inspection and Quarantine Bureau;
4 Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences

Received:2016-12-29 Online:2017-06-20 Published:2017-06-20
Supported by:
Supported by the Research Fund of Heilongjiang Entry-Exit Inspection and Quarantine Bureau(No. 2016HK001, 2015HK002)

黑龙江省牡丹江口岸地区鼠类感染巴尔通体基因多态性调查

鞠文东¹, 杨芳², 焦丹³, 王艳梅¹, 程成¹, 徐宁¹, 韩茜宇¹, 耿聪¹, 赵德顺¹, 孙毅⁴

1 黑龙江国际旅行卫生保健中心, 哈尔滨 150001;
2 牡丹江出入境检验检疫局, 黑龙江牡丹江 157000;
3 黑河出入境检验检疫局, 黑龙江黑河 164300;
4 军事医学科学院微生物流行病研究所, 北京 100071

作者简介:鞠文东,男,主任医师,主要从事卫生检疫方面的研究,Email:juwend@163.com
基金资助:
黑龙江出入境检验检疫局科研基金（2016HK001，2015HK002）

Abstract

Abstract: Objective To investigate Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China. Methods A total of 33 rodents were captured by night trapping and cage method, and their spleen were aseptically collected to detect Bartonella by PCR. The positive samples obtained were further identified through gene sequencing. Results The results showed that 6 positive samples of Bartonella in all rodents,infection prevalence was 18.18%, 5 of which were isolated from Apodemus agrarius, one from Rattus norvegicus. Results showed that at least 4 subtypes of Bartonella were found at Mudanjiang airport, including B. taylorii, B. phoceensis, B. grahamii that are pathogenic to humans, as well as an undetermined species. The results suggested the genotype diversity of Bartonella at Mudanjiang airport. Conclusion The prevalence of Bartonella infection in rodents at Mudanjiang airport poses a risk of transmission to human, and appropriate control measures should be taken into consideration.

Key words: Mudanjiang airport, Rodents, Bartonella, Diversity

摘要： 目的调查黑龙江省牡丹江口岸地区鼠类巴尔通体感染情况。方法 2014年6月，在牡丹江口岸草地及绿化带利用夹夜法和鼠笼法共捕获鼠类33只，采集其脾样本；采用PCR方法检测鼠类巴尔通体感染情况，将阳性样本进行基因测序鉴定。结果共检出巴尔通体核酸阳性样本6份，感染率为18.18%，其中5份分离自黑线姬鼠，1份分离自褐家鼠。牡丹江口岸至少存在4种基因型巴尔通体，包括Bartonella taylorii、B. phoceensis、对人类有致病性的B. grahamii及未定种巴尔通体，提示牡丹江口岸存在巴尔通体基因型多样性的特征。结论黑龙江省牡丹江口岸鼠类存在巴尔通体感染，且携带人类致病性的巴尔通体，对人群有致病风险，应加强口岸地区的鼠类防控能力。

关键词: 牡丹江口岸, 鼠类, 巴尔通体, 多样性

CLC Number:

S443
Q939.93

JU Wen-dong, YANG Fang, JIAO Dan, WANG Yan-mei, CHENG Cheng, XU Ning, HAN Xi-yu, GENG Cong, ZHAO De-shun, SUN Yi. Investigation of Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China[J]. Chines Journal of Vector Biology and Control, 2017, 28(3): 251-254.

鞠文东, 杨芳, 焦丹, 王艳梅, 程成, 徐宁, 韩茜宇, 耿聪, 赵德顺, 孙毅. 黑龙江省牡丹江口岸地区鼠类感染巴尔通体基因多态性调查[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 251-254.

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Investigation of Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China

黑龙江省牡丹江口岸地区鼠类感染巴尔通体基因多态性调查

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