Investigation of mosquito population density and insecticide resistance of Culex pipiens pallens for International Horticultural Exposition park and surrounding area in Tangshan city

doi:10.11853/j.issn.1003.8280.2018.04.007

Abstract

Abstract:

Objective To investigate the change in population density of mosquitoes and the resistance to commonly used insecticides against larvae and adults of Culex pipiens pallens in Tangshan city, and to provide a scientific basis for mosquito control for 2016 International Horticultural Exposition. Methods The mosquito lamp method was used to determine the population density of mosquito adults for International Horticultural Exposition park and surrounding area during 2013 to 2016. The larval immersion method and the adult exposure tube method were used to determine the resistance of Cx. pipiens pallens to commonly used insecticides. The DPS software was used to calculate the median lethal concentration and its 95%CI, virulence regression equation and Chi-square values. The resistance level of different insecticides was compared with Wilcoxon H test by SPSS 17.0 software. Results The average density of mosquitoes was 4.641 per lamp hour in Tangshan city from 2013 to 2016, with the predominant species Cx. pipiens pallens. The highest average density was found in the barn among all habitats (10.556 per lamp hour). Culex pipiens pallens were the predominant species in all surveillance areas. The population density of mosquitoes showed a single-peak throughout the year, peaking in July, being 10.854 per lamp hour. Larval Cx. pipiens pallens were susceptible to DDT, cypermethrin, temephos, chlorpyrifos, tetramethrin, bendiocarb and pyriproxyfen, with the resistance ratio of 0.88, 1.94, 0.82, 0.50, 2.18, 0.53, and 0.02, respectively. All of tested larval mosquitoes were intermediately resistant to bifenthrin with the resistance ratio of 14.00 and highly resistant to ethofenprox with the resistance ratio of 57.00. Culex pipiens pallens adults showed a mortality of 25.00% when contacted deltamethrin for 1 h and mortality of 50.00% when contacted permethrin for 3 h, all tested adults were resistant to deltamethrin and permethrin. Culex pipiens pallens adults experienced 100% mortality when contacted propoxur for 2 h, which was considered susceptible. Conclusion The predominent species were Cx. pipiens pallens, density peaked in July, when comprehensive control measures should be taken. More considerations are warranted about how to choose carbamate, organophosphorus and organochlorine insecticides, prevent or slow down the occurrence of the resistance.

Key words: Mosquito, Population density, Culex pipiens pallens, Insecticide resistance

摘要：

目的掌握河北省唐山市蚊类种群密度和淡色库蚊对常用杀虫剂的抗药性水平，为2016年世界园艺博览会（世园会）的蚊虫防制提供依据。方法 2013-2016年在世园会园区及周边区域采用诱蚊灯法监测成蚊密度；分别采用浸渍法和接触筒法测定淡色库蚊幼虫和成虫对常用杀虫剂的抗药性；采用DPS软件计算半数致死浓度及其95%CI、毒力回归方程和χ²值，不同杀虫剂抗性水平采用SPSS 17.0软件的Wilcoxon H检验进行比较。结果唐山市2013-2016年蚊虫平均密度为4.641只（灯/·h），淡色库蚊为优势种；以牲畜棚的平均蚊密度最高，为10.556只（灯/·h），各监测生境均以淡色库蚊为优势种；蚊密度分布呈单峰曲线，7月达全年最高峰，蚊密度为10.854只（灯/·h）。淡色库蚊幼虫对DDT、氯氰菊酯、双硫磷、毒死蜱、胺菊酯、恶虫威和吡丙醚敏感，抗性倍数分别为0.88、1.94、0.82、0.50、2.18、0.53和0.02倍；对联苯菊酯和醚菊酯分别产生中度和高度抗性，抗性倍数分别为14.00和57.00倍。淡色库蚊成虫接触溴氰菊酯1 h、氯菊酯3 h和残杀威2 h的死亡率分别为25.00%、50.00%和100%。结论淡色库蚊为优势蚊种，各生境蚊密度均在7月达到最高峰；在高峰期采取综合防制措施，应选用氨基甲酸酯类、有机磷类和有机氯类杀虫剂，避免或减缓抗药性的产生。

关键词: 蚊虫, 种群密度, 淡色库蚊, 抗药性

CLC Number:

YUN Ling, WANG Fu-cai, ZHANG Qiu-fen, GAO Qing-hua, WANG Xiu-ying, LI Shu-shuang, SHANG Xiu-li, CHAO Yi-shu, LIU Yuan-yuan, DONG Rong-xuan. Investigation of mosquito population density and insecticide resistance of Culex pipiens pallens for International Horticultural Exposition park and surrounding area in Tangshan city[J]. Chines Journal of Vector Biology and Control, 2018, 29(4): 351-354.

运玲, 王福才, 张秋芬, 高庆华, 王秀英, 李树双, 商秀丽, 晁亦舒, 刘媛媛, 董荣轩. 唐山市世界园艺博览会园区和周边区域蚊类密度及淡色库蚊的抗药性研究[J]. 中国媒介生物学及控制杂志, 2018, 29(4): 351-354.

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