Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (1): 17-20.DOI: 10.11853/j.issn.1003.4692.2016.01.005

• Original reports • Previous Articles     Next Articles

DNA barcoding identification of Mus cervicolor, a non recorded mouse species in China

SHAN Zhen-ju1, LIU De-xing1, YUE Qiao-yun1,2, WEI Xiao-ya1   

  1. 1. Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, Guangdong Province, China;
    2. Technical Center of Guangdong Entry-Exit Inspection and Quarantine Bureau
  • Received:2015-08-19 Online:2016-02-20 Published:2016-02-20
  • Supported by:

    Supported by the National Science and Technology Support Program (No. 2012BAK11B05), Administration of Quality Supervision, Inspection and Quarantine Support Program (No. 2015IK067, 2015IK069) and Guangdong Province Support Program (No. 2015A050502009)

口岸截获国内未见分布种褐小家鼠的DNA条形码鉴定

单振菊1, 刘德星1, 岳巧云1,2, 魏晓雅1   

  1. 1. 中山出入境检验检疫局, 广东 中山 528403;
    2. 广东出入境检验检疫局检验检疫技术中心, 广州 510623
  • 通讯作者: 岳巧云,Email:yueqy@zs.gdciq.ov.cn
  • 作者简介:单振菊,女,硕士,农艺师,从事媒介生物鉴定工作,Email:173465531@qq.com;刘德星,男,从事媒介生物的DNA条形码鉴定工作,Email:596217742@qq.com
  • 基金资助:

    国家科技支撑计划课题(2012BAK11B05); 国家质检总局项目(2015IK067,2015IK069); 广东省科技项目(2015A050502009)

Abstract:

Objective DNA barcoding techniques were employed to identify the unknown mouse species intercepted from the dry cassava imported from Cambodia at Zhongshan port, whose morphology was damaged too badly to be identified. Methods Genomic DNA was extracted from the intercepted mouse. Target fragments were amplified with the universal primers LCO1490 and HCO2198 for the metazoan mitochondrial cytochrome oxidaseⅠ (COⅠ), PCR products were sequenced. The sequences were blasted in the NCBI. Results The sequence homology of the DNA barcode fragment between these two samples were 99.8%, and was more than 98.5% identical to the sequence of Mus cervicolor published in NCBI. Mus cervicolor is never recorded in China before, and it was the first time to intercept this species at the Chinese port according to the references. Conclusion The intercepted mouse was M. cervicolor identified with DNA barcodes, a non-recorded species in China. DNA barcoding techniques could break the restrictions of traditional species identification depending on undamaged morphological characteristics, can improve the ability to identify the exotic species, overcome the difficult in lack of identification references and experts.

Key words: Mus cervicolor, Non-distributed species in China, DNA barcoding

摘要:

目的 利用DNA条形码技术对中山口岸从柬埔寨进口木薯干中截获的外部形态特征破坏严重的2只未知鼠样本进行种类鉴定。方法 提取截获鼠样本的基因组DNA,采用动物线粒体细胞色素氧化酶Ⅰ(COⅠ)的通用引物LCO1490和HCO2198扩增目的片段。然后测序、序列分析,与NCBI中的序列进行比对,并选取NCBI中序列相似度高的部分序列构建NJ树。结果 2只鼠的DNA条形码序列相似度高达99.8%,与NCBI中褐小家鼠(Mus cervicolor)的序列相似性最高为98.5%;大于DNA条形码判断物种的相似度98%的阈值。经科技查新,确认为全国口岸首次截获的国内未见分布鼠种。结论 利用DNA条形码技术对截获鼠类进行鉴定,鉴定结果为褐小家鼠,为国内未见分布种。DNA条形码能克服传统物种鉴定依靠完整的外部形态的局限,并能克服鉴定资料、专业人员缺乏等因素带来的困难,有效提高外来物种的种类鉴别力和准确度。

关键词: 褐小家鼠, 国内未见分布种, DNA条形码

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