Abstract:

Objective Through investigation on plague F1 antibody of domestic dogs, determining whether there is the clue of plague epidemic, and providing guidance of plague prevention and control. Methods Three methods were applied for detection of plague F1 antibody on the acquisition of canine sera, they are indirect hemagglutination test (IHA), enzyme linked immunosorbent assay (ELISA), and colloidal gold immunochromatographic test (GICA). Results In Deqin county, 1051 dogs sera were tested. 3 serum samples were IHA positive, 32 were ELISA positive, 14 were GICA positive, the positive rate were 0.29%, 3.04%, 1.33%. None of 73 was positive in Shangri-La county. Conclusion According the clue obtained, it is necessary to conduct further investigation to confirm the existence of plague natural foci, then formulate monitoring scheme.

Key words: Plague, F1 antibody, Dogs

摘要：

目的 对当地家犬进行鼠疫F1抗体调查,了解是否存在动物鼠疫流行线索,为鼠疫防控提供指导性依据。方法 应用间接血凝试验(IHA)、酶联免疫吸附试验(ELISA)、胶体金免疫层析试验(GICA)3种方法对采集的犬血清进行鼠疫F1抗体检测。结果 德钦县1051份犬血清中IHA阳性血清3份,ELISA阳性32份,GICA阳性14份,阳性率分别为0.29%、3.04%和1.33%。香格里拉县73 份血清均为阴性。结论 应依据所获得的线索,继续进行动物鼠疫调查,制定相应的监测方案。

关键词: 鼠疫, F1抗体, 家犬