目的 对2021年北京市顺义区采集的白纹伊蚊标本进行病毒分离和鉴定。方法 将蚊虫研磨液平行接种叙利亚仓鼠肾细胞（BHK-21细胞）和白纹伊蚊卵细胞（C6/36细胞）进行病毒分离,对病毒分离物开展分子生物学鉴定。结果 2021年在北京市顺义区野外采集的白纹伊蚊标本中分离到1株可以引起C6/36细胞病变的病毒分离物（AalDV-8）。病毒基因组编码区核苷酸序列的分析结果显示,AalDV-8病毒为单链DNA病毒,该病毒基因组编码区全长为3 335 nt,共编码2个非结构蛋白（NS1、NS2）和1个衣壳蛋白（VP）,3种蛋白的核苷酸（氨基酸）序列长度分别为2 376 nt（791 aa）、1 092 nt（363 aa）、1 071 nt（356 aa）。经病毒系统进化分析发现,AalDV-8病毒位于Brevihamaparvovirus属病毒进化组,并与中国广州市白纹伊蚊中分离的AalDV-7病毒亲缘关系最近。结论 首次从北京市白纹伊蚊野外种群中分离出Brevihamaparvovirus属病毒。
Objective To isolate and identify viruses in Aedes albopictus specimens from Shunyi District, Beijing, China in 2021. Methods The virus was isolated by parallel inoculation of Syrian hamster kidney cell line BHK-21 and Ae. albopictus C6/36 cells with mosquito grinding liquid, and molecular biological identification was carried out for the virus isolates. Results A virus isolate (AalDV-8) was isolated from Ae. albopictus specimens collected from the field in Shunyi District, Beijing in 2021, which could cause C6/36 cell lesion. The nucleotide sequence analysis of the virus genome coding region showed that AalDV-8 virus was a single-stranded DNA virus with a total length of 3 335 nt, encoding 2 non-structural proteins (NS1 and NS2) and 1 capsid protein (VP). The nucleotide (amino acid) sequence lengths of the three proteins were 2 376 nt (791 aa), 1 092 nt (363 aa), and 1 071 nt (356 aa), respectively. The phylogenetic analysis showed that AalDV-8 virus belonged to Brevihamaparvovirus group and was closely related to AalDV-7 virus isolated from Ae. albopictus in Guangzhou, China. Conclusion This is the first time to isolate Brevihamaparvovirus from the field Ae. albopictus collected from in Beijing.
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