目的 调查北京市丰台区家庭饲养的猫、犬伴侣动物携带的立克次体、巴尔通体等主要动物源和虫媒病原体。方法 在北京市丰台区宠物医院采集宠物猫、犬的抗凝全血,并记录相关流行病学信息。提取全血DNA,用巢式PCR或半巢式PCR方法检测其中的立克次体、无形体科细菌、巴尔通体、柯克斯体、巴贝虫和泰勒虫。结果 共收集猫、犬血液样本95份,其中猫33份,犬62份。其中无感染性症状40份,有疑似感染性症状54份,无症状记录1份。检测结果显示以上病原体均为阴性。结论 北京市丰台区此次采集的猫、犬未发现携带立克次体、巴尔通体、柯克斯体、巴贝虫等病原体,或其血液中病原体载量较低而未检出,家庭饲养的猫、犬伴侣动物携带和传播动物源病原体的风险较低。但由于此次采样数量有限、采样位置较为单一,这一结论需进一步研究确认。
Objective To investigate Rickettsia, Bartonella, and other major animal- and insect-borne pathogens carried by companion animals such as cats and dogs kept in households in Fengtai District, Beijing, China. Methods Anticoagulant whole blood from cats and dogs were collected at pet hospitals in Fengtai District, Beijing, and related epidemiological information was recorded. DNA was extracted from whole blood, and Rickettsia, Anaplasmataceae, Bartonella, Coxiella, Babesia, and Theileria were detected using nested PCR or semi-nested PCR. Results A total of 95 blood samples were collected from cats and dogs, including 33 samples from cats and 62 samples from dogs. There were 40 samples from the animals without symptoms of infection, 54 samples from those with suspected symptoms of infection, and 1 sample without records of symptoms. All samples tested negative for the above pathogens. Conclusions In this survey conducted in Fengtai District, Beijing, no Rickettsia, Bartonella, Coxiella, or Babesia pathogens were found in cats and dogs collected, or the pathogen load in the blood was too low to be detected, indicating a low risk of carrying and transmitting animal-borne pathogens among household companion animals such as cats and dogs. However, due to the limited sample size and the singularity of the sampling site, further research is needed to confirm this conclusion.
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