溴氰菊酯降解菌的筛选和鉴定研究

周秋明, 南春燕, 袁浩, 单文琪, 陶峰, 董昊炜, 彭恒, 马雅军

doi:10.11853/j.issn.1003.8280.2024.05.002

中国媒介生物学及控制杂志 >

2024 , Vol. 35 >Issue 5: 517 - 522

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.05.002

实验研究

溴氰菊酯降解菌的筛选和鉴定研究

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1. 海军军医大学海军医学系/基础医学院病原生物学教研室, 上海 200433

周秋明,女,硕士,讲师,主要从事病媒生物防控工作,E-mail:zhouqm0201@163.com

收稿日期: 2023-10-24

网络出版日期: 2024-10-18

基金资助

上海市卫生健康委员会科研项目计划基金(202240144);上海市加强公共卫生体系建设三年行动计划(2023-2025年)重点学科(GWVI-11.1-13)

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Selection and identification of deltamethrin-degrading bacteria

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1. College of Naval Medicine/Department of Medical Microbiology and Parasitology, College of Basic Medical, Naval Medical University, Shanghai 200433, China

Received date: 2023-10-24

Online published: 2024-10-18

Supported by

Shanghai Municipal Health Commission Research Project Fund (No. 202240144)； the Sixth Round of Three-Year Public Health Action Plan of Shanghai (No. GWVI-11.1-13)

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摘要

目的筛选和鉴定稻田中可降解溴氰菊酯的细菌菌群,并测定其降解效能。方法采用富集驯化、分离纯化获取溴氰菊酯降解菌群,基于形态学和分子生物学特征鉴定其种类。对单个菌落进行16S rRNA全长序列测定和分析,同时利用MiSeq高通量测序技术测定菌群的16S rRNA V4区片段,对序列进行注释和分析,统计细菌物种和属水平的组成和丰度。在溴氰菊酯降解菌中培养3种菊酯类杀虫剂降解菌,根据杀虫剂的残留浓度,计算菌群降解溴氰菊酯、联苯菊酯和三氟氯氰菊酯杀虫剂的效率。结果本研究从稻田水中分离筛选了可降解溴氰菊酯的菌群,单菌落分子鉴定为：广温拟无枝酸菌、鲍氏不动杆菌、屈曲杆菌、红球菌、拟无枝酸菌、苍白杆菌和黄色杆菌。MiSeq高通量分子鉴定结果显示,在属水平丰度>4%的菌群分别为假单胞菌属（26.78%）,不动杆菌属（7.00%）,苍白杆菌属（6.82%）,无色杆菌属（5.00%）,克雷伯菌属（4.39%）和微杆菌属（4.12%）。降解菌群对3种菊酯类杀虫剂的降解率从第2天开始持续增加,第8天（最长观察时间）时达到高峰,分别为76.75%（联苯菊酯）、63.65%（溴氰菊酯）和74.20%（三氟氯氰菊酯）,其中三氟氯氰菊酯在第6天达到平台期。结论成功分离并获得了可降解溴氰菊酯的细菌菌群,其中假单胞菌属种类为优势菌群。降解菌群对菊酯类杀虫剂有广泛的降解效能,该研究为其在现场应用以及促进杀虫剂的快速降解方面提供了基础资料。

关键词： 溴氰菊酯; 降解菌; 降解效能

本文引用格式

周秋明, 南春燕, 袁浩, 单文琪, 陶峰, 董昊炜, 彭恒, 马雅军 . 溴氰菊酯降解菌的筛选和鉴定研究[J]. 中国媒介生物学及控制杂志, 2024 , 35(5) : 517 -522 . DOI: 10.11853/j.issn.1003.8280.2024.05.002

Abstract

Objective To select and identify deltamethrin-degrading bacteria in paddy fields, and to determine their degradation efficiency. Methods A bacterial flora capable of degrading deltamethrin was obtained through domestication, enrichment, isolation, and purification. The species was identified based on morphological and molecular characteristics. For single colonies, the full length of the 16S rRNA gene was sequenced and analyzed, and for the flora, the 16S rRNA V4 region was sequenced using the MiSeq high-throughput method. Sequence annotation and analysis were conducted. Bacterial composition and abundance were described at species and genus levels. The flora was cultured with deltamethrin, bifenthrin, and cyhalothrin separately, and the degrading efficiency was calculated based on the residual concentrations of the insecticides. Results A bacterial flora capable of degrading deltamethrin was obtained from the water of paddy fields. The identified single colonies included Amycolatopsis eurytherma, Acinetobacter baumannii, Ancylobacter sp., Rhodococcus spp., Amycolatopsis spp., Ochrobactrum sp., and Xanthobacter sp. The MiSeq high-throughput sequencing results showed that bacteria >4% in abundance at the genus level included Pseudomonas （26.78%）, Acinetobacter （7.00%）, Ochrobactrum （6.82%）, Achromobacter （5.00%）, Klebsiella（4.39%）, Microbacterium（4.12%）. The degradation rates of the three pyrethroid insecticides by the bacterial flora continued to increase since day 2, peaking on day 8 the longest observation period at 76.75% （bifenthrin）, 63.65% （deltamethrin）, and 74.20% （cyhalothrin； reaching the plateau on day 6）. Conclusions A deltamethrin-degrading bacterial flora was successfully obtained, in which Pseudomonas was the dominant population, showing wide degradation efficiency for pyrethroid insecticides. This study provides basic data for the field application of degrading bacteria and the studies about rapid degradation of insecticides.

Key words： Deltamethrin; Degrading bacteria; Degradation efficiency

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