内蒙古长爪沙鼠病原体监测研究

汪子豪, 常楠, 李晋宇, 李世豪, 郝馨凝, 胡伟超, 韦晓慧, 王晓旭, 刘起勇

doi:10.11853/j.issn.1003.8280.2024.04.007

中国媒介生物学及控制杂志 >

2024 , Vol. 35 >Issue 4: 422 - 428

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.04.007

媒介生物监测

内蒙古长爪沙鼠病原体监测研究

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1. 南京医科大学公共卫生学院, 江苏, 南京 211166;
2. 传染病溯源预警与智能决策全国重点实验室, 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
3. 山东大学齐鲁医学院公共卫生学院, 山东, 济南 250012

汪子豪,男,在读硕士,从事媒介生物及其相关传染病研究工作,E-mail:dyw_i525.1999@foxmail.com

收稿日期: 2024-01-18

网络出版日期: 2024-08-27

基金资助

国家自然科学基金重大项目(32090023);媒介生物监测与控制项目(102393220020020000012)

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Pathogen surveillance results of Meriones unguiculatus in Inner Mongolia Autonomous Region, China

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1. School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China;
2. Department of Vector Biology and Control, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
3. School of Public Health, Cheeloo College Medicine, Shandong University, Jinan, Shandong 250012, China

Received date: 2024-01-18

Online published: 2024-08-27

Supported by

Major Program of National Natural Science Foundation of China (No. 32090023); Vector Surveillance and Control Project (No. 102393220020020000012)

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摘要

目的通过对内蒙古自治区（内蒙古）长爪沙鼠的病原检测,为监测鼠传疾病及其媒介生物控制提供科学依据。方法 2021—2023年每年5、7和9月在内蒙古地区进行野外鼠类采集,对鼠体脾脏提取核酸,应用荧光定量PCR（qPCR）/反转录qPCR（RT-qPCR）技术,进行巴尔通体、大别班达病毒（DBV）、致病性钩端螺旋体、土拉弗朗西斯菌、地方性斑疹伤寒立克次体、恙虫病东方体、嗜吞噬细胞无形体、汉坦病毒检测,并对阳性率最高的巴尔通体进行rpoB基因测序。不同组间阳性率比较采用χ²检验。结果共检测320份长爪沙鼠样本,巴尔通体阳性率为40.00%,DBV阳性率为6.88%,其他病原体阳性率均<5.00%。测序比对结果表明,样品携带的巴尔通体为Bartonella pachyuromydis、克氏巴尔通体（B. clarridgeiae）、罗莎利马巴尔通体（B. rochalimae）、道志巴尔通体（B. doshiae）、格拉汉姆巴尔通体（B. grahamii）、克拉斯诺夫巴尔通体（B. krasnovii）。巴尔通体阳性率在秋季最高,DBV阳性率在春季最高,2种病原体阳性率季节间差异均有统计学意义（χ²=15.274,P<0.001；χ²=7.247,P=0.025）。巴尔通体阳性率在呼伦贝尔市新巴尔虎右旗达赉苏木最高（70.59%）。结论内蒙古地区长爪沙鼠携带多种病原体,特别是巴尔通体和DBV感染率较高。应根据季节调整疾病监测强度,并加强对呼伦贝尔市新巴尔虎右旗达赉苏木等高风险区域的监测。该研究可为鼠源疾病的风险评估和预测预警提供基础。

关键词： 长爪沙鼠; 病原检测; 巴尔通体; 大别班达病毒

本文引用格式

汪子豪, 常楠, 李晋宇, 李世豪, 郝馨凝, 胡伟超, 韦晓慧, 王晓旭, 刘起勇 . 内蒙古长爪沙鼠病原体监测研究[J]. 中国媒介生物学及控制杂志, 2024 , 35(4) : 422 -428 . DOI: 10.11853/j.issn.1003.8280.2024.04.007

Abstract

Objective To detect pathogens in Meriones unguiculatus in the Inner Mongolia Autonomous Region (Inner Mongolia), China, so as to provide a scientific basis for monitoring rodent-borne diseases and vector control. Methods Field rodents were collected in Inner Mongolia in May, July, and September from 2021 to 2023. Nucleic acids were extracted from the spleen of the rodents to detect Bartonella, Dabie bandavirus (DBV), Rickettsia typhi, Anaplasma phagocytophilum, Orientia tsutsugamushi, Francisella tularensis, pathogenic Leptospira, and Orthohantavirus with quantitative PCR (qPCR)/reverse transcription qPCR. The most prevalent pathogen, Bartonella, was further analyzed through rpoB gene sequencing. The positive rates were compared between groups using the Chi-square test. Results A total of 320 samples of M. unguiculatus were tested, of which 40.00% were positive for Bartonella, 6.88% were positive for DBV, and <5.00% were positive for any other pathogen. Sequence alignment detected the presence of various Bartonella species, including B. pachyuromydis, B. clarridgeiae, B. rochalimae, B. doshiae, B. grahamii, and B. krasnovii. The positive rate differed significantly in different seasons for Bartonella (highest in autumn, χ²=15.274, P<0.001) and for DBV (highest in spring, χ²=7.247, P=0.025). The highest positive rate (70.59%) of Bartonella was found in Dalai Sumu, New Barag Right Banner, Hulun Buir. Conclusions M. unguiculatus in Inner Mongolia carry various pathogens, with high infection rates of Bartonella and DBV. It is necessary to adjust disease surveillance intensity according to seasons, and strengthen surveillance in high-risk areas such as Dalai Sumu, Hulun Buir. This research provides a reference for risk assessment and early warning of rodent-borne diseases.

Key words： Meriones unguiculatus; Pathogen Detection; Bartonella; Dabie bandavirus

参考文献

[1] Li JM, Li LM, Shi JF,et al. Prevalence of Leptospira in murine in China:A systematic review and meta-analysis[J]. Front Vet Sci,2022,9:944282. DOI:10.3389/fvets.2022.944282.
[2] Liu QY. Reported cases of vector-borne diseases in China,2005-2020:Epidemic trend,challenges in prevention and control,and related coping strategies[J]. Chin J Vector Biol Control,2022,33(1):1-7. DOI:10.11853/j.issn.1003.8280.2022.01.001.(in Chinese)刘起勇. 2005—2020年我国媒介生物传染病报告病例:流行趋势、防控挑战及应对策略[J]. 中国媒介生物学及控制杂志,2022,33(1):1-7. DOI:10.11853/j.issn.1003.8280.2022.01.001.
[3] Gu XL, Su WQ, Zhou CM,et al. SFTSV infection in rodents and their ectoparasitic chiggers[J]. PLoS Negl Trop Dis,2022,16(8):e0010698. DOI:10.1371/journal.pntd.0010698.
[4] Wang W, Wang MR, Lin XD,et al. Ongoing spillover of Hantaan and Gou hantaviruses from rodents is associated with hemorrhagic fever with renal syndrome (HFRS) in China[J]. PLoS Negl Trop Dis,2013,7(10):e2484. DOI:10.1371/journal.pntd.0002484.
[5] Liu QY. Epidemic profile of vector-borne diseases and vector control strategies in the new era[J]. Chin J Vector Biol Control,2019,30(1):1-6,11. DOI:10.11853/j.issn. 1003. 8280. 2019. 01.001.(in Chinese)刘起勇. 新时代媒介生物传染病形势及防控对策[J]. 中国媒介生物学及控制杂志,2019,30(1):1-6,11. DOI:10.11853/j.issn.1003.8280.2019.01.001.
[6] Rupasinghe R, Chomel BB, Martínez-López B. Climate change and zoonoses:A review of the current status,knowledge gaps,and future trends[J]. Acta Trop,2022,226:106225. DOI:10.1016/j.actatropica.2021.106225.
[7] Beermann S, Dobler G, Faber M,et al. Impact of climate change on vector- and rodent-borne infectious diseases[J]. J Health Monit,2023,8Suppl 3:S33-61. DOI:10.25646/11401.
[8] Si XY, Bai GH, Song LT,et al. An analysis of rodent surveillance data in human settlements of Inner Mongolia,China,in 2017-2019[J]. Chin J Vector Biol Control,2021,32(5):586-589. DOI:10.11853/j.issn.1003.8280.2021.05.015.(in Chinese)司晓艳,白国辉,宋利桃,等. 内蒙古自治区人居环境2017—2019年鼠类监测数据分析[J]. 中国媒介生物学及控制杂志,2021,32(5):586-589. DOI:10.11853/j.issn. 1003. 8280. 2021. 05.015.
[9] Hao JY, Zhu MD, Yu ZH. Research on landscape features of plague natural foci at Inner Mongolia border area[J]. Port Health Control,2010,15(6):49-50. (in Chinese)郝静云,朱明达,于占海. 内蒙古边境地区鼠疫自然疫源地景观特征的研究[J]. 口岸卫生控制,2010,15(6):49-50.
[10] Li JY, Zhang ZB, Fan MG,et al. Analysis of plague surveillance results in the plague foci of Meriones unguiculatus in Inner Mongolia from 2004 to 2013[J]. Inner Mongolia Med J,2014,46(9):1082-1085. DOI:10.16096/j.cnki.nmgyxzz.2014.09.080.(in Chinese)李建云,张忠兵,范蒙光,等. 内蒙古2004年至2013年长爪沙鼠鼠疫疫源地鼠疫监测结果分析[J]. 内蒙古医学杂志,2014,46(9):1082-1085. DOI:10.16096/j.cnki.nmgyxzz.2014.09.080.
[11] Hu YH, Wang SY, Li JY,et al. Analysis of plague surveillance in Inner Mongolia Autonomous Region,China,2020[J]. Chin J Vector Biol Control,2022,33(3):418-425. DOI:10.11853/j.issn.1003.8280.2022.03.020.(in Chinese)胡艳红,王姝懿,李建云,等. 内蒙古自治区2020年鼠疫监测调查与分析[J]. 中国媒介生物学及控制杂志,2022,33(3):418-425. DOI:10.11853/j.issn.1003.8280.2022.03.020.
[12] Krügel M, Król N, Kempf VAJ,et al. Emerging rodent-associated Bartonella:A threat for human health?[J]. Parasit Vectors,2022,15(1):113. DOI:10.1186/S13071-022-05162-5.
[13] Zhang LN, Meng JY. Overview of wildlife resource in Inner Mongolia[J]. North Econ, 2002(S1):352-352. (in Chinese)张琳娜,孟建宇. 内蒙古野生动物资源概况[J]. 北方经济,2002(S1):352-352.
[14] Jardine C, Waldner C, Wobeser G,et al. Demographic features of Bartonella infections in Richardson's ground squirrels (Spermophilus richardsonii)[J]. J Wildl Dis,2006,42(4):739-749. DOI:10.7589/0090-3558-42.4.739.
[15] Bai Y, Kosoy MY, Ray C,et al. Temporal and spatial patterns of Bartonella infection in black-tailed prairie dogs (Cynomys ludovicianus)[J]. Microb Ecol,2008,56(2):373-382. DOI:10.1007/s00248-007-9355-6.
[16] Paziewska A, Harris PD, Zwolińska L,et al. Differences in the ecology of Bartonella infections of Apodemus flavicollis and Myodes glareolus in a boreal forest[J]. Parasitology,2012,139(7):881-893. DOI:10.1017/S0031182012000170.
[17] Gutiérrez R, Krasnov B, Morick D,et al. Bartonella infection in rodents and their flea ectoparasites:An overview[J]. Vector Borne Zoonotic Dis,2015,15(1):27-39. DOI:10.1089/vbz. 2014.1606.
[18] Jin MZ, Hao GF, Wang J,et al. Investigation on natural infection of rodent-borne pathogens in rodent populations in Urad port area,Inner Mongolia,China in 2015[J]. Chin J Vector Biol Control,2018,29(3):296-297. DOI:10.11853/j.issn. 1003. 8280.2018.03.020.(in Chinese)靳木子,郝广福,王静,等. 内蒙古乌拉特口岸地区2015年鼠类携带病原体情况调查[J]. 中国媒介生物学及控制杂志,2018,29(3):296-297. DOI:10.11853/j.issn. 1003. 8280. 2018. 03.020.
[19] Telfer S, Clough HE, Birtles RJ,et al. Ecological differences and coexistence in a guild of microparasites:Bartonella in wild rodents[J]. Ecology,2007,88(7):1841-1849. DOI:10.1890/06-1004.1.
[20] Čepelka L, Šipoš J, Suchomel J,et al. Can we detect response differences among dominant rodent species to climate and acorn crop in a Central European forest environment?[J]. Eur J Forest Res,2020,139(4):539-548. DOI:10.1007/s10342-020-01267-7.
[21] Sun JM, Lu L, Liu QY, et al. Molecular epidemiological investigation of Bartonella infection in ticks in Zhejiang Province[J]. Chin J Vector Biol Control,2010,21(3):232-234. (in Chinese)孙继民,鲁亮,刘起勇,等. 浙江省蜱中巴尔通体感染的分子流行病学调查[J]. 中国媒介生物学及控制杂志,2010,21(3):232-234.
[22] Chomel BB, Kasten RW. Bartonellosis,an increasingly recognized zoonosis[J]. J Appl Microbiol,2010,109(3):743-750. DOI:10.1111/j.1365-2672.2010.04679.x.
[23] Kosoy M, Hayman DTS, Chan KS. Bartonella bacteria in nature:Where does population variability end and a species start?[J]. Infect Genet Evol,2012,12(5):894-904. DOI:10.1016/j.meegid.2012.03.005.
[24] Liu YY, Li DM, Liu QY,et al. Research progress on Bartonella endocarditis:A review of the literatures[J]. Microbiol China,2015,42(1):192-199. DOI:10.13344/j.microbiol.china.140375.(in Chinese)刘云彦,栗冬梅,刘起勇,等. 巴尔通体感染性心内膜炎的研究进展[J]. 微生物学通报,2015,42(1):192-199. DOI:10.13344/j.microbiol.china.140375.
[25] Li DM, Zhang JZ, Liu QY. Progress of research on Bartonella and related diseases in China[J]. Chin J Zoonoses,2008,24(8):762-765,770. DOI:10.3969/j.issn.1002-2694.2008.08.019.(in Chinese)栗冬梅,张建中,刘起勇. 中国巴尔通体与相关疾病的研究进展[J]. 中国人兽共患病学报,2008,24(8):762-765,770. DOI:10.3969/j.issn.1002-2694.2008.08.019.
[26] Eads DA, Hoogland JL. Factors that affect parasitism of black-tailed prairie dogs by fleas[J]. Ecosphere,2016,7(7):e01372. DOI:10.1002/ecs2.1372.
[27] Li YN, Chen YF, Yun TY,et al. Dominant species of ticks and tick-borne pathogens in the central and western ports areas of the Inner Mongolia Autonomous Region,China,2020[J]. Chin J Vector Biol Control,2022,33(2):216-220. DOI:10.11853/j.issn.1003.8280.2022.02.009.(in Chinese)李伊娜,陈宇飞,云托娅,等. 内蒙古自治区中西部口岸地区2020年蜱类优势种及其病原携带情况的调查研究[J]. 中国媒介生物学及控制杂志,2022,33(2):216-220. DOI:10.11853/j.issn.1003.8280.2022.02.009.
[28] Hu YY, Zhuang L, Liu K,et al. Role of three tick species in the maintenance and transmission of Severe fever with thrombocytopenia syndrome virus[J]. PLoS Negl Trop Dis,2020,14(6):e0008368. DOI:10.1371/journal.pntd.0008368.
[29] Ding F, Zhang WY, Wang LY,et al. Epidemiologic features of severe fever with thrombocytopenia syndrome in China,2011-2012[J]. Clin Infect Dis,2013,56(11):1682-1683. DOI:10. 1093/cid/cit100.
[30] Jin MZ, Yun H, Chen YF,et al. Investigation and analysis of rodent-borne pathogens in Inner Mongolia port areas of China from 2014 to 2015[J]. Chin J Vector Biol Control,2017,28(4):347-349. DOI:10.11853/j.issn.1003.8280.2017.04.010.(in Chinese)靳木子,云华,陈宇飞,等. 2014—2015年内蒙古口岸地区鼠类及其携带病原体调查[J]. 中国媒介生物学及控制杂志,2017,28(4):347-349. DOI:10.11853/j.issn. 1003. 8280. 2017. 04.010.
[31] Chen YF, Jin MZ, Bai X. Investigation on rodents and their pathogens in western port area of Inner Mongolia[J]. Chin J Front Health Quar,2023,46(6):536-538. DOI:10.16408/j. 1004-9770.2023.06.007.(in Chinese)陈宇飞,靳木子,白潇. 内蒙古西部口岸地区鼠类及其携带病原体调查[J]. 中国国境卫生检疫杂志,2023,46(6):536-538. DOI:10.16408/j.1004-9770.2023.06.007.
[32] Zhang F, Wang XH, Yu GW,et al. Detection and genotyping of Francisella tularensis in animal hosts and vectors from six different natural landscape areas,Gansu Province,China[J]. Comput Math Methods Med,2021,2021:6820864. DOI:10. 1155/2021/6820864.
[33] Bounaadja L, Albert D, Chénais B,et al. Real-time PCR for identification of Brucella spp.:A comparative study of IS711,bcsp31 and per target genes[J]. Vet Microbiol,2009,137(1/2):156-164. DOI:10.1016/j.vetmic.2008.12.023.
[34] Sun B, Guo XH, Jiang ZF,et al. Analysis of RNA and gene expression from frozen and paraffin-embedded breast cancer tissues[J]. Chin Clin Oncol,2010,15(7):588-592. DOI:10. 3969/j.issn.1009-0460.2010.07.003.(in Chinese)孙冰,郭晓红,江泽飞,等. 乳腺癌冰冻和石蜡包埋样本的RNA以及基因表达情况的比较研究[J]. 临床肿瘤学杂志,2010,15(7):588-592. DOI:10.3969/j.issn.1009-0460. 2010. 07.003.
[35] Shen ZY, Qu WB, Li ZF,et al. PSC:A system for PCR primer specificity checking and application[J]. Chin J Bioinf,2010,8(2):134-138,141. DOI:10.3969/j.issn.1672-5565.2010.02.009.(in Chinese)申志勇,屈武斌,李稚锋,等. PCR引物特异性核查系统(PSC)的构建与应用[J]. 生物信息学,2010,8(2):134-138,141. DOI:10.3969/j.issn.1672-5565.2010.02.009.
[36] Bai XQ, Yang RR, Zeng YL. Effects of different primers and annealing temperatures on the amplification efficiency of real-time PCR reference gene β- actin in the halophyte Halostachys caspica[J]. Xinjiang Agric Sci,2017,54(2):352-360. DOI:10. 6048/j.issn.1001-4330.2017.02.019.(in Chinese)白雪芹,杨瑞瑞,曾幼玲. 不同引物对和退火温度对盐生植物盐穗木内参基因β- actin扩增效率的影响[J]. 新疆农业科学,2017,54(2):352-360. DOI:10.6048/j.issn.1001-4330. 2017. 02.019.
[37] Huang YH, Hu XX, Xu WZ,et al. The factors affecting the efficiency of mutiplex PCR[J]. Hereditas,2003,25(1):65-68. DOI:10.3321/j.issn:0253-9772.2003.01.015.(in Chinese)黄银花,胡晓湘,徐慰倬,等. 影响多重PCR扩增效果的因素[J]. 遗传,2003,25(1):65-68. DOI:10.3321/j.issn:0253-9772. 2003.01.015.

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