江西省吉安市微小扇头蜱携带病原菌检测及其分型研究

曾予, 刘志云, 段立科, 侯学霞, 张琳, 贺丽娟, 郝琴

doi:10.11853/j.issn.1003.8280.2024.03.022

中国媒介生物学及控制杂志 >

2024 , Vol. 35 >Issue 3: 381 - 388

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.03.022

调查研究

江西省吉安市微小扇头蜱携带病原菌检测及其分型研究

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1. 传染病溯源预警与智能决策全国重点实验室, 中国疾病预防控制中心传染病预防控制所螺旋体病控制室, 北京 102206;
2. 吉安市中心人民医院(上海市东方医院吉安医院)检验科, 江西吉安 343000

曾予,女,在读硕士,主要从事蜱媒病原菌检测工作,E-mail： eurie0602@163.com;刘志云,女,副主任技师,主要从事医学免疫学检验工作,E-mail： liuzhiyun0207@126.com

收稿日期: 2024-01-08

网络出版日期: 2024-06-29

基金资助

中国疾病预防控制中心传染病预防控制所自主课题（32055）；国家“十三五”科技重大专项（2017ZX10303404006003）；2023年吉安市科技计划指导项目（20233-043438）

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Detection and genotyping of pathogenic bacteria carried by Rhipicephalus microplus in Ji’an,Jiangxi Province,China

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1. Department of Spirochetosis Control, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Inspection Department of Ji'an Central People's Hospital (Ji'an Hospital of Shanghai East Hospital), Ji'an, Jiangxi 343000, China

Received date: 2024-01-08

Online published: 2024-06-29

Supported by

Independent Research Project of National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (No. 32055); National Science and Technology Major Project (No. 2017ZX10303404006003); 2023 Ji’an Guiding Science and Technology Plan Project (No. 20233-043438)

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摘要

目的了解江西省吉安市寄生蜱种类及其病原菌携带情况。方法 2022年6月-2023年6月在吉安市永新、吉安和永丰县的牛体表采集417只寄生蜱,通过形态学和16S rDNA特异基因对其进行种类鉴定。并采用普通PCR和巢式PCR检测蜱体内6类蜱传病原菌携带状况,包括伯氏疏螺旋体（B.b）、米氏疏螺旋体（B.m）、斑点热群立克次体（SFGR）、查菲埃立克体（EC）、贝纳柯克斯体（C.b）和嗜吞噬细胞无形体（AP）,对阳性产物进行测序分析。结果 417只寄生蜱均为微小扇头蜱,其中有58只蜱携带蜱传病原菌,总阳性率为13.91%。3县中,永新县检出B.b、SFGR和C.b,其中B.b的阳性率最高,为8.99%；吉安县检出B.b、EC和C.b,其中C.b的阳性率最高,为9.21%；永丰县检出B.b、SFGR、C.b和AP,其中SFGR的阳性率最高,为10.32%。此外,3县蜱均存在多种蜱传病原菌的复合感染现象,均表现为B.b与其他蜱传病原菌的复合感染。系统进化分析表明,吉安市寄生蜱携带Borrelia yangzensis、B. garinii和B. barvariensis 3种基因型的B.b以及Rickettsia slovaca和Candidatus Rickettsia jingxinensis 2种SFGR。结论吉安市不同县寄生蜱携带的病原菌种类和优势基因型各不相同,且存在多种病原菌复合感染现象,提示当地蜱携带病原菌的情况复杂。应进一步开展蜱传疾病的调查和监测工作,为有效防控蜱传疾病提供科学依据。

关键词： 蜱; 蜱传病原菌; 核酸检测; 序列分析

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曾予, 刘志云, 段立科, 侯学霞, 张琳, 贺丽娟, 郝琴 . 江西省吉安市微小扇头蜱携带病原菌检测及其分型研究[J]. 中国媒介生物学及控制杂志, 2024 , 35(3) : 381 -388 . DOI: 10.11853/j.issn.1003.8280.2024.03.022

Abstract

Objective To investigate parasitic ticks and their pathogenic bacteria in Ji’an City, Jiangxi Province,China.Methods A total of 417 parasitic ticks were collected from the body surface of cattle in Yongxin County, Ji’an County, and Yongfeng County of Ji’an City from June 2022 to June 2023. Tick species was identified by morphological examination and 16S rDNA gene analysis. The collected ticks were examined with traditional PCR and nested PCR for six species of tick-borne pathogens,including Borrelia burgdorferi (B.b), B. miyamotoi (B.m), spotted fever group Rickettsia (SFGR), Ehrlichia chaffeensis (EC), Coxiella burnetii (C.b), and Anaplasma phagocytophilum (AP). Positive samples were sequenced.Results All the 417 ticks were identified as Rhipicephalus microplus, and 58 of them tested positive for the pathogens described, with a positive rate of 13.91%. In Yongxin County, B.b, SFGR, and C.b were detected, and the positive rate of B.b was highest (8.99%). In Ji’an County, B.b, EC, and C.b were detected, and the positive rate of C.b was highest (9.21%). In Yongfeng County, B.b, SFGR, C.b, and AP were detected, and the positive rate of SFGR was highest (10.32%). Additionally, co-infections with B. b and other tick-borne pathogens were present in all the three counties. The phylogenetic analysis showed that the parasitic ticks of Ji’an carried three genotypes of B.b (B. yangzensis, B. garinii, and B. barvariensis) and two types of SFGR (R. slovaca and Candidatus R. jingxinensis).Conclusions The species and predominant genotypes of pathogenic bacteria carried by parasitic ticks vary in different counties of Ji’an, and there is a phenomenon of co-infections with multiple bacteria, indicating that pathogenic bacteria carriage in local ticks is complex. Further study and surveillance should be conducted to prevent and control tick-borne diseases in Jiangxi Province.

Key words： Tick; Tick-borne pathogens; Nucleic acid testing; Sequence analysis

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