东北部分地区蜱携带病原体的调查研究

黄冠鹏, 吴琼, 马悠馨, 毛恩荣, 黄潇杨, 王卓, 赵洪明, 冷国双, 李兵, 王猷金, 吴益民

doi:10.11853/j.issn.1003.8280.2024.03.020

中国媒介生物学及控制杂志 >

2024 , Vol. 35 >Issue 3: 369 - 373

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.03.020

调查研究

东北部分地区蜱携带病原体的调查研究

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1. 北部战区疾病预防控制中心生物安全科, 辽宁沈阳 110034;
2. 长春城投生态环境科技有限公司环境检测研究中心, 吉林长春 130033

黄冠鹏,男,硕士,主治医师,主要从事蜱媒病的检验与监测,E-mail：278887238@qq.com;吴琼,女,博士,高级工程师,主要从事微生物学研究工作,E-mail：wuqiong698@126.com

收稿日期: 2023-12-06

网络出版日期: 2024-06-29

基金资助

国家科技重大专项（2016ZX10004203-001-009）

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An investigation of tick-borne pathogens in parts of northeast China

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1. Department of Biological Safety, Center for Disease Control and Prevention in Northern Theater Command, Shenyang, Liaoning 110034, China;
2. Changchun Chengtou Ecological Environment Technology Co. Ltd, Changchun, Jilin 130033, China

Received date: 2023-12-06

Online published: 2024-06-29

Supported by

National Science and Technology Major Project of China (No. 2016ZX10004203-001-009)

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摘要

目的了解东北3省蜱类携带病原体情况,为蜱传疾病的防控提供参考依据。方法 2012-2013年、2016年在东北11个市（县）采集蜱标本,采用聚合酶链式反应（PCR）法检测斑点热群立克次体（SFGR）和巴尔通体,采用巢式PCR法检测新埃立克体、查菲埃立克体和土拉弗朗西斯菌（土拉菌）,采用实时荧光PCR法检测伯氏疏螺旋体和微小巴贝虫。结果 SFGR、查菲埃立克体、土拉菌、伯氏疏螺旋体和微小巴贝虫检出率分别为9.10%、4.10%、0.70%、14.95%和1.87%,差异有统计学意义（χ²=42.808,P<0.001）,伯氏疏螺旋体和SFGR检出率较高,未检出新埃立克体和巴尔通体。不同蜱种中SFGR、伯氏疏螺旋体及微小巴贝虫的阳性率差异有统计学意义（χ²=12.291,P=0.016；χ²=23.409,P<0.001；χ²=11.592,P=0.028）。结论东北地区蜱类携带多种病原体,以伯氏疏螺旋体、SFGR感染较普遍,不同蜱种携带病原体种类有差异,提示该地区应加强蜱媒病原体监测及调查,为蜱媒传染病的预防与控制提供依据。

关键词： 蜱; 蜱媒病原体; 聚合酶链式反应; 东北地区

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黄冠鹏, 吴琼, 马悠馨, 毛恩荣, 黄潇杨, 王卓, 赵洪明, 冷国双, 李兵, 王猷金, 吴益民 . 东北部分地区蜱携带病原体的调查研究[J]. 中国媒介生物学及控制杂志, 2024 , 35(3) : 369 -373 . DOI: 10.11853/j.issn.1003.8280.2024.03.020

Abstract

Objective To investigate the pathogens carried by ticks in three northeastern provinces of China and provide reference basis for the prevention and control of tick borne diseases.Methods From 2012 to 2013 and in 2016, tick specimens were collected from 11 counties and cities of northeastern China. Spotted fever group rickettsiae (SFGR) and Bartonella by PCR were tested, Candidatus Neoehrlichia mikurensis, Ehrlichia chaffeensis, and Francisella tularensis by nested PCR, and Borrelia burgdorferi and Babesia microti by quantitative real-time PCR.Results The detection rate was 9.10% for SFGR, 4.10% for E. chaffeensis, 0.70% for F. tularensis, 14.95% for Bor. burgdorferi, and 1.87% for Bab. microti, with a significant difference (χ²=42.808, P<0.001). Bor. burgdorferi and SFGR were most frequently detected, while Candidatus Neoehrlichia mikurensis and Bartonella were not found. There were significant differences between tick species in the positive rates of SFGR (χ²=12.291, P=0.016), Bor. burgdorferi (χ²=23.409, P<0.001), and Bab. microti (χ²=11.592, P=0.028).Conclusions Ticks carry a variety of pathogens in northeast China, in which Bor. burgdorferi and SFGR are more common, and pathogen species differ between tick species, suggesting that these areas should strengthen tick-borne pathogen surveillance to inform the prevention and control of tick-borne diseases.

Key words： Tick; Tick-borne pathogen; Polymerase chain reaction; Northeast China

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