目的 调查浙江省东阳市农村居民和宿主动物大别班达病毒[DBV,也称发热伴血小板减少综合征病毒（SFTSV）]的感染率,并分析其影响因素,为发热伴血小板减少综合征（SFTS）疫情的控制提供理论依据。方法 选择3个已有SFTS病例报告的镇街,并按照1∶1的配比方法选取3个地貌相似但无病例报告的镇街,每个镇街选取2个村作为研究区域,采集居民和宿主动物血清。人血清样本通过酶联免疫吸附试验进行SFTSV抗体检测,动物血清样本采用双抗原夹心法进行检测。使用SPSS 18.0软件对数据进行统计分析。组间率的比较采用χ2检验,采用logistic回归对研究对象的危险因素进行分析。结果 检测农村居民1 117人,SFTSV抗体阳性22例,阳性率1.97%,不同性别、职业、年龄组间SFTSV抗体阳性率差异无统计学意义（均P>0.05）；logistic回归分析表明居住村既往有SFTS病例报告、近5年有蜱叮咬史与SFTSV抗体阳性存在统计学关联,比值比（OR）分别为2.994[95%置信区间（CI）：1.277～7.021]和3.563（95%CI：1.227～10.342）,均P<0.05。在羊、犬、牛和猪这4种家养动物中,羊和犬的SFTSV抗体阳性率分别为45.83%（11/24）和7.69%（3/39）,调查的7头牛和20头猪均未检测到SFTSV抗体阳性,不同动物之间抗体阳性率差异有统计学意义（χ2=23.559,P<0.001）。调查鼠类183只,检出SFTSV抗体阳性1只。结论 东阳市部分农村地区的居民和动物宿主均检出SFTSV抗体阳性,既往有病例报告地区抗体检出率高于无病例报告地区,提示东阳市SFTSV的流行范围超过预期,今后在SFTS的防控中除了做好灭蜱工作外,还需要加强动物管理、宣传教育和灭鼠等工作。
Objective To investigate the prevalence of infection with Dabie bandavirus, also know as Severe fever with thrombocytopenia syndrome virus (SFTSV), in rural residents and host animals in Dongyang, Zhejiang Province, China, and analyze its influencing factors, so as to provide a theoretical basis for the control of the epidemic of severe fever with thrombocytopenia syndrome (SFTS).Methods Three townships with reported SFTS cases were selected, and another three townships with similar topographic features but without case reports were selected by 1:1 matching. Two villages were selected from each township as the study area to collect serum samples from residents and host animals. Human serum samples were tested for SFTSV antibodies by enzyme-linked immunosorbent assay, while animal serum samples were tested by double antigen sandwich assay. SPSS 18.0 software was used to analyze the data. The Chi-square test was used for rate comparison. A logistic regression analysis was performed to determine risk factors.Results A total of 1 117 rural residents were examined, of whom 22 were positive for SFTSV antibodies, with a positive rate of 1.97%. There were no significant differences in the positive rate of SFTSV antibodies between sexes, ages, and occupations (all P>0.05). According to the logistic regression analysis, the potential risk factors for SFTSV infection included previous reports of SFTS cases in villages of residence (odds ratio [OR]=2.994, 95% confidence interval [CI]: 1.277-7.021, P<0.05) and a history of tick bites in the last five years (OR=3.563, 95%CI: 1.227-10.342, P<0.05). The positive rates of SFTSV antibodies of sheep, dogs, cattle, and pigs were 45.83% (11/24), 7.69% (3/39), 0 (0/7), and 0 (0/20), respectively, showing a significant difference between them (χ2=23.559, P<0.001). Among 183 rodents tested, 1 was positive for SFTSV antibodies.Conclusions In some rural areas of Dongyang, SFTSV antibodies were detected in both residents and animal hosts, and the antibody detection rate was higher in areas with reported cases than in those without case reports, suggesting that the prevalence of SFTSV infection in Dongyang exceeds expectations. In addition to tick control, future SFTS prevention and control efforts should also focus on strengthening animal management, health promotion and education, and rodent control.
[1] Wu H,Zhang YZ. Research progress in Severe fever with thrombocytopenia syndrome bunyavirus[J]. China Trop Med,2017,17（2）：198-202. DOI：10.13604/j.cnki.46-1064/r.2017. 02.24.（in Chinese） 吴慧,张云智. 发热伴血小板减少综合征布尼亚病毒的研究进展[J]. 中国热带医学,2017,17（2）：198-202. DOI：10.13604/j.cnki.46-1064/r.2017.02.24.
[2] Robles NJC,Han HJ,Park SJ,et al. Epidemiology of Severe fever and thrombocytopenia syndrome virus infection and the need for therapeutics for the prevention[J]. Clin Exp Vaccine Res,2018,7（1）：43-50. DOI：10.7774/cevr.2018.7.1.43.
[3] You EQ,Wang L,Zhang L,et al. Epidemiological characteristics of severe fever with thrombocytopenia syndrome in Hefei of Anhui Province：A population-based surveillance study from 2011 to 2018[J]. Eur J Clin Microbiol Infect Dis,2021,40（5）：929-939. DOI：10.1007/s10096-020-04098-x.
[4] Kobayashi Y,Kato H,Yamagishi T,et al. Severe fever with thrombocytopenia syndrome,Japan,2013-2017[J]. Emerg Infect Dis,2020,26（4）：692-699. DOI：10.3201/eid2604.191011.
[5] Ye XL,Dai K,Lu QB,et al. Infection with Severe fever with thrombocytopenia virus in healthy population：A cohort study in a high endemic region,China[J]. Infect Dis Poverty,2021,10（1）：133. DOI：10.1186/s40249-021-00918-0.
[6] Xu PP,Lyu Y,Geng XY,et al. Seroepidemiology of Severe fever with thrombocytopenia syndrome virus,Lu’an[J]. Mod Prev Med,2015,42（11）：1948-1950. （in Chinese） 徐鹏鹏,吕勇,耿兴义,等. 六安市发热伴血小板减少综合征布尼亚病毒血清流行病学调查[J]. 现代预防医学,2015,42（11）：1948-1950.
[7] Zhou SQ,Ning FJ,Meng SX,et al. Serological investigation of the new virus Bunia infection among healthy population in Penglai City[J]. Mod Prev Med,2015,42（3）：476-478. （in Chinese） 周淑琴,宁福江,孟淑欣,等. 蓬莱市健康人群新布尼亚病毒感染的血清学调查[J]. 现代预防医学,2015,42（3）：476-478.
[8] Hu XQ,Zeng LX,Tian H,et al. Investigation on the infection status of New Bunia virus in Baoji[J]. J Xi’an Jiaotong Univ :Med Sci,2015,36（4）：571-573. DOI：10.7652/jdyxb201504030.（in Chinese） 胡晓倩,曾令霞,田辉,等. 宝鸡市新型布尼亚病毒感染状况调查[J]. 西安交通大学学报:医学版,2015,36（4）：571-573. DOI：10.7652/jdyxb201504030.
[9] Xu ZP,Qian YH,Shi C,et al. Analysis on New Bunia virus infections in Wuxi City[J]. Chin J Dis Control Prev,2014,18（3）：239-242. （in Chinese） 许祝平,钱燕华,施超,等. 无锡市新型布尼亚病毒感染情况调查分析[J]. 中华疾病控制杂志,2014,18（3）：239-242.
[10] Chen C,Li P,Li KF,et al. Animals as amplification hosts in the spread of Severe fever with thrombocytopenia syndrome virus：A systematic review and meta-analysis[J]. Int J Infect Dis,2019,79：77-84. DOI：10.1016/j.ijid.2018.11.017.
[11] Hu HY,Lu YH,Xu ZM,et al. Investigation on the antibody level of Severe fever with thrombocytopenia syndrome bunyavirus in common livestock in Tiantai County[J]. Chin J Health Lab Tec,2021,31（22）：2773-2776. （in Chinese） 胡海燕,陆英骅,许照美,等. 天台县常见家畜新布尼亚病毒抗体水平调查[J]. 中国卫生检验杂志,2021,31（22）：2773-2776.
[12] Cheng J,Zhang L,Hu B,et al. Prevalence and molecular phylogenetic analysis of Severe fever with thrombocytopenia syndrome virus in domestic animals and rodents in Hubei Province,China[J]. Virol Sin,2019,34（5）：596-600. DOI：10.1007/s12250-019-00119-y.
[13] Guo B,Wu AL,Chen K,et al. Distribution of ticks and the carrying status of Dabie bandavirus in Dongyang,Zhejiang Province,China[J]. Chin J Vector Biol Control,2023,34（6）：739-743,748. DOI：10.11853/j.issn.1003.8280.2023.06.006.（in Chinese） 郭斌,吴爱兰,陈康,等. 浙江省东阳市蜱类分布及大别班达病毒携带状况调查[J]. 中国媒介生物学及控制杂志,2023,34（6）：739-743,748. DOI：10.11853/j.issn.1003.8280.2023. 06.006.
[14] Lyu YN,Dou XF,Wang XM,et al. Preliminary investigation on the carriage status of the novel bunyavirus among animals and ticks in Beijing area[J]. Int J Virol,2011,18（2）：33-36. DOI：10.3760/cma.j.issn.1673-4092.2011.02.001.（in Chinese） 吕燕宁,窦相峰,王小梅,等. 北京地区动物及蜱中新型布尼亚病毒携带状况的初步调查与分析[J]. 国际病毒学杂志,2011,18（2）：33-36. DOI：10.3760/cma.j.issn.1673-4092.2011. 02.001.
[15] Li Y,Lan YQ,Liu FM,et al. Species and geographical distribution of ecto-parasitic ticks of rodents in China[J]. Chin J Vector Biol Control,2019,30（6）：682-688. DOI：10.11853/j.issn.1003.8280.2019.06.021.（in Chinese） 李晔,蓝玉清,柳付明,等. 中国啮齿目动物体表寄生蜱种类与地理分布回顾[J]. 中国媒介生物学及控制杂志,2019,30（6）：682-688. DOI：10.11853/j.issn.1003.8280.2019.06.021.
[16] Liu J,Dong N,Zhang YZ. Advances in researches on tick-borne viruses causing human diseases[J]. Chin J Public Health,2020,36（4）：646-649. DOI：10.11847/zgggws1124867.（in Chinese） 刘涓,董娜,张云智. 引起人类疾病的蜱传病毒研究进展[J]. 中国公共卫生,2020,36（4）：646-649. DOI：10.11847/zgggws1124867.
