安徽省2022年小兽携带汉坦病毒基因序列分析

陈晴晴, 侯银续, 陆皖行, 吴小珉, 吴磊, 徐庆华

doi:10.11853/j.issn.1003.8280.2024.03.001

中国媒介生物学及控制杂志 >

2024 , Vol. 35 >Issue 3: 261 - 267

DOI: https://doi.org/10.11853/j.issn.1003.8280.2024.03.001

实验研究

安徽省2022年小兽携带汉坦病毒基因序列分析

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安徽省疾病预防控制中心微生物检验室/消毒杀虫科, 安徽合肥 230601

陈晴晴,女,硕士,主管技师,主要从事自然疫源性疾病相关病原检测工作,E-mail：cqq9966@163.com;侯银续,男,硕士,副主任技师,主要从事媒介生物控制学及生物多样性保育研究,E-mail：houyinx-0551@126.com

收稿日期: 2023-12-19

网络出版日期: 2024-06-29

基金资助

2022年度安徽省卫生健康科研项目（AHWJ2022b016,AHWJ2022b030）

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Gene sequence analysis of Hantavirus carried by small mammals in Anhui Province, China, 2022

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Microbiological Laboratory/Department of Disinfection and Vector Control, Anhui Provincial Center for Disease Control and Prevention, Hefei, Anhui 230601, China

Received date: 2023-12-19

Online published: 2024-06-29

Supported by

2022 Health Project of Anhui Province (No. AHWJ2022b016, AHWJ2022b030)

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摘要

目的了解安徽省小兽携带汉坦病毒情况及病毒基因型特征。方法 2021年9月-2022年12月,安徽省按照《全国病媒生物病原学监测方案（试行）》采集小兽样本,运用反转录实时荧光定量PCR方法检测小兽肺标本汉坦病毒核酸,对阳性标本进行S片段全长扩增、测序及基因序列分析。结果全省16个地市共检测4 075份样本,包括8种小兽,其中褐家鼠、小家鼠、黄胸鼠和黑线姬鼠为优势种,分别占32.15%、21.87%、21.72%和20.86%。汉坦病毒核酸阳性率为1.47%（60/4 075）,包含汉滩病毒和首尔病毒。60份阳性标本通过交叉PCR法扩增获得19条S序列,遗传进化分析显示汉滩病毒属于H5亚型,首尔病毒为S3亚型,序列之间核苷酸相似性为72.00%～100%,氨基酸相似性为83.00%～100%。结论安徽省小兽中存在汉滩病毒和首尔病毒感染,应加强对小兽汉坦病毒的监测工作,为肾综合征出血热传染病防控提供科学依据。

关键词： 肾综合征出血热; 汉坦病毒; 基因型; S片段

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陈晴晴, 侯银续, 陆皖行, 吴小珉, 吴磊, 徐庆华 . 安徽省2022年小兽携带汉坦病毒基因序列分析[J]. 中国媒介生物学及控制杂志, 2024 , 35(3) : 261 -267 . DOI: 10.11853/j.issn.1003.8280.2024.03.001

Abstract

Objective To investigate Hantavirus infection and genotype characteristics in small mammals in Anhui Province, China.Methods From September 2021 to December 2022, small mammal specimens were collected in Anhui Province in accordance with the National Biovector Etiology Surveillance Program (Trial). Hantavirus nucleic acid of small mammal’s lung specimens were detected by reverse transcription real-time fluorescence quantitative PCR, and for positive samples, full-length amplification, sequencing, and sequence analysis of the S fragment were conducted.Results A total of 4 075 specimens of 8 species of samll mammals were examined in 16 cities of Anhui Province. The dominant species were Rattus norvegicus (32.15%), Mus musculus (21.87%), R. tanezumi (21.72%), and Apodemus agrarius (20.86%). The positive rate of hantaviral nucleic acids was 1.47% (60/4 075), including Hantaan virus (HNTV) and Seoul virus (SEOV). From the 60 positive samples, 19 S sequences were obtained by cross-species PCR amplification. Genetic evolution analysis showed that HNTV was H5 and SEOV was S3 subtypes, respectively. The similarity of the nucleotide sequences was 72.00% to 100% and that of amino acid sequences was 83.00% to 100%, respectively.Conclusions HNTV and SEOV infections were present in small mammals in Anhui Province. It is necessary to strengthen Hantavirus surveillance in small mammals to provide a scientific basis for the prevention and control of hemorrhagic fever with renal syndrome.

Key words： Hemorrhagic fever with renal syndrome; Hantavirus; Genotype; S fragment

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