目的对2021年浙江省舟山群岛采集的鼠肺样品进行汉坦病毒全基因组序列分析。方法采用巢式PCR法对汉坦病毒核酸阳性的鼠肺样品进行分段扩增,采用DNAStar的子软件SeqMan 7.1.0.44进行序列拼接,采用MEGA 7.0软件进行序列比对和进化分析。结果成功测定3株汉坦病毒全基因组序列,均为首尔型S3亚型,与浙江省瑞安市,辽宁和山东省等地毒株序列同源性较高；与疫苗株Z37核苷酸及氨基酸序列同源性较高,且与疫苗株Z37的2个糖蛋白及4个核蛋白免疫表位均一致。结论首次报道了浙江省舟山群岛地区分离的汉坦病毒全基因组序列,对该地区肾综合征出血热病毒的流行特征和进化规律研究具有一定的参考价值。
Objective To analyze the whole-genome sequences of Hantavirus in rodent lung samples collected from Zhoushan Islands in Zhejiang province, China in 2021.Methods Nested PCR was used to amplify the Hantavirus nucleic acid in Hantavirus positive rodent lung samples. The sequence was assembled by SeqMan 7.1.0.44 in DNAStar, and the sequence alignment and phylogenetic analysis were performed by MEGA 7.0.Results The whole-genome sequences of three Hantavirus strains were successfully determined, all of which were identified as Seoul (SEOV) S3 subtype, with high homology with strains from Rui'an in Zhejiang, Liaoning, and Shandong. They showed high homology in nucleotide and amino acid sequences and consistent immune epitopes of 2 glycoproteins and 4 nucleoproteins with the vaccine strain Z37.Conclusion This study first reported the whole-genome sequences of Hantavirus isolated from Zhoushan Islands in Zhejiang Province, which provides a reference for the study of the epidemic characteristics of hemorrhagic fever with renal syndrome and the virus evolution patterns in this area.
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