贵阳市白纹伊蚊对3种拟除虫菊酯类杀虫剂的抗药性及击倒抗性基因研究

张燕, 王丹, 周敬祝, 师伟芳, 罗小龙, 孔雪雪, 余好, 管毓威, 胡勇, 梁文琴

doi:10.11853/j.issn.1003.8280.2023.05.003

中国媒介生物学及控制杂志 >

2023 , Vol. 34 >Issue 5: 600 - 606

DOI: https://doi.org/10.11853/j.issn.1003.8280.2023.05.003

实验研究

贵阳市白纹伊蚊对3种拟除虫菊酯类杀虫剂的抗药性及击倒抗性基因研究

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1. 贵州医科大学公共卫生与健康学院/环境污染与疾病监控教育部重点实验室, 贵州贵阳 550025;
2. 贵州省疾病预防控制中心实验中心病媒生物监测科, 贵州贵阳 550004

张燕,女,在读硕士,主要从事白纹伊蚊抗药性研究工作,E-mail:813760864@qq.com

收稿日期: 2023-03-23

网络出版日期: 2023-10-27

基金资助

贵州省科技计划项目（黔科合支撑〔2022〕一般178,黔科合支撑〔2020〕4Y169号）；贵州省传染病预防与控制人才基地科研团队（RCJD2107）

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Resistance to three pyrethroid insecticides and knockdown resistance gene mutations in Aedes albopictus in Guiyang,Guizhou Province,China

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1. School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China;
2. Department of Vector Surveillance, Experimental Center, Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China

Received date: 2023-03-23

Online published: 2023-10-27

Supported by

Plan Project of the Science and Technology in Guizhou Province (No. Qian Ke He [2022] General 178, Qian Ke He [2020]4Y169); Scientific Research Team of Guizhou Provincial Infectious Disease Prevention and Control Talent Base (No. RCJD2107)

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摘要

目的了解贵阳市白纹伊蚊对拟除虫菊酯类杀虫剂的抗性程度并检测击倒抗性基因突变,为该地区白纹伊蚊防治提供科学依据。方法2022年7－8月在贵阳市不同方位采集白纹伊蚊幼虫,带回实验室饲养至F1～F2代,采用幼虫浸渍法和成蚊接触筒法测定其对拟除虫菊酯类杀虫剂的抗药性；单只提取白纹伊蚊成蚊基因组DNA,采用普通PCR扩增后直接测序的方法分析击倒抗性基因突变。χ²检验用于分析各组间突变基因频率差异。结果贵阳市白纹伊蚊幼虫对溴氰菊酯、氯菊酯和高效氯氰菊酯的半数致死浓度（LC₅₀）分别为0.559、0.021和0.012 mg/L,抗性倍数分别为433.33、46.67和16.44倍；0.03%溴氰菊酯、0.4%氯菊酯和0.08%高效氯氰菊酯处理后白纹伊蚊成蚊的死亡率均<80.00%；敏感品系白纹伊蚊在1016、1532和1534基因位点均未检测到突变,自然种群白纹伊蚊在3个位点均检测到击倒抗性基因突变。1016位点有2种等位基因,即野生型GTA（V）（76.35%）和突变型GGA（G）（23.65%）,其敏感表型与抗性表型突变基因频率差异无统计学意义（χ²=1.810,P=0.178）；有3种基因型,即野生型纯合子V/V（58.78%）、野生/突变型杂合子V/G（35.14%）和突变型纯合子G/G（6.08%）。1532位点有2种等位基因,即野生型ATC（I）（99.83%）和突变型ACC（T）（0.17%）；有2种基因型,即野生型纯合子I/I（99.66%）和野生/突变型杂合子I/T（0.34%）。1534位点有2种等位基因,即野生型TTC（F）（48.48%）和突变型TCC（S）（51.52%）,其敏感表型与抗性表型突变基因频率差异无统计学意义（χ²=0.603,P=0.437）；有3种基因型,即野生型纯合子F/F（8.11%）、野生/突变型杂合子F/S（80.74%）和突变型纯合子S/S（11.15%）。结论贵阳市白纹伊蚊幼虫和成蚊对3种拟除虫菊酯类杀虫剂均已产生中高抗性,并发生击倒抗性基因突变,但未发现其基因突变与抗性表型有明显关联；可持续监测该地区白纹伊蚊抗药性水平,指导杀虫剂科学合理使用,以有效防治蚊虫和延缓杀虫剂抗性产生和发展。

关键词： 白纹伊蚊; 抗药性; 击倒抗性基因; 抗性表型

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张燕, 王丹, 周敬祝, 师伟芳, 罗小龙, 孔雪雪, 余好, 管毓威, 胡勇, 梁文琴 . 贵阳市白纹伊蚊对3种拟除虫菊酯类杀虫剂的抗药性及击倒抗性基因研究[J]. 中国媒介生物学及控制杂志, 2023 , 34(5) : 600 -606 . DOI: 10.11853/j.issn.1003.8280.2023.05.003

Abstract

Objective To evaluate the level of resistance of Aedes albopictus to pyrethroid insecticides in Guiyang,China,and identify mutations in the knockdown resistance gene, so as to provide a scientific basis for the control of Ae. albopictus in the region.Methods From July to August 2022,the larvae of Ae. albopictus were captured at different locations of Guiyang and then reared to F1-F2 generations in the laboratory. The resistance to pyrethroid insecticides was determined by using the dipping method for larvae and the World Health Organization tube method for adult mosquitoes. The genomic DNA of individual adult Ae. albopictus mosquito was extracted for PCR amplification and sequencing to detect knockdown resistance gene mutations. The Chi-square test was used to analyze the frequency differences of mutant genes between groups.Results The median lethal concentrations of deltamethrin, permethrin, and beta-cypermethrin against Ae. albopictus larvae in Guiyang were 0.559, 0.021, and 0.012 mg/L, respectively;and the resistance ratios were 433.33, 46.67, and 16.44, respectively. The mortality rates of adult Ae. albopictus mosquitoes exposed to 0.03% deltamethrin, 0.4% permethrin, and 0.08% beta-cypermethrin were all <80%. No mutations were detected at the 1016, 1532, and 1534 loci in the sensitive population of Ae. albopictus. Knockdown resistance gene mutations were found at all the three loci in the natural population of Ae. albopictus. There were two alleles at 1016 locus, which were wild-type GTA (V) (76.35%) and mutant GGA (G) (23.65%), with no statistically significant differences in frequency of mutant genes between the sensitive and resistant phenotypes (χ²=1.810, P=0.178); There were three genotypes at 1016 locus: wild-type homozygous V/V (58.78%), wild/mutant heterozygous V/G (35.14%),and mutant homozygous G/G (6.08%). There were two alleles at 1532 locus, which were wild-type ATC (I) (99.83%) and mutant ACC (T) (0.17%),with two genotypes of wild-type homozygous I/I (99.66%) and wild/mutant heterozygous I/T (0.34%). There were two alleles at 1534 locus, namely wild-type TTC (F) (48.48%) and mutant TCC (S) (51.52%),with no statistically significant difference in frequency of mutant genes between the sensitive and resistant phenotypes (χ²=0.603, P=0.437); There were three genotypes at 1534 locus: wild-type homozygous F/F (8.11%), wild/mutant heterozygous F/S (80.74%),and mutant heterozygous S/S (11.15%).Conclusions Larval and adult Ae. albopictus mosquitoes in Guiyang have developed medium-to-high resistance to the three pyrethroid insecticides and mutations in the knockdown resistance gene,but with no significant association between the mutations and the resistant phenotypes. The level of resistance in the region should be continuously monitored to guide the scientific and rational use of insecticides to effectively control mosquitoes and delay the evolution of insecticide resistance.

Key words： Aedes albopictus; Insecticide resistance; Knockdown resistance gene; Resistance phenotype

参考文献

[1] Meng FX, Wang YG, Feng L, et al. Review on dengue prevention and control and integrated mosquito management in China[J]. Chin J Vector Biol Control, 2015, 26（1）: 4-10. DOI:10.11853/j.issn.1003.4692.2015.01.002.（in Chinese）孟凤霞, 王义冠, 冯磊, 等. 我国登革热疫情防控与媒介伊蚊的综合治理[J]. 中国媒介生物学及控制杂志, 2015, 26（1）: 4-10. DOI:10.11853/j.issn.1003.4692.2015.01.002.
[2] Huang XY, Ma HX, Wang HF, et al. Outbreak of dengue fever in central China, 2013[J]. Biomed Environ Sci, 2014, 27（11）: 894-897. DOI:10.3967/bes2014.125.
[3] Lin HX, Wang XT, Li ZG, et al. Epidemiological characteristics of dengue in mainland China from 1990 to 2019: A descriptive analysis[J]. Medicine （Baltimore）, 2020, 99（36）: e21982. DOI:10.1097/MD.0000000000021982.
[4] Djiappi-Tchamen B, Nana-Ndjangwo MS, Mavridis K, et al. Analyses of insecticide resistance genes in Aedes aegypti and Ae. albopictus mosquito populations from Cameroon[J]. Genes （Basel）, 2021, 12（6）: 828. DOI:10.3390/genes12060828.
[5] Silva JJ, Scott JG. Conservation of the voltage-sensitive sodium channel protein within the Insecta[J]. Insect Mol Biol, 2020, 29（1）: 9-18. DOI:10.1111/imb.12605.
[6] Scott JG. Life and death at the voltage-sensitive sodium channel: Evolution in response to insecticide use[J]. Annu Rev Entomol, 2019,64: 243-257. DOI:10.1146/annurev-ento-011118-112420.
[7] Kasai S, Ng LC, Lam-Phua SG, et al. First detection of a putative knockdown resistance gene in major mosquito vector, Aedes albopictus[J]. Jpn J Infect Dis, 2011, 64（3）: 217-221. DOI:10.7883/yoken.64.217.
[8] Auteri M, La Russa F, Blanda V, et al. Insecticide resistance associated with kdr mutations in Aedes albopictus: An update on worldwide evidences[J]. Biomed Res Int, 2018, 2018: 3098575. DOI:10.1155/2018/3098575.
[9] Wang D, Shi P, Zhao WP, et al. Monitoring and analysis of insecticide resistance of Aedes albopictus in Xingyi and Chishui cities of Guizhou province, China[J]. Chin J Vector Biol Control, 2021, 32（3）: 302-306. DOI:10.11853/j.issn.1003. 8280.2021.03.009.（in Chinese）王丹, 史鹏, 赵文平, 等. 贵州省兴义及赤水市白纹伊蚊抗药性监测分析[J]. 中国媒介生物学及控制杂志, 2021, 32（3）: 302-306. DOI:10.11853/j.issn.1003.8280.2021.03.009.
[10] Kasai S, Caputo B, Tsunoda T, et al. First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe （Italy） and Asia （Vietnam）, 2016: A new emerging threat to controlling arboviral diseases[J]. Euro Surveill, 2019, 24（5）: 1700847. DOI:10.2807/1560-7917.ES.2019.24.5.1700847.
[11] Wei Y, Zheng XL, He S, et al. Insecticide susceptibility status and knockdown resistance （kdr） mutation in Aedes albopictus in China[J]. Parasit Vectors, 2021, 14（1）: 609. DOI:10.1186/s13071-021-05095-5.
[12] Liang WQ, Lin Y, Li H, et al. Resistance of Aedes albopictus to commonly used insecticides in Guiyang city of China[J]. Chin J Hyg Insect Equip, 2018, 24（4）: 348-351. DOI:10.19821/j.1671-2781.2018.04.009.（in Chinese）梁文琴, 林懿, 黎红, 等. 贵阳市白纹伊蚊对常用杀虫剂的抗性研究[J]. 中华卫生杀虫药械, 2018, 24（4）: 348-351. DOI:10.19821/j.1671-2781.2018.04.009.
[13] Wu SH, Zhou XX, Ke XM, et al. Study on sensitivity and knockdown resistance genes of Aedes albopictus to pyrethroid insecticides in Xiamen, Fujian province, China, 2020[J]. Chin J Vector Biol Control, 2022, 33（2）: 177-182. DOI: 10.11853/j.issn.1003.8280.2022.02.003.（in Chinese）伍思翰, 周欣欣, 柯雪梅, 等. 福建省厦门市2020年白纹伊蚊对拟除虫菊酯类杀虫剂敏感性及击倒抗性基因研究[J]. 中国媒介生物学及控制杂志, 2022, 33（2）: 177-182. DOI:10.11853/j.issn.1003.8280.2022.02.003.
[14] Balaska S, Fotakis EA, Kioulos I, et al. Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control[J]. Parasit Vectors, 2020, 13（1）: 328. DOI:10.1186/s13071-020-04204-0.
[15] Wu YY, Liu QM, Qi YP, et al. Knockdown resistance （kdr） mutations I1532T and F1534S were identified in Aedes albopictus field populations in Zhejiang province, central China[J]. Front Cell Infect Microbiol, 2021, 11: 702081. DOI:10.3389/fcimb.2021.702081.
[16] Chen HY, Li KL, Wang XH, et al. First identification of kdr allele F1534S in VGSC gene and its association with resistance to pyrethroid insecticides in Aedes albopictus populations from Haikou city, Hainan Island, China[J]. Infect Dis Poverty, 2016, 5: 31. DOI:10.1186/s40249-016-0125-x.
[17] Yang LJ, Liu DX, Chen J, et al. Detection and analysis of the knockdown resistance gene in the field populations of Aedes albopictus in Zhongshan[J]. Chin J Zoonoses, 2021, 37（2）: 171-175. DOI:10.3969/j.issn.1002-2694.2021.00.005.（in Chinese）杨罗菊, 刘德星, 陈健, 等. 中山市白纹伊蚊现场种群击倒抗性基因检测分析[J]. 中国人兽共患病学报, 2021, 37（2）: 171-175. DOI:10.3969/j.issn.1002-2694.2021.00.005.
[18] Wang GX, Li YS, Li YY, et al. Resistance to deltamethrin and knockdown resistance mutation in Aedes albopictus from Jiangsu province[J]. Chin J Parasitol Parasit Dis, 2022, 40（4）: 468-474, 480. DOI:10.12140/j.issn.1000-7423.2022.04.008.（in Chinese）王冠熙, 李雅姝, 李月月, 等. 江苏省白纹伊蚊对溴氰菊酯抗性及击倒抗性突变分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40（4）: 468-474, 480. DOI:10.12140/j.issn.1000-7423.2022.04.008.
[19] Zhu CY, Zhao CC, Lun XC, et al. Distribution of knockdown resistance genotypes in Aedes albopictus in Jinghong, Yunnan province, China, 2018-2019[J]. Chin J Vector Biol Control, 2020, 31（1）: 7-11. DOI:10.11853/j.issn.1003.8280.2020.01. 002.（in Chinese）朱彩英, 赵春春, 伦辛畅, 等. 云南省景洪市2018－2019年白纹伊蚊击倒抗性基因型分布研究[J]. 中国媒介生物学及控制杂志, 2020, 31（1）: 7-11. DOI:10.11853/j.issn.1003.8280. 2020.01.002.
[20] Li YW, Huang JW, Zhang CM, et al. Knockdown resistance gene mutations of Aedes albopictus from Fuzhou and Putian, Fujian, 2020[J]. China Trop Med, 2021, 21（10）: 952-955, 969. DOI:10.13604/j.cnki.46-1064/r.2021.10.08.（in Chinese）李玉伟, 黄婧雯, 章灿明, 等. 福建省福州市和莆田市2020年白纹伊蚊击倒抗性基因突变分析[J]. 中国热带医学, 2021, 21（10）: 952-955, 969. DOI:10.13604/j.cnki.46-1064/r.2021.10.08.
[21] Zhou XJ, Zhao YH, Liu N, et al. Detection and analysis of kdr resistance mutations in two populations of Aedes albopictus in Beijing[J]. Chin J Hyg Insect Equip, 2021, 27（4）: 304-307. DOI:10.19821/j.1671-2781.2021.04.003.（in Chinese）周小洁, 赵宇晗, 刘念, 等. 北京市两个白纹伊蚊种群kdr抗性突变检测分析[J]. 中华卫生杀虫药械, 2021, 27（4）: 304-307. DOI:10.19821/j.1671-2781.2021.04.003.
[22] Liu HM, Liu LH, Cheng P, et al. Bionomics and insecticide resistance of Aedes albopictus in Shandong, a high latitude and high-risk dengue transmission area in China[J]. Parasit Vectors, 2020, 13（1）: 11. DOI:10.1186/s13071-020-3880-2.
[23] Chen HM, Gao JP, Jiang JY, et al. Detection of the I1532 and F1534 kdr mutations and a novel mutant allele I1532T in VGSC gene in the field populations of Aedes albopictus from China[J]. Chin J Vector Biol Control, 2018, 29（2）: 120-125. DOI:10.11853/j.issn.1003.8280.2018.02.002.（in Chinese）陈翰明, 高景鹏, 姜进勇, 等. 我国白纹伊蚊现场群体击倒抗性基因I1532和F1534突变检测及I1532T突变等位基因报告[J]. 中国媒介生物学及控制杂志, 2018, 29（2）: 120-125. DOI:10.11853/j.issn.1003.8280.2018.02.002.
[24] Li YJ, Zhou GF, Zhong DB, et al. Widespread multiple insecticide resistance in the major dengue vector Aedes albopictus in Hainan province, China[J]. Pest Manag Sci, 2021, 77（4）: 1945-1953. DOI:10.1002/ps.6222.
[25] Zhao CC, Zhu CY, Jia QC, et al. Resistance of Aedes albopictus to commonly used insecticides in different areas of China, 2017-2018[J]. Chin J Vector Biol Control, 2020, 31（2）: 126-132. DOI:10.11853/j.issn.1003.8280.2020.02.002.（in Chinese）赵春春, 朱彩英, 贾清臣, 等. 2017－2018年我国不同区域白纹伊蚊对常用杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志, 2020, 31（2）: 126-132. DOI:10.11853/j.issn.1003.8280. 2020.02.002.
[26] Hou J, Liu QM, Wang JN, et al. Insecticide resistance of Aedes albopictus in Zhejiang province, China[J]. Biosci Trends, 2020, 14（4）: 248-254. DOI:10.5582/bst.2020.03194.

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