贵州省施秉县媒介蜱携带双芽巴贝斯虫的调查研究

吴胜春, 孟娇, 黄健胜, 余福勋, 吴家红, 杨光红, 江佳富, 孙毅, 曹务春, 詹琳

doi:10.11853/j.issn.1003.8280.2023.02.019

中国媒介生物学及控制杂志 >

2023 , Vol. 34 >Issue 2: 254 - 261

DOI: https://doi.org/10.11853/j.issn.1003.8280.2023.02.019

调查研究

贵州省施秉县媒介蜱携带双芽巴贝斯虫的调查研究

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1. 贵州医科大学公共卫生与健康学院, 环境污染与疾病监控教育部重点实验室, 贵州贵阳 550025;
2. 贵州大学医学院, 贵州贵阳 550025;
3. 贵州省人民医院中心实验室, 贵州贵阳 550002;
4. 贵州省疾病预防控制中心, 贵州贵阳 550001;
5. 军事科学院军事医学研究院, 北京 100001

吴胜春,女,苗族,在读硕士,主要从事蜱媒病分子流行病学研究,E-mail：2935769738@qq.com

收稿日期: 2022-10-26

网络出版日期: 2023-04-26

基金资助

国家自然科学基金（82160633，81760605）；贵州省高层次创新型人才（黔科合平台人才-GCC〔2022〕033-1）

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Investigation of Babesia bigemina carried by ticks in Shibing county, Guizhou province, China

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1. School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China;
2. Medical School of Guizhou University, Guiyang, Guizhou 550025, China;
3. Central Laboratory, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China;
4. Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550001, China;
5. Academy of Military Medicine, Academy of Military Sciences, Beijing 100001, China

Received date: 2022-10-26

Online published: 2023-04-26

Supported by

National Natural Science Foundation of China (No. 82160633,81760605);High-level and Innovative Talents of Guizhou Province (No. QKH-GCC[2022]033-1)

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摘要

目的了解贵州省施秉县蜱的双芽巴贝斯虫感染情况。方法于2021－2022年在贵州省施秉县采集散养黄牛体表寄生蜱，经形态学分类后辅以16S rDNA及细胞色素C氧化酶亚基Ⅰ（COX1）基因检测鉴定蜱种类；用梨形虫18S rDNA对样本进行初筛，后用双芽巴贝斯虫顶端膜抗原1（AMA-1）基因进行PCR验证及序列测定，所测序列通过美国国立生物技术信息中心（NCBI）的BLAST进行比对，采用邻接法构建进化树，掌握施秉县蜱携带双芽巴贝斯虫的遗传进化特征。结果共采集蜱样本615只，分子生物学鉴定有2属4种，分别为微小扇头蜱、镰形扇头蜱、长角血蜱和北岗血蜱；从微小扇头蜱体内检测到双芽巴贝斯虫核酸片段，结果表明，AMA-1基因序列与从菲律宾的牛血及苏丹的小亚璃眼蜱中检出的双芽巴贝斯虫有较近的亲缘关系，18S rDNA基因序列与印度的双芽巴贝斯虫亲缘关系最近。结论施秉县散养家畜体表寄生蜱体内携带双芽巴贝斯虫的核酸片段，提示施秉县媒介蜱可能存在巴贝斯虫感染。研究结果可为该地区开展蜱传原虫病防治工作提供参考数据。

关键词： 寄生蜱; 梨形虫; 双芽巴贝斯虫; 贵州; 施秉

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吴胜春, 孟娇, 黄健胜, 余福勋, 吴家红, 杨光红, 江佳富, 孙毅, 曹务春, 詹琳 . 贵州省施秉县媒介蜱携带双芽巴贝斯虫的调查研究[J]. 中国媒介生物学及控制杂志, 2023 , 34(2) : 254 -261 . DOI: 10.11853/j.issn.1003.8280.2023.02.019

Abstract

Objective To investigate the infection of Babesia bigemina in ticks at Shibing county in Guizhou province of China. Methods Ticks were collected from the body surface of free-range cattle in Shibing from 2021 to 2022. The 16S rDNA and cytochrome c oxidase subunit Ⅰ(COX1) gene detection techniques were used to identify the tick species after preliminary morphological classification. The samples were initially screened with Piroplasma 18S rDNA gene, followed by PCR verification and sequencing with B. bigemina apical membrane antigen-1 (AMA-1) gene. The resulting sequences were aligned using BLAST at the National Center for Biotechnology Information (NCBI) website. Phylogenetic trees were constructed by using the neighbor-joining method to understand the genetic evolution characteristics of tick-borne B. bigemina in Shibing. Results In this study, 615 ticks were collected and assigned to 2 genera and 4 species, including Rhipicephalus microplus, R. haemaphysaloides, Haemaphysalis longicornis, and H. kitaokai. Nucleic acid fragments of B. bigemina were detected in R. microplus. The sequencing result showed that the AMA-1 gene sequence was closely related to the B. bigemina detected from bovine blood in the Philippines and the tick of Hyalomma anatolicum in Sudan, and the 18S rDNA sequence was most closely related to B. bigemina in India. Conclusions The parasitic ticks on the body surface of free-range cattle in Shibing carry the nucleic acid fragments of B. bigemina, suggesting infection of the vector ticks in Shibing with B. bigemina. Our results provide reference data for the prevention and control of tick-borne protozoonosis in this area.

Key words： Parasitic tick; Piroplasma; Babesia bigemina; Guizhou; Shibing

参考文献

[1] 邓国藩,姜在阶. 中国经济昆虫志. 第三十九册.蜱螨亚纲.硬蜱科[M]. 北京:科学出版社,1991:34-43.Deng GF,Jiang ZJ. Economic insect fauna of China. Fasc. 39(Acari:Ixodidae)[M]. Beijing:Science Press,1991:34-43. (in Chinese)
[2] 刘玮. 中国新发蜱媒病原体概况[J]. 传染病信息,2017,30(1):11-14. DOI:10.3969/j.issn.1007-8134.2017.01.005.Liu W. Emerging tick borne agents in China[J]. Infect Dis Inf,2017,30(1):11-14. DOI:10.3969/j.issn.1007-8134.2017.01. 005.(in Chinese)
[3] 陈泽. 中国蜱类的系统分类及两种硬蜱的生物学特性分析[D]. 石家庄:河北师范大学,2010.Chen Z. Taxonomic and systematic research of Chinese ticks and biological characteristic analysis of two hard tick species[D]. Shijiazhuang:Hebei Normal University,2010. (in Chinese)
[4] Niu QL,Liu ZJ,Yu PF,et al. Genetic characterization and molecular survey of Babesia bovis,B. bigemina and B. ovata in cattle,dairy cattle and yaks in China[J]. Parasit Vectors,2015,8:518. DOI:10.1186/s13071-015-1110-0.
[5] 刘启生. 牛巴贝斯虫病实时荧光PCR诊断方法的建立及其初步应用研究[D]. 乌鲁木齐:新疆农业大学,2013.Liu QS. Establishment of real-time fluorescence PCR methods for diagnosing bovine Babesiosis and its applications[D]. Urumqi:Xinjiang Agricultural University,2013. (in Chinese)
[6] He L,Bastos RG,Sun YL,et al. Babesiosis as a potential threat for bovine production in China[J]. Parasit Vectors,2021,14(1):460. DOI:10.1186/s13071-021-04948-3.
[7] 周贵,田琴,梅世慧. 一例牛双芽巴贝斯虫、大肠杆菌混合感染的实验室诊断[J]. 黑龙江畜牧兽医,2022(20):100-103,145. DOI:10.13881/j.cnki.hljxmsy.2021.10.0255.Zhou G,Tian Q,Mei SH. Laboratory diagnosis of a case of co-infection of bovine Babesia bigemina and Escherichia coli[J]. Heilongjiang Anim Sci Vet Med,2022(20):100-103,145. DOI:10.13881/j.cnki.hljxmsy.2021.10.0255.(in Chinese)
[8] 汤晓辛,王英,杨庆伟,等. 施秉云台山白云岩喀斯特地区豆科植物资源调查[J]. 湖北农业科学,2016,55(5):1183-1185. DOI:10.14088/j.cnki.issn0439-8114.2016.05.024.Tang XX,Wang Y,Yang QW,et al. The resources survey of leguminosae plant in dolomite Karst area,Yuntai Mountain in Shibing[J]. Hubei Agric Sci,2016,55(5):1183-1185. DOI:10.14088/j.cnki.issn0439-8114.2016.05.024.(in Chinese)
[9] 周玮. 贵州省施秉县旅游资源分析与发展策略[J]. 旅游纵览,2015(16):119-121.Zhou W. Analysis and development strategy of tourism resources in Shibing county of Guizhou province[J]. Tourism Surv,2015(16):119-121. (in Chinese)
[10] Chen Z,Li YQ,Ren QY,et al. Dermacentor everestianus Hirst,1926(Acari:Ixodidae):Phylogenetic status inferred from molecular characteristics[J]. Parasitol Res,2014,113(10):3773-3779. DOI:10.1007/s00436-014-4043-1.
[11] Chitimia L,Lin RQ,Cosoroaba I,et al. Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes[J]. Exp Appl Acarol,2010,52(3):305-311. DOI:10.1007/s10493-010-9365-9.
[12] 中华人民共和国国家卫生和计划生育委员会.WS/T 564-2017巴贝虫病诊断[S/OL]. (2017-08-01)[2022-09-16]. http://www.nhc.gov.cn/ewebeditor/uploadfile/2017/08/20170811160825135.pdf. National Health and Family Planning Commission of the People's Republic of China. WS/T 564-2017 Diagnosis of babesiosis[S/OL]. (2017-08-01)[2022-09-16]. http://www.nhc.gov.cn/ewebeditor/uploadfile/2017/08/20170811160825135.pdf. (in Chinese)
[13] Sivakumar T,Altangerel K,Battsetseg B,et al. Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay[J]. Vet Parasitol,2012,187(1/2):17-22. DOI:10.1016/j.vetpar.2012.01.008.
[14] Lu M,Meng C,Zhang B,et al. Prevalence of spotted fever group Rickettsia and Candidatus Lariskella in multiple tick species from Guizhou province,China[J]. Biomolecules,2022,12(11):1701. DOI:10.3390/biom12111701.
[15] 刘联坤,郭建军. 贵州禽畜寄生蜱种类及优势种[J]. 西南师范大学学报(自然科学版),2011,36(1):98-101. DOI:10.13718/j.cnki.xsxb.2011.01.040.Liu LK,Guo JJ. Species of poultry ticks and its dominant species in Guizhou province[J]. J Southwest China Norm Univ(Natl Sci Ed),2011,36(1):98-101. DOI:10.13718/j.cnki.xsxb.2011.01. 040.(in Chinese)
[16] 徐雨,冯明祥,韦登雄,等. 关岭牛多种寄生虫混合感染病例的实验室诊断与治疗[J]. 贵州畜牧兽医,2020,44(5):54-56.Xu Y,Feng MX,Wei DX,et al. Laboratory diagnosis and treatment of mixed infections of multiple parasites in Guanling cattle[J]. Guizhou J Anim Husb Vet Med,2020,44(5):54-56. (in Chinese)
[17] 陈彦希,张胜沾,张海,等. 牛双芽巴贝斯虫、附红细胞体和支原体混合感染的诊治[J]. 黑龙江畜牧兽医,2018(2):118,120,245. DOI:10.13881/j.cnki.hljxmsy.2017.02.0088.Chen YX,Zhang SZ,Zhang H,et al. Diagnosis and treatment of the coinfection of Babesia bigemina,Eperythrozoon and Mycoplasma in cattle[J]. Heilongjiang Anim Sci Vet Med,2018(2):118,120,245. DOI:10.13881/j.cnki.hljxmsy.2017.02.0088.(in Chinese)
[18] 周启泽,张毅. 奶牛双芽巴贝斯虫病的诊治[J]. 中国兽医杂志,2015,51(2):96-97. DOI:10.3969/j.issn.0529-6005.2015. 02.038.Zhou QZ,Zhang Y. Diagnosis and treatment of Babesia bigemina in dairy cows[J]. Chin J Vet Med,2015,51(2):96-97. DOI:10.3969/j.issn.0529-6005.2015.02.038.(in Chinese)
[19] 肖鹏. 微小扇头蜱空间分布及感染病原体流行情况分析[D]. 北京:北京化工大学,2022. DOI:10.26939/d.cnki.gbhgu.2022. 001560.Xiao P. Prediction of spatial distribution and epidemic analysis of pathogens of Rhipicephalus microplus[J]. Beijing:Beijing University of Chemical Technology,2022. DOI:10.26939/d.cnki.gbhgu.2022.001560.(in Chinese)
[20] 吴位珩,杨茂生,廖明,等. 牛双芽巴贝斯虫PCR检测方法的建立及初步应用[J]. 畜牧与兽医,2009,41(9):78-80.Wu WH,Yang MS,Liao M,et al. Establishment and preliminary application of PCR method for detection of Babesia bigemina in cattle[J]. Anim Husb Vet Med,2009,41(9):78-80. (in Chinese)
[21] 李白. 肉牛焦虫病的流行病学、临床症状和防治措施[J]. 现代畜牧科技,2021(2):103-104. DOI:10.19369/j.cnki.2095-9737.2021.02.055.Li B. Epidemiology,clinical symptoms and control measures of pyrococcosis in beef cattle[J]. Mod Anim Husb Sci Technol,2021(2):103-104. DOI:10.19369/j.cnki.2095-9737.2021.02. 055.(in Chinese)
[22] Ma ML,Chen Z,Liu AH,et al. Biological parameters of Rhipicephalus (Boophilus) microplus (Acari:Ixodidae) fed on rabbits,sheep,and cattle[J]. Korean J Parasitol,2016,54(3):301-305. DOI:10.3347/kjp.2016.54.3.301.
[23] 赵国平. 中国蜱类空间分布及其危害预测[D]. 北京:军事科学院,2018.Zhao GP. Spatial distribution of ticks and their hazard prediction in China[D]. Beijing:Academy of Military Sciences,2018. (in Chinese)
[24] Torina A,Agnone A,Sireci G,et al. Characterization of the apical membrane antigen-1 in Italian strains of Babesia bigemina[J]. Transbound Emerg Dis,2010,57(1/2):52-56. DOI:10.1111/j.1865-1682.2010.01118.x.
[25] Ord RL,Lobo CA. Human babesiosis:Pathogens,prevalence,diagnosis,and treatment[J]. Curr Clin Microbiol Rep,2015,2(4):173-181. DOI:10.1007/s40588-015-0025-z.
[26] Zhao GP,Wang YX,Fan ZW,et al. Mapping ticks and tick-borne pathogens in China[J]. Nat Commun,2021,12(1):1075. DOI:10.1038/s41467-021-21375-1.

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