Objective To investigate the insecticide resistance level of Musca domestica in human settlements and surroundings in Hefei, China, and to provide a scientific basis for insecticide use and management as well as fly control. Methods The topical application method was used to determine the resistance levels of M. domestica to insecticides. Probit analysis was performed with SPSS 22.0 software to calculate the toxicity regression equation as well as median lethal dose (LD50) and 95% confidence interval. Results For M. domestica in Hefei, 2021, the LD50 values of dichlorvos, permethrin, deltamethrin, alpha-cypermethrin, beta-cypermethrin, and lambda-cyhalothrin were 0.321 55, 0.775 62, 0.956 54, 0.519 00, 0.542 90, and 0.532 90 μg/♀, respectively; the relative resistance coefficients were 28.21, 133.73, 1 471.60, 112.83, 63.13, and 166.53, respectively, indicating high resistance levels of M. domestica to all the six insecticides. Conclusion M. domestica in Hefei developed high resistance to the six commonly used insecticides, which should be avoided or reduced in use. Other insecticides of high sensitivity or with different mechanisms of action should be used in alternation to delay the development of resistance.
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