上海地区不同生境游离蜱分布调查

曾文博, 李中秋, 魏子昕, 程东慧, 杨丽敏, 方圆, 张仪

doi:10.11853/j.issn.1003.8280.2022.06.022

中国媒介生物学及控制杂志 >

2022 , Vol. 33 >Issue 6: 879 - 887

DOI: https://doi.org/10.11853/j.issn.1003.8280.2022.06.022

调查研究

上海地区不同生境游离蜱分布调查

展开

1. 中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心), 国家卫生健康委员会寄生虫病原与媒介生物学重点实验室, 世界卫生组织热带病合作中心, 国家级热带病国际联合研究中心, 上海 200025;
2. 上海交通大学医学院, 国家热带病研究中心全球健康学院, 上海 200025

曾文博,男,在读硕士,从事医学媒介生物防制研究,E-mail:wenbozenger@163.com

收稿日期: 2022-08-18

网络出版日期: 2022-12-09

基金资助

上海市第五轮公共卫生体系建设三年行动计划重点学科项目（GWV-10.1-XK13）；国家科技基础资源调查专项（2017FY101203）

收起

An investigation of questing ticks collected from different habitats in Shanghai, China

Expand

1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China;
2. School of Global Health, Shanghai Jiao Tong University School of Medicine and Chinese Center for Tropical Diseases Research, Shanghai 200025, China

Received date: 2022-08-18

Online published: 2022-12-09

Supported by

Fifth Round of Three-year Action for Public Health System Construction in Shanghai (No. GWV-10.1-XK13); Special Foundation of Basic Science and Technology Resources Survey of Ministry of Science and Technology of China (No. 2017FY101203)

Fold

摘要

目的调查上海地区不同地点、不同生境游离媒介蜱的种类及分布，为蜱媒疾病防制提供依据。方法采用布旗法，于2021年4－7和9－11月在松江、黄浦、崇明、嘉定、宝山、静安、普陀和闵行区等13个调查点采集植被中的游离蜱；形态鉴定后扩增16S rRNA和细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列，利用获得的序列鉴定蜱种并构建系统发育树。结果共采获游离蜱1 937只，隶属2属3种，分别为血蜱属的褐黄血蜱、长角血蜱和扇头蜱属的微小扇头蜱，分别占采获总数的92.36%、6.66%和0.98%。山坡林地采集褐黄血蜱和微小扇头蜱2种400只，占20.65%；公园/景区采集褐黄血蜱和长角血蜱2种1 341只，占69.23%；农村荒地采集褐黄血蜱1种82只，占4.23%；城市绿地采集褐黄血蜱1种114只，占5.89%。系统进化树显示，采获的同一蜱种位于同一进化分支上。结论上海地区不同生境中游离蜱共有3种，不同生境中蜱的种类和数量分布不同，褐黄血蜱为各类生境中的优势种。

关键词： 蜱; 分布; 16S rRNA基因; 细胞色素C氧化酶亚基Ⅰ基因; 系统发育分析

本文引用格式

曾文博, 李中秋, 魏子昕, 程东慧, 杨丽敏, 方圆, 张仪 . 上海地区不同生境游离蜱分布调查[J]. 中国媒介生物学及控制杂志, 2022 , 33(6) : 879 -887 . DOI: 10.11853/j.issn.1003.8280.2022.06.022

Abstract

Objective To investigate tick species and distribution in different locations and habitats in Shanghai, China, and to provide basic data for the prevention and control of tick-borne diseases. Methods From April to November, except August, in 2021, the drag-flag method was used to collect questing ticks in vegetation at 13 investigation sites in Songjiang, Huangpu, Chongming, Jiading, Baoshan, Jing’an, Putuo, and Minhang districts. After morphological identification, the 16S rRNA and cytochrome c oxidase subunit Ⅰ (COⅠ) gene were amplified to identify the tick species and construct a phylogenetic tree. Results A total of 1 937 questing ticks were collected, which belonged to 3 species from 2 genera, i.e., Haemaphysalis flava (92.36%), H. longicornis (6.66%), and Rhipicephalus microplus (0.98%). Specifically, 400 ticks (20.65%) belonging to H. flava and R. microplus were collected from wooded hillsides; 1 341 (69.23%) belonging to H. flava and H. longicornis were collected from parks and scenic spots; 82 (4.23%) belonging to H. flava were collected from rural wild lands; 114 (5.89%) belonging to H. flava were collected from urban green lands. The phylogenetic tree showed that the collected same tick species were in the same evolutionary branch. Conclusion There are three species of questing ticks in different habitats in Shanghai. The species and number of ticks vary across habitats. H. flava is the dominant species in each type of habitat.

Key words： Tick; Distribution; 16S rRNA gene; COⅠ gene; Phylogenetic analysis

参考文献

[1] Dantas-Torres F,Chomel BB,Otranto D. Ticks and tick-borne diseases:A One Health perspective[J]. Trends Parasitol,2012,28(10):437-446. DOI:10.1016/j.pt.2012.07.003.
[2] 于志军,刘敬泽. 蜱传疾病及其媒介蜱类研究进展[J]. 应用昆虫学报,2015,52(5):1072-1081. DOI:10.7679/j.issn.2095?1353.2015.128. Yu ZJ,Liu JZ. Progress in research on tick-borne diseases and vector ticks[J]. Chin J Appl Entomol,2015,52(5):1072-1081. DOI:10.7679/j.issn.2095?1353.2015.128. (in Chinese)
[3] 张芳. 我国土拉弗氏菌分子流行病学调查研究[D]. 北京:中国人民解放军军事医学科学院,2008. Zhang F. Molecular epidemiological investigation on Francisella tularensis in China[D]. Beijing:Academy of Military Medical Sciences,2008. (in Chinese)
[4] Liu Q,He B,Huang SY,et al. Severe fever with thrombocytopenia syndrome,an emerging tick-borne zoonosis[J]. Lancet Infect Dis,2014,14(8):763-772. DOI:10.1016/s1473-3099(14)70718-2.
[5] 汪金生,龚磊,李贤相,等. 安庆市137例发热伴血小板减少综合征流行病学调查及传播媒介蜱虫监测结果[J]. 职业与健康,2019,35(4):516-520. DOI:10.13329/j.cnki.zyyjk.2019. 0141. Wang JS,Gong L,Li XX,et al. Epidemiological investigation of 137 cases of severe fever with thrombocytopenia syndrome and monitoring results of vehicle ticks in Anqing city[J]. Occup Health,2019,35(4):516-520. DOI:10.13329/j.cnki.zyyjk. 2019.0141.(in Chinese)
[6] Davari B,Alam FN,Nasirian H,et al. Seasonal distribution and faunistic of ticks in the Alashtar county (Lorestan province),Iran[J]. Pan Afr Med J,2017,27:284. DOI:10.11604/pamj. 2017.27.284.10341.
[7] Haut M,Król N,Obiegala A,et al. Under the skin:Ixodes ticks in the subcutaneous tissue of red foxes (Vulpes vulpes) from Germany[J]. Parasit Vectors,2020,13(1):189. DOI:10.1186/s13071-020-04061-x.
[8] 刘洪霞,范明秋,刘曜,等. 2015-2016年上海地区动物体表及其周边环境蜱侵害调查[J]. 上海预防医学,2018,30(8):649-651. DOI:10.19428/j.cnki.sjpm.2018.18784. Liu HX,Fan MQ,Liu Y,et al. Investigation of ticks parasitizing animal body surface and surroundings in Shanghai,2015-2016[J]. Shanghai J Prev Med,2018,30(8):649-651. DOI:10.19428/j.cnki.sjpm.2018.18784.(in Chinese)
[9] 刘琴,危芙蓉,朱丹,等. 上海市犬体表媒介蜱寄生情况初步调查[J]. 中国血吸虫病防治杂志,2012,24(5):537-539,543. DOI:10.16250/j.32.1374.2012.05.012. Liu Q,Wei FR,Zhu D,et al. Investigation of ticks parasitizing on bodies of dogs in Shanghai[J]. Chin J Schisto Control,2012,24(5):537-539,543. DOI:10.16250/j.32.1374.2012.05.012.(in Chinese)
[10] 张维谊,姚佳玲,周珂珂,等. 上海市警犬寄生蜱种鉴定及其携带的原虫及微生物组分析[J]. 中国动物传染病学报,2019,27(6):48-53. Zhang WY,Yao JL,Zhou KK,et al. Analysis on protozoa species and microbial populations of ticks from police dogs in Shanghai,China[J]. Chin J Anim Infect Dis,2019,27(6):48-53. (in Chinese)
[11] 马桢红,陈学良,顾灯安,等. 江苏省苏州地区不同植被类型中游离蜱的调查[J]. 中国媒介生物学及控制杂志,2020,31(1):92-95. DOI:10.11853/j.issn.1003.8280.2020.01.019. Ma ZH,Chen XL,Gu DA,et al. An investigation of questing ticks collected from the habitats with different vegetation types in Suzhou,Jiangsu province,China[J]. Chin J Vector Biol Control,2020,31(1):92-95. DOI:10.11853/j.issn.1003.8280.2020.01. 019.(in Chinese)
[12] 许世远,黄仰松,范安康. 上海市地貌类型与地貌分区[J]. 华东师范大学学报(自然科学版),1986(4):75-82. Xu SY,Huang YS,Fan AK. An analysis on geomorphology typesl and zonation of the Shanghai municipality area[J]. J East China Normal Univ (Nat Sci Ed),1986(4):75-82. (in Chinese)
[13] 田旗. 上海自然植被季相与城市绿化[J]. 园林,2006(2):42-43. Tian Q. Seasonal phase of natural vegetation and urban greening in Shanghai[J]. Garden,2006(2):42-43. (in Chinese)
[14] 李中秋,李兰花,殷会军,等. 长三角城市群蜱媒分布及适生区分析[J]. 中国血吸虫病防治杂志,2021,33(4):365-372. DOI:10.16250/j.32.1374.2021068. Li ZQ,Li LH,Yin HJ,et al. Distribution and suitable habitats of ticks in the Yangtze River Delta urban agglomeration[J]. Chin J Schisto Control,2021,33(4):365-372. DOI:10.16250/j.32. 1374.2021068.(in Chinese)
[15] Black Ⅳ WC,Piesman J. Phylogeny of hard- and soft-tick taxa (Acari:Ixodida) based on mitochondrial 16S rDNA sequences[J]. Proc Natl Acad Sci USA,1994,91(21):10034-10038. DOI:10.1073/pnas.91.21.10034.
[16] Chitimia L,Lin RQ,Cosoroaba I,et al. Genetic characterization of ticks from southwestern Romania by sequences of mitochondrial cox1 and nad5 genes[J]. Exp Appl Acarol,2010,52(3):305-311. DOI:10.1007/s10493-010-9365-9.
[17] 魏子昕,方圆,张仪. 上海市蜱种类、分布及其携带病原[J]. 中国媒介生物学及控制杂志,2022,33(1):120-124. DOI:10.11853/j.issn.1003.8280.2022.01.022. Wei ZX,Fang Y,Zhang Y. Ticks species,distribution and pathogens in Shanghai,China[J]. Chin J Vector Biol Control,2022,33(1):120-124. DOI:10.11853/j.issn.1003.8280.2022. 01.022.(in Chinese)
[18] 陈泽,杨晓军. 蜱的系统分类学[M]. 北京:科学出版社,2021:653. Chen Z,Yang XJ. Systematics of ticks[M]. Beijing:Science Press,2021:653. (in Chinese)
[19] Fang LZ,Lei SC,Yan ZJ,et al. Detection of multiple intracellular bacterial pathogens in Haemaphysalis flava ticks collected from hedgehogs in central China[J]. Pathogens,2021,10(2):115. DOI:10.3390/pathogens10020115.
[20] Zhao L,Li J,Cui XM,et al. Distribution of Haemaphysalis longicornis and associated pathogens:Analysis of pooled data from a China field survey and global published data[J]. Lancet Planet Health,2020,4(8):E320-E329. DOI:10.1016/s2542-5196(20)30145-5.
[21] Sumrandee C,Baimai V,Trinachartvanit W,et al. Molecular detection of Rickettsia,Anaplasma,Coxiella and Francisella bacteria in ticks collected from Artiodactyla in Thailand[J]. Ticks Tick-Borne Dis,2016,7(5):678-689. DOI:10.1016/j.ttbdis.2016.02.015.
[22] Fang LQ,Liu K,Li XL,et al. Emerging tick-borne infections in mainland China:An increasing public health threat[J]. Lancet Infect Dis,2015,15(12):1467-1479. DOI:10.1016/s1473-3099(15)00177-2.
[23] Wang ZD,Wang B,Wei F,et al. A new segmented virus associated with human febrile illness in China[J]. N Engl J Med,2019,380(22):2116-2125. DOI:10.1056/NEJMoa1805068.
[24] Liu SL,Chai CL,Wang CM,et al. Systematic review of severe fever with thrombocytopenia syndrome:Virology,epidemiology,and clinical characteristics[J]. Rev Med Virol,2014,24(2):90-102. DOI:10.1002/rmv.1776.
[25] Kodama F,Yamaguchi H,Park E,et al. A novel nairovirus associated with acute febrile illness in Hokkaido,Japan[J]. Nat Commun,2021,12(1):5539. DOI:10.1038/s41467-021-25857-0.
[26] Xu BL,Liu LC,Huang XY,et al. Metagenomic analysis of fever,thrombocytopenia and leukopenia syndrome (FTLS) in Henan province,China:Discovery of a new bunyavirus[J]. PLoS Pathog,2011,7(11):e1002369. DOI:10.1371/journal.ppat.1002369.
[27] Batool M,Blazier JC,Rogovska YV,et al. Metagenomic analysis of individually analyzed ticks from Eastern Europe demonstrates regional and sex-dependent differences in the microbiota of Ixodes ricinus[J]. Ticks Tick-Borne Dis,2021,12(5):101768. DOI:10.1016/j.ttbdis.2021.101768.
[28] 沈钟平,梁萍,何金海. 上海城市热岛的精细结构气候特征分析[J]. 大气科学学报,2017,40(3):369-378. DOI:10.13878/j.cnki.dqkxxb.20160922001. Shen ZP,Liang P,He JH. Analysis on the climatic characteristics of the fine structure of the urban heat island in Shanghai[J]. Trans Atmos Sci,2017,40(3):369-378. DOI:10.13878/j.cnki.dqkxxb.20160922001.(in Chinese)
[29] 刘琴,张仪,方圆,等. 基于16S rDNA和COⅠ基因的3种血蜱分子生物学鉴定[J]. 国际医学寄生虫病杂志,2015,42(3):146-151. Liu Q,Zhang Y,Fang Y,et al. Identification of Haemaphysalis longicornis,H. flava and H. campanulata based on molecular markers of 16S rDNA and COⅠ gene[J]. Int J Med Parasit Dis,2015,42(3):146-151. (in Chinese)

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献