目的 掌握2020年河北省承德市家蝇对不同类型杀虫剂的抗药性水平，与近10年承德市家蝇的抗药性水平数据比较，为指导家蝇防治提出科学依据。方法 2020年在承德市的农贸市场、餐饮外环境、绿化带、城市居民区及农村自然村5种生境利用网捕法捕捉家蝇，经饲养室繁殖1～2代后，采用微量点滴法测定其对毒死蜱、高效氯氰菊酯和溴氰菊酯3种常用杀虫剂的半数致死量（LD50），并利用实验室结果所得数据采用DPS软件进行统计学分析，结合近10年的家蝇抗药性水平数据进行对比分析。结果 2020年承德市家蝇对毒死蜱的抗药性水平为低抗，抗药性倍数为6.51倍；对高效氯氰菊酯和溴氰菊酯的抗药性水平均为高抗，抗药性倍数分别为90.48和370.00倍。通过与近10年抗药性数据进行对比分析发现，承德市家蝇对有机磷类杀虫剂始终保持着中低抗水平，对拟除虫菊酯类杀虫剂抗药性总体水平呈现升高趋势。结论 河北省承德市家蝇近10年来对不同类型的杀虫剂均有不同程度的抗药性，因此应及时调整并精准使用杀虫剂，从而科学有效抑制和延缓家蝇对杀虫剂抗药性的发展。
Objective To investigate the resistance levels of Musca domestica to different types of insecticides in Chengde, Hebei province, China in 2020, to compare the resistance levels with those in the past ten years, and to provide a scientific basis for guiding M. domestica control. Methods The netting method was used to catch M. domestica in five types of habitats, including farmers' markets, restaurants, green belts, urban residential areas, and rural villages, in Chengde in 2020. After reproduction for 1 to 2 generations in the rearing room, the micro-drop method was used to determine the median lethal dose of three commonly used insecticides, i.e., chlorpyrifos, beta-cypermethrin, and deltamethrin. Data Processing System software was used to statistically analyze the laboratory results. A comparative analysis was performed on the resistance levels of M. domestica between the year 2020 and the past ten years. Results In 2020, M. domestica in Chengde had a low resistance level to chlorpyrifos, with a resistance ratio of 6.51, and had high resistance levels to beta-cypermethrin and deltamethrin, with resistance ratios of 90.48 and 370.00. The comparative analysis on the resistance data between the year 2020 and the past ten years revealed that M. domestica in Chengde had been having moderate or low resistance levels to organophosphorus insecticides, and had a rising trend in the overall resistance level to pyrethroid insecticides. Conclusion M. domestica in Chengde, Hebei province has developed different levels of resistance to different types of insecticides in the past ten years. Therefore, the insecticides should be reasonably and accurately used to scientifically and effectively inhibit and delay the development of M. domestica resistance.
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