塞罕坝自然保护区蜱媒斑点热群立克次体检测及遗传进化分析

高杨, 许士奇, 王丽娜, 郭文平, 刘铭

doi:10.11853/j.issn.1003.8280.2022.02.016

中国媒介生物学及控制杂志 >

2022 , Vol. 33 >Issue 2: 252 - 257

DOI: https://doi.org/10.11853/j.issn.1003.8280.2022.02.016

媒介生物传染病

塞罕坝自然保护区蜱媒斑点热群立克次体检测及遗传进化分析

展开

承德医学院基础医学院病原生物学教研室, 河北承德 067000

高杨,女,硕士,讲师,主要从事病原生物学研究,E-mail:326194867@qq.com

收稿日期: 2021-10-19

网络出版日期: 2022-05-09

基金资助

河北省教育厅青年基金（QN2018027）

收起

Detection and phylogenetic analysis of spotted fever group rickettsiae in ticks in Saihanba National Nature Reserve, China

Expand

School of Basic Medical Science/Department of Biomedical Engineering, Chengde Medical University, Chengde, Hebei 067000, China

Received date: 2021-10-19

Online published: 2022-05-09

Supported by

Youth Foundation of Hebei Provincial Department of Education (No. QN2018027)

Fold

摘要

目的调查塞罕坝自然保护区蜱媒斑点热群立克次体（SFGR）的感染情况，分析该地区蜱媒体内SFGR种类并进行同源性分析，为防控蜱媒疾病提供理论依据。方法在塞罕坝自然保护区内采用布旗法收集游离蜱并提取DNA，采用PCR技术对SFGR外膜蛋白A基因（ompA）和柠檬酸合成酶A基因（gltA）进行扩增，对阳性样本进行序列测定，同时构建分子系统进化树进行分析。结果在塞罕坝自然保护区内共采集游离蜱919只，其中全沟硬蜱546只、嗜群血蜱242只、森林革蜱131只。测得SFGR阳性蜱72只，总阳性率为7.8%，其中全沟硬蜱阳性率为6.2%（34/546），嗜群血蜱为9.5%（23/242），森林革蜱为11.5%（15/131）；不同蜱种SFGR感染率差异无统计学意义（χ²=1.261，P=0.263）。分别对ompA和gltA基因进行序列比对，发现该地区存在劳氏立克次体与新塔拉塞维奇立克次体2种SFGR。分子系统进化树（ompA和gltA基因）显示，森林革蜱携带的SFGR与GenBank中劳氏立克次体（AH015610、DQ365804）聚为同一分支；全沟硬蜱和嗜群血蜱携带的SFGR与GenBank中新塔拉塞维奇立克次体（MT667416、MT66740）亲缘关系最近，处于同一分支。结论塞罕坝自然保护区内存在劳氏立克次体和新塔拉塞维奇立克次体，因此对当地蜱以及蜱媒疾病应加强防控，以免对当地人群健康造成威胁。

关键词： 蜱; 斑点热群立克次体; 塞罕坝; 序列分析

本文引用格式

高杨, 许士奇, 王丽娜, 郭文平, 刘铭 . 塞罕坝自然保护区蜱媒斑点热群立克次体检测及遗传进化分析[J]. 中国媒介生物学及控制杂志, 2022 , 33(2) : 252 -257 . DOI: 10.11853/j.issn.1003.8280.2022.02.016

Abstract

Objective To investigate the status of spotted fever group rickettsiae (SFGR) infection and SFGR species in ticks in Saihanba National Nature Reserve of Hebei province, China, to perform homology analysis for SFGR in ticks, and to provide a theoretical basis for the prevention and control of tick-borne diseases. Methods Free-living ticks were collected by cloth dragging in Saihanba National Nature Reserve. After DNA extraction, PCR was used to amplify the outer membrane protein A gene (ompA) and citrate synthase gene (gltA) of SFGR, and sequencing was performed on the positive samples, followed by molecular phylogenetic analysis. Results A total of 919 free-living ticks were collected in Saihanba National Nature Reserve, including 546 Ixodes persulcatus ticks, 242 Haemaphysalis concinna ticks, and 131 Dermacentor silvarum ticks. There were 72 SFGR-positive ticks, with an overall positive rate of 7.8%. The positive rate was 6.2% (34/546) in I. persulcatus, 9.5% (23/242) in H. concinna, and 11.5% (15/131) in D. silvarum. There was no statistical difference in SFGR infection rate between different tick species (χ²=1.261, P=0.263). Sequence alignment of the ompA and gltA genes revealed that two species of SFGR were present in the area, being Rickettsia raoultii and Candidatus R. tarasevichiae. The molecular phylogenetic analyses of the two genes showed that SFGR carried by D. silvarum was clustered with R. raoultii (AH015610 and DQ365804) in GenBank, and the rickettsiae carried by I. persulcatus and H. concinna were clustered with Candidatus R. tarasevichiae (MT667416 and MT66740) in GenBank. Conclusion R. raoultii and Candidatus R. tarasevichiae are present in Saihanba National Nature Reserve. In order to avoid threatening public health, it is necessary to strengthen the prevention and control of ticks and tick-borne diseases in the area.

Key words： Tick; Spotted fever group rickettsiae; Saihanba; Sequence analysis

参考文献

[1] Merhej V, Angelakis E, Socolovschi C, et al. Genotyping, evolution and epidemiological findings of Rickettsia species[J]. Infect Genet Evol, 2014, 25:122-137. DOI:10.1016/j.meegid. 2014.03.014.
[2] Vanegas A, Keller C, Krüger A, et al. Molecular detection of spotted fever group rickettsiae in ticks from Cameroon[J]. Ticks Tick-Borne Dis, 2018, 9(4):1049-1056. DOI:10.1016/j.ttbdis. 2018.03.022.
[3] Fang LQ, Liu K, Li XL, et al. Emerging tick-borne infections in mainland China:An increasing public health threat[J]. Lancet Infect Dis, 2015, 15(12):1467-1479. DOI:10.1016/S1473-3099(15)00177-2.
[4] Parola P, Paddock CD, Socolovschi C, et al. Update on tick-borne rickettsioses around the world:A geographic approach[J]. Clin Microbiol Rev, 2014, 27(1):657-702. DOI:10.1128/CMR. 00104-13.
[5] Wei QQ, Guo LP, Wang AD, et al. The first detection of Rickettsia aeschlimannii and R. massiliae in Rhipicephalus turanicus ticks, in northwest China[J]. Parasit Vectors, 2015, 8(1):631. DOI:10.1186/s13071-015-1242-2.
[6] Yang JF, Tian ZC, Liu ZJ, et al. Novel spotted fever group rickettsiae in Haemaphysalis qinghaiensis ticks from Gansu, Northwest China[J]. Parasit Vectors, 2016, 9(1):146. DOI:10.1186/s13071-016-1423-7.
[7] Li H, Cui XM, Cui N, et al. Human infection with novel spotted fever group Rickettsia genotype, China, 2015[J]. Emerg Infect Dis, 2016, 22(12):2153-2156. DOI:10.3201/eid2212.160962.
[8] 王卓, 王建伟, 于淼, 等. 东北地区蜱传斑点热群立克次体的分子流行病学研究[J]. 中国媒介生物学及控制杂志, 2018, 29(4):344-347. DOI:10.11853/j.issn.1003.8280.2018.04.005. Wang Z, Wang JW, Yu M, et al. Molecular epidemiological studies on spotted fever group Rickettsia in ticks from Northeastern China[J]. Chin J Vector Biol Control, 2018, 29(4):344-347. DOI:10.11853/j.issn.1003.8280.2018.04.005.(in Chinese)
[9] 张艳凯, 刘敬泽. 蜱类病原体和共生菌多样性及其作用[J]. 应用昆虫学报, 2019, 56(1):1-11. DOI:10.7679/j.issn.2095-1353.2019.001. Zhang YK, Liu JZ. Diversity, and roles, of pathogens and symbionts in ticks[J]. Chin J Appl Entomol, 2019, 56(1):1-11. DOI:10.7679/j.issn.2095-1353.2019.001.(in Chinese)
[10] 周磊, 汤芳, 栾进, 等. 内蒙古奇乾地区蜱携带斑点热立克次体的调查[J]. 中国国境卫生检疫杂志, 2017, 40(2):96-99. DOI:10.16408/j.1004-9770.2017.02.006. Zhou L, Tang F, Luan J, et al. Investigation on spotted fever group rickettsiae in ticks collected from Qiqian areas of Inner Mongolia[J]. Chin J Front Health Quarant, 2017, 40(2):96-99. DOI:10.16408/j.1004-9770.2017.02.006.(in Chinese)
[11] 冯立, 王卓, 杨军, 等. 黑龙江逊克地区森林革蜱斑点热立克次体DNA的检测[J]. 中国人兽共患病学报, 2014, 30(10):1020-1023. DOI:10.3969/cjz.j.issn.1002-2694.2014.10.007. Feng L, Wang Z, Yang J, et al. Molecular detection of spotted fever group Rickettsia in Dermacentor silvarum from the Xunke area, China[J]. Chin J Zoonoses, 2014, 30(10):1020-1023. DOI:10.3969/cjz.j.issn.1002-2694.2014.10.007.(in Chinese)
[12] Parola P, Paddock CD, Raoult D. Tick-borne rickettsioses around the world:Emerging diseases challenging old concepts[J]. Clin Microbiol Rev, 2005, 18(4):719-756. DOI:10.1128/CMR.18. 4.719-756.2005.
[13] 于志军, 刘敬泽. 蜱传疾病及其媒介蜱类研究进展[J]. 应用昆虫学报, 2015, 52(5):1072-1087. DOI:10.7679/j.issn.2095-1353.2015.128. Yu ZJ, Liu JZ. Progress in research on tick-borne diseases and vector ticks[J]. Chin J Appl Entomol, 2015, 52(5):1072-1087. DOI:10.7679/j.issn.2095-1353.2015.128.(in Chinese)
[14] 李思思, 张晓雨, 张艳凯, 等. 内蒙古地区全沟硬蜱细菌群落结构及多样性分析[J]. 中国媒介生物学及控制杂志, 2019, 30(6):607-612. DOI:10.11853/j.issn.1003.8280.2019.06.003. Li SS, Zhang XY, Zhang YK, et al. An analysis of bacterial community composition and diversity in Ixodes persulcatus in Inner Mongolia, China[J]. Chin J Vector Biol Control, 2019, 30(6):607-612. DOI:10.11853/j.issn.1003.8280.2019.06.003.(in Chinese)
[15] 高杨, 许士奇, 李丽静, 等. 塞罕坝自然保护区硬蜱调查及分子鉴定[J]. 中国病原生物学杂志, 2019, 14(9):1054-1057, 1075. DOI:10.13350/j.cjpb.190914. Gao Y, Xu SQ, Li LJ, et al. Molecular identification of ixodid ticks collected from Saihanba in Hebei province[J]. J Parasit Biol, 2019, 14(9):1054-1057, 1075. DOI:10.13350/j.cjpb. 190914.(in Chinese)
[16] 周明浩, 陈红娜. 我国新发蜱媒病原体研究概述[J]. 中华卫生杀虫药械, 2019, 25(3):193-198. DOI:10.19821/j.1671-2781.2019.03.001. Zhou MH, Chen HN. A review on emerging tick-borne pathogens in China[J]. Chin J Hyg Insect Equip, 2019, 25(3):193-198. DOI:10.19821/j.1671-2781.2019.03.001.(in Chinese)
[17] 乌兰图雅, 殷旭红, 崔云虹, 等. 内蒙古中西部草原蜱媒病原体多样性及基因型分析[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(1):27-34, 42. DOI:10.12140/j.issn.1000-7423.2021. 01.004. Wulantuya, Yin XH, Cui YH, et al. Diversity and genotype analysis of tick-borne pathogens in grasslands in the central and western part of Inner Mongolia[J]. Chin J Parasitol Parasit Dis, 2021, 39(1):27-34, 42. DOI:10.12140/j.issn.1000-7423.2021. 01.004.(in Chinese)
[18] 闻静, 焦丹, 鞠文东, 等. 新发蜱传病原体:劳氏立克次体的研究现状[J]. 中国人兽共患病学报, 2015, 31(4):361-364. DOI:10.3969/cjz.j.issn.1002-2694.2015.04.015. Wen J, Jiao D, Ju WD, et al. Emerging tick-borne pathogen:The research status on Rickettsia raoultii[J]. Chin J Zoonoses, 2015, 31(4):361-364. DOI:10.3969/cjz.j.issn.1002-2694.2015.04. 015.(in Chinese)
[19] 桂峥, 于晶峰, 木兰. 内蒙古部分地区草原革蜱携带斑点热群立克次体DNA检测及其基因型分布[J]. 吉林大学学报(医学版), 2021, 47(1):210-215. DOI:10.13481/j.1671-587Ⅹ. 20210129. Gui Z, Yu JF, Mu L. Detection of DNA in spotted fever group Rickettsia carried by Dermacentor nuttalli in partial areas of Inner Mongolia and its distribution of genotypes[J]. J Jilin Univ(Med Ed)2021, 47(1):210-215. DOI:10.13481/j.1671⁃587Ⅹ. 20210129. (in Chinese)
[20] 姜理平, 孟真, 崔清荣, 等. 浙江省蜱标本中斑点热群立克次体rOmpA和gltA基因检测[J]. 中国媒介生物学及控制杂志, 2010, 21(4):350-352. Jiang LP, Meng Z, Cui QR, et al. Detection of rOmpA and gltA genes of spotted fever group rickettsiae from tick specimens in Zhejiang province[J]. Chin J Vector Biol Control, 2010, 21(4):350-352. (in Chinese)
[21] 徐广, 方庆权, Keirans JE, et al. 分子系统进化关系分析的一种新方法:贝叶斯法在硬蜱属中的应用[J]. 动物学报, 2003, 49(3):380-388. DOI:10.3969/j.issn.1674-5507.2003.03.013. Xu G, Fang QQ, Keirans JE, et al. Application of Bayesian method for inferring phylogenetic relationships of ticks within the genus Ixodes[J]. Acta Zool Sin, 2003, 49(3):380-388. DOI:10.3969/j.issn.1674-5507.2003.03.013.(in Chinese)

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献