Objective To investigate the resistance of Musca domestica to commonly used insecticides in Baiyin city, Gansu province, China, and to provide evidence for rational use of insecticides, and scientific and sustainable vector control. Methods The WHO micro-drop method was used to determine the resistance to commonly used insecticides of female M. domestica 3-5 days after emergence. Results M. domestica in both Baiyin district and Pingchuan district of Baiyin developed extremely high resistance to DDVP (202.33 and 268.33 times, respectively) and high resistance to pyrethroid insecticides. The median lethal dose (LD50) values of beta-cypermethrin, permethrin, and deltamethrin for M. domestica were 0.621, 0.296, and 0.187 μg/fly in Baiyin district, respectively, and 0.827, 0.497, 0.198 μg/fly in Pingchuan district, respectively. The LD50 of propoxur in Baiyin district and Pingchuan district were 1.467 and 2.183 μg/fly, respectively, which belong to a low resistance level. The resistance levels to the five insecticides were significantly higher in Baiyin district and Pingchuan district (succeeded in creating the national healthy cities) than in Jingyuan county (not applying for the national healthy city; all P<0.05). Conclusion M. domestica in Baiyin city has developed resistance to organophosphate and pyrethroid insecticides. It is recommend to conduct long-term and continuous vector resistance surveillance to guide scientific and rational use of insecticides, and adopt comprehensive sustainable control strategies focusing on environmental governance and physical control.
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