目的 了解河南省南阳市城区德国小蠊对5种常用杀虫剂的抗药性，为科学、合理用药提供理论依据。方法 从南阳市的农贸市场、餐饮场所、宾馆、超市及医院采集德国小蠊野外种群带回实验室饲养至F1代，采用世界卫生组织推荐的药膜法，测定杀虫剂的半数击倒时间、击倒率及抗性倍数，评价其抗药性情况。结果 南阳市德国小蠊野外种群对溴氰菊酯、高效氯氰菊酯、敌敌畏、双硫磷、仲丁威产生了不同程度的抗药性，抗性倍数分别为12.03、2.14、4.18、247.23和3.85倍。结论 南阳市德国小蠊野外种群对溴氰菊酯和双硫磷抗性较高，建议采用综合防制方法、合理用药，抑制和延缓德国小蠊抗药性的发展。
Objective To investigate the resistance of Blattella germanica to five commonly used insecticides in the urban area of Nanyang, Henan province, China, and to provide a basis for rational use of insecticides in B. germanica control. Methods The field population of B. germanica was collected from farmers’ markets, restaurants, hotels, supermarkets, and hospitals in Nanyang, and brought back to the laboratory for rearing until the F1 generation. The median knockdown time, knockdown rate, and resistance ratio of the insecticides were determined by the residual film method recommended by WHO, and their resistance was evaluated. Results The field population of B. germanica in Nanyang developed varying degrees of resistance to deltamethrin, beta-cypermethrin, DDVP, temephos, and fenobucarb, with resistance ratios of 12.03, 2.14, 4.18, 247.23, and 3.85, respectively. Conclusion The field population of B. germanica in Nanyang has high resistance to deltamethrin and temephos. Comprehensive control methods and rational use of insecticides are recommended to inhibit and delay the development of insecticide resistance of B. germanica.
[1] 白淑萍, 孙晨熹, 李培羽, 等. 德国小蠊对常用杀虫剂的抗性监测[J]. 中华卫生杀虫药械, 2007, 13(2):197-200. DOI:10.3969/j.issn.1671-2781.2007.03.012.Bai SP, Sun CX, Li PY, et al. Resistance surveillance of Blattella germanica to normal insecticides[J]. Chin J Hyg Insect Equip, 2007, 13(2):197-200. DOI:10.3969/j.issn.1671-2781.2007.03.012.
[2] 涂涛田, 季恒青, 陶晓颖, 等. 重庆市德国小蠊对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2017, 28(3):226-229. DOI:10.11853/j.issn.1003.8280.2017.03.007.Tu TT, Ji HQ, Tao XY, et al. Resistance of Blattella germanica to commonly used insecticides in Chongqing[J]. Chin J Vector Biol Control, 2017, 28(3):226-229. DOI:10.11853/j.issn.1003.8280.2017.03.007.
[3] Lee LC, Lee CY. Insecticide resistance profiles and possible underlying mechanisms in German cockroaches, Blattella germanica(Linnaeus)(Dictyoptera:Blattellidae) from Peninsular Malaysia[J]. Med Entomol Zool, 2004, 55(2):77-93. DOI:10.7601/mez.55.77_1.
[4] 邹志辉, 韦薇, 林琳, 等. 深圳市龙岗区德国小蠊自然种群对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2012, 23(2):158-159.Zou ZH, Wei W, Lin L, et al. The investigation on the resistance of Blattella germanica to insecticides in Longgang district of Shenzhen[J]. Chin J Vector Biol Control, 2012, 23(2):158-159.
[5] 谭梁飞, 胡梦佳, 熊进峰, 等. 湖北省德国小蠊对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2018, 29(2):165-167. DOI:10.11853/j.issn.1003.8280.2018.02.012.Tan LF, Hu MJ, Xiong JF, et al. Resistance of Blattella germanica to commonly used insecticides in Hubei province[J]. Chin J Vector Biol Control, 2018, 29(2):165-167. DOI:10.11853/j.issn.1003.8280.2018.02.012.
[6] 邓泽静, 林献丹, 倪朝荣, 等. 德国小蠊对5种常用杀虫剂的抗药性分析[J]. 预防医学, 2019, 31(1):77-79. DOI:10.19485/j.cnki.issn2096-5087.2019.01.020.Deng ZJ, Lin XD, Ni CR, et al. Resistance of Blattella germanica to five kinds of insecticides[J]. Prev Med, 2019, 31(1):77-79. DOI:10.19485/j.cnki.issn2096-5087.2019.01.020.
[7] 马红梅, 李紫粉, 柳小青, 等. 南昌市德国小蠊2006－2016年抗药性发展趋势研究[J]. 中国媒介生物学及控制杂志, 2017, 28(5):454-457. DOI:10.11853/j.issn.1003.8280.2017.05.011.Ma HM, Li ZF, Liu XQ, et al. The resistance dynamics of Blattella germanica in Nanchang city from 2006 to 2016[J]. Chin J Vector Biol Control, 2017, 28(5):454-457. DOI:10.11853/j.issn.1003.8280.2017.05.011.
[8] 孙俊, 杨维芳, 徐燕. 我国蜚蠊及其防治研究概述[J]. 中国媒介生物学及控制杂志, 2009, 20(4):275-280.Sun J, Yang WF, Xu Y. A brief review of cockroach control in China[J]. Chin J Vector Biol Control, 2009, 20(4):275-280.
[9] 刘曜, 刘洪霞, 冷培恩, 等. 4种不同有效成分杀蟑胶饵对德国小蠊的实验室灭效研究[J]. 中国媒介生物学及控制杂志, 2020, 31(5):559-564. DOI:10.11853/j.issn.1003.8280.2020.05.012.Liu Y, Liu HX, Leng PE, et al. Laboratory efficacy against Blattella germanica of four cockroach-killing gel baits containing different effective constituents[J]. Chin J Vector Biol Control, 2020, 31(5):559-564. DOI:10.11853/j.issn.1003.8280.2020.05.012.
[10] 魏绪强, 周小洁. 北京市不同城区德国小蠊对高效氯氰菊酯的抗药性发展动态研究[J]. 中国媒介生物学及控制杂志, 2018, 29(1):27-29. DOI:10.11853/j.issn.1003.8280.2018.01.007.Wei XQ, Zhou XJ. Study on insecticide resistance dynamics to beta-cypermethrin in Blattella germanica collected from different districts, Beijing[J]. Chin J Vector Biol Control, 2018, 29(1):27-29. DOI:10.11853/j.issn.1003.8280.2018.01.007.
