贵州省部分地区2019年蚊虫及蚊媒病毒调查研究

闫冬明, 王文周, 王雪霜, 刘强, 吴兴彬, 赵宁, 梁文琴, 周敬祝, 王丹, 王君, 刘起勇

doi:10.11853/j.issn.1003.8280.2021.04.007

中国媒介生物学及控制杂志 >

2021 , Vol. 32 >Issue 4: 422 - 427

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.04.007

媒介生物病原学监测专题

贵州省部分地区2019年蚊虫及蚊媒病毒调查研究

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1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, WHO媒介生物监测与管理合作中心, 北京 102206;
2. 历城区疾病预防控制中心, 山东济南 250100;
3. 贵州省疾病预防控制中心, 贵州贵阳 550004

闫冬明,男,硕士,主要从事病媒生物与虫媒病研究,E-mail:yandongmingcool@163.com

收稿日期: 2021-03-08

网络出版日期: 2021-08-20

基金资助

中国疾病预防控制中心传染病预防控制所公共卫生突发应急反应机制运行项目（131031102000180007）；传染病预防控制国家重点实验室自主研究课题（2018SKLID101）

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Investigation of mosquitoes and mosquito-borne viruses in some regions of Guizhou province, China, 2019

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1. State Key Laboratory of Infectious Disease Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Licheng Center for Disease Control and Prevention, Ji'nan, Shandong 250100, China;
3. Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China

Received date: 2021-03-08

Online published: 2021-08-20

Supported by

Supported by the Emergency Response Mechanism Operation Program, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (No. 131031102000180007) and Independent Research Project of the State Key Laboratory of Infectious Disease Prevention and Control (No. 2018SKLID101)

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摘要

目的调查贵州省部分地区蚊虫种类及蚊媒病毒分布，为该地区蚊媒传染病防控提供依据。方法用诱蚊灯采集蚊虫，将蚊虫分类鉴定后，用分子生物学方法检测蚊虫携带病毒种类，通过生物信息学软件构建毒株系统发育树。结果 2019年6月在贵州省黎平县中潮镇和乌当区羊昌镇共采集到蚊虫3属9种共27 169只，其中中华按蚊为优势蚊种，占捕获总数的50.47%（13 712/27 169）；其次是三带喙库蚊，占45.80%（12 444/27 169）；其他蚊虫最少，占0.26%（70/27 169）。从黎平县中潮镇的蚊虫标本LK6110中检出1株病毒，经分子生物学鉴定，该病毒株属于基因Ⅲ型流行性乙型脑炎病毒（JEV）。结论本次贵州省调查地区的优势蚊种各不相同，羊昌镇以中华按蚊为主，中潮镇以三带喙库蚊为主，并在黎平县中潮镇三带喙库蚊中检出JEV Ⅲ型病毒。

关键词： 蚊虫; 流行性乙型脑炎; 贵州省

本文引用格式

闫冬明, 王文周, 王雪霜, 刘强, 吴兴彬, 赵宁, 梁文琴, 周敬祝, 王丹, 王君, 刘起勇 . 贵州省部分地区2019年蚊虫及蚊媒病毒调查研究[J]. 中国媒介生物学及控制杂志, 2021 , 32(4) : 422 -427 . DOI: 10.11853/j.issn.1003.8280.2021.04.007

Abstract

Objective To investigate the mosquito species and the distribution of mosquito-borne viruses in some regions of Guizhou province, China, and to provide a basis for the prevention and control of mosquito-borne diseases in this area. Methods Mosquitoes collected with trapping lamps were identified, mosquito-borne viruses were identified by molecular biological methods, and a phylogenetic tree of virus strains was constructed using bioinformatics software. Results A total of 27 169 mosquitoes (9 species, 3 genera) were collected in Zhongchao town of Liping county and Yangchang town of Wudang district (Guiyang city) of Guizhou province in June 2019. The dominant species was Anopheles sinensis (13 712/27 169,50.47%), followed by Culex tritaeniorhynchus (12 444/27 169,45.80%) and other species (70/27 169,0.26%). A virus strain isolated from a mosquito sample (LK6110) from Zhongchao town was identified as Japanese encephalitis virus genotype Ⅲ (JEV Ⅲ). Conclusion In this investigation, Yangchang town was dominated by An. sinensis and Zhongchao town was dominated by Cx. tritaeniorhynchus, and JEV Ⅲ was isolated from Cx. tritaeniorhynchus in Zhongchao town.

Key words： Mosquito; Japanese encephalitis; Guizhou province

参考文献

[1] Nunes MRT, Palacios G, Nunes KNB, et al. Evaluation of two molecular methods for the detection of Yellow fever virus genome[J]. J Virol Methods, 2011, 174(1/2):29-34. DOI:10.1016/j.jviromet.2011.02.025.
[2] 郑学礼. 我国蚊媒研究概况[J]. 中国病原生物学杂志, 2014, 9(2):183-187. DOI:10.13350/j.cjpb.140222.Zheng XL. Advances in research on mosquitoes in China[J]. Chin J Path Biol, 2014, 9(2):183-187. DOI:10.13350/j.cjpb. 140222.
[3] 刘起勇. 新时代媒介生物传染病形势及防控对策[J]. 中国媒介生物学及控制杂志, 2019, 30(1):1-6, 11. DOI:10.11853/j.issn.1003.8280.2019.01.001.Liu QY. Epidemic profile of vector-borne diseases and vector control strategies in the new era[J]. Chin J Vector Biol Control, 2019, 30(1):1-6, 11. DOI:10.11853/j.issn.1003.8280.2019.01.001.
[4] 刘起勇, 甄天民. 媒介生物综合治理全球策略框架[J]. 中国媒介生物学及控制杂志, 2006, 17(2):Ⅰ-Ⅲ. DOI:10.3969/j.issn.1003-4692.2006.02.001.Liu QY, Zhen TM. Global strategic framework for integrated vector management[J]. Chin J Vector Biol Control, 2006, 17(2):Ⅰ-Ⅲ. DOI:10.3969/j.issn.1003-4692.2006.02.001.
[5] 刘起勇. 媒介生物控制面临的挑战与媒介生物可持续控制策略[J]. 中华流行病学杂志, 2012, 33(1):1-8. DOI:10.3760/cma.j.issn.0254-6450.2012.01.001.Liu QY. Challenge to vector control and sustainable vector management strategy[J]. Chin J Epidemiol, 2012, 33(1):1-8. DOI:10.3760/cma.j.issn.0254-6450.2012.01.001.
[6] 朱临, 孙立新, 叶松, 等. 蚊媒病毒检测技术研究综述[J]. 淮南职业技术学院学报, 2011, 11(3):71-73. DOI:10.3969/j.issn.1671-4733.2011.03.024.Zhu L, Sun LX, Ye S, et al. Reviews on the detection technology of mosquito-borne virus[J]. J Huainan Vocat Technical College, 2011, 11(3):71-73. DOI:10.3969/j.issn.1671-4733.2011.03.024.
[7] 叶子, 黄应雄, 蒋鹏, 等. 2014年广州地区158例登革热住院患者临床特征分析[J]. 中华危重病急救医学, 2015, 27(4):300-305. DOI:10.3760/cma.j.issn.2095-4352.2015.04.015.Ye Z, Huang YX, Jiang P, et al. Analysis of clinical characteristic of 158 inpatients with dengue fever in Guangzhou area during the 2014 epidemic[J]. Chin Cri Care Med, 2015, 27(4):300-305. DOI:10.3760/cma.j.issn.2095-4352.2015.04.015.
[8] 李铭华, 付士红, 冯云, 等. 贵州省不同地区2008年虫媒病毒调查[J]. 中国媒介生物学及控制杂志, 2012, 23(5):417-420.Li MH, Fu SH, Feng Y, et al. Investigation of arboviruses in different regions of Guizhou province, China in 2008[J]. Chin J Vector Biol Control, 2012, 23(5):417-420.
[9] Wang HY, Takasaki T, Fu SH, et al. Molecular epidemiological analysis of Japanese encephalitis virus in China[J]. J Gen Virol, 2007, 88(3):885-894. DOI:10.1099/vir.0.82185-0.
[10] 宋颂, 付士红, 李元元, 等. 贵州省2017年蚊虫及蚊传虫媒病毒调查研究[J]. 中国媒介生物学及控制杂志, 2018, 29(5):428-435, 461. DOI:10.11853/j.issn.1003.8280.2018.05.002.Song S, Fu SH, Li YY, et al. Investigation of mosquitoes and mosquito-borne viruses in Guizhou province, 2017[J]. Chin J Vector Biol Control, 2018, 29(5):428-435, 461. DOI:10.11853/j.issn.1003.8280.2018.05.002.
[11] 薛志静, 王君, 宋秀平, 等. 贵州省不同地区2018年蚊虫及蚊媒病毒调查[J]. 中国媒介生物学及控制杂志, 2019, 30(3):259-263. DOI:10.11853/j.issn.1003.8280.2019.03.007.Xue ZJ, Wang J, Song XP, et al. Investigation of mosquitoes and mosquito-borne viruses in different regions of Guizhou province, China, in 2018[J]. Chin J Vector Biol Control, 2019, 30(3):259-263. DOI:10.11853/j.issn.1003.8280.2019.03.007.
[12] 蒋维佳, 周敬祝, 唐光鹏, 等. 贵州省部分县市健康人群登革热感染情况调查[J]. 贵州医药, 2013, 37(2):164-165. DOI:10.3969/j.ISSN.1000-744X.2013.02.028.Jiang WJ, Zhou JZ, Tang GP, et al. Investigation on dengue fever infection in healthy population in some counties and cities of Guizhou province[J]. Guizhou Med J, 2013, 37(2):164-165. DOI:10.3969/j.ISSN.1000-744X.2013.02.028.
[13] Song S, Li YY, Fu SH, et al. Could Zika virus emerge in Mainland China? Virus isolation from nature in Culex quinquefasciatus, 2016[J]. Emerg Microbes Infect, 2017, 6(11):e93. DOI:10.1038/emi.2017.80.
[14] Fu SH, Song S, Liu H, et al. Zika virus isolated from mosquitoes:a field and laboratory investigation in China, 2016[J]. Sci China Life Sci, 2017, 60(12):1364-1371. DOI:10.1007/s11427-017-9196-8.
[15] 薛志静. 蚊媒病毒RT-hemi-nested PCR检测方法的建立及应用[D]. 泰安:山东第一医科大学, 2019.Xue ZJ. Establishment and application of the RT-hemi-nested PCR assay for mosquito-borne virus[D]. Tai'an:Shandong First Medical University, 2019.
[16] 卢剑云. 贵州省蚊虫名录[J]. 动物学杂志, 1959(2):74-76. DOI:10.13859/j.cjz.1959.02.012.Lu JY. Mosquito list in Guizhou province[J]. Chin J Zoology, 1959(2):74-76. DOI:10.13859/j.cjz.1959.02.012.
[17] 景晓, 李兆凰, 霍新北, 等. 骚扰阿蚊种群动态季节消长观察与分析[J]. 中国公共卫生, 2004, 20(8):936-937. DOI:10.3321/j.issn:1001-0580.2004.08.017.Jing X, Li ZH, Huo XB, et al. Observation and analysis on seasonal distribution of Armigeres subbalbeatus[J]. Chin J Public Health, 2004, 20(8):936-937. DOI:10.3321/j.issn:1001-0580.2004.08.017.
[18] 秦芝旭, 杜慧, 吴凡, 等. 伊蚊的监测与防治[J]. 中华卫生杀虫药械, 2017, 23(6):575-580. DOI:10.19821/j.1671-2781.2017.06.025.Qin ZX, Du H, Wu F, et al. Surveillance and control of Aedes[J]. Chin J Hyg Insect Equip, 2017, 23(6):575-580. DOI:10.19821/j.1671-2781.2017.06.025.
[19] 陈君, 刘奇男, 张平. 丹东市市区蚊虫的种类及其生态习性调查[J]. 中华卫生杀虫药械, 2013, 19(3):242-243. DOI:10.19821/j.1671-2781.2013.03.025.Chen J, Liu QN, Zhang P. Investigation on the species and ecological habits of mosquitoes in Dandong urban district[J]. Chin J Hyg Insect Equip, 2013, 19(3):242-243. DOI:10.19821/j.1671-2781.2013.03.025.
[20] 叶辉铭, 潘亮, 张忠英. 福建虫媒病毒研究概况[J]. 中国人兽共患病学报, 2007, 23(6):631-632. DOI:10.3969/j.issn.1002-2694.2007.06.031.Ye HM, Pan L, Zhang ZY. Study on arbovirus in Fujian province[J]. Chin J Zoonoses, 2007, 23(6):631-632. DOI:10.3969/j.issn.1002-2694.2007.06.031.
[21] Zhai YG, Wang HY, Sun XH, et al. Complete sequence characterization of isolates of Getah virus (genus Alphavirus, family Togaviridae) from China[J]. J Gen Virol, 2008, 89(Pt 6):1446-1453. DOI:10.1099/vir.0.83607-0.
[22] 邓掌, 张海林. 黄病毒及其相关疾病研究进展[J]. 国际病毒学杂志, 2009, 16(6):161-167. DOI:10.3760/cma.j.issn.1673-4092.2009.06.001.Deng Z, Zhang HL. Research progress of flavivirus and related diseases[J]. Internat J Virol, 2009, 16(6):161-167. DOI:10.3760/cma.j.issn.1673-4092.2009.06.001.
[23] Ye XF, Wang HY, Fu SH, et al. Etiological spectrum of clinically diagnosed Japanese encephalitis cases reported in Guizhou province, China, in 2006[J]. J Clin Microbiol, 2010, 48(4):1343-1349. DOI:10.1128/JCM.01009-09.
[24] Wang LH, Fu SH, Zhang HL, et al. Identification and isolation of Genotype-I Japanese encephalitis virus from encephalitis patients[J]. Virol J, 2010, 7:345. DOI:10.1186/1743-422X-7-345.
[25] Shu PY, Huang JH. Current advances in dengue diagnosis[J]. Clin Diagn Lab Immunol, 2004, 11(4):642-650. DOI:10.1128/CDLI.11.4.642-650.2004.
[26] 张硕, 李德新. 寨卡病毒和寨卡病毒病[J]. 病毒学报, 2016, 23(1):121-127. DOI:10.13242/j.cnki.bingduxuebao.002877.Zhang S, Li DX. Zika virus and Zika viral disease[J]. Chin J Virol, 2016, 23(1):121-127. DOI:10.13242/j.cnki.bingduxuebao.002877.

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