Objective To investigate and understand the fauna and distribution of fleas in Tibet autonomous region (Tibet), and to provide a basis for further research on the fauna of fleas in Tibet. Methods With Linzhi in southeastern Tibet as the spatial scope of investigation and research, small mammals and their parasitic fleas in farming areas at different altitudes were selected as the research objects. From October to November, 2018, large rat traps were used for the investigation and sampling of the small mammals and their parasitic fleas, and the samples were classified and identified in the laboratory. Results A batch of Palaeopsylla Wagner, 1903, specimens were collected and identified as P. helena Lewis, 1973, P. incurva Jordan, 1932, P. medimina Xie et Gong, 1989, and P. polyspina Xie et Gong, 1989, among which P. medimina and P. polyspina were found for the first time in Tibet, setting a new record for flea species in this region. This article recorded and reported the main identification characteristics, distribution areas, host animals, and the head and genital abdominal segments of the two newly recorded species. In addition, this article discussed the geographical distribution of Palaeopsylla in China. Conclusion Up to now, seven species of Palaeopsylla have been known in Tibet Autonomous Region, accounting for 23% of the total number of species in China.
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