浙江省宁波市登革热旧疫点白纹伊蚊越冬卵阳性率及登革病毒携带情况调查

马敏, 杨思嘉, 李保军, 马晓, 葛宝湘, 顾锋, 祝梦雨

doi:10.11853/j.issn.1003.8280.2021.01.012

中国媒介生物学及控制杂志 >

2021 , Vol. 32 >Issue 1: 60 - 64

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.01.012

媒介生物监测

浙江省宁波市登革热旧疫点白纹伊蚊越冬卵阳性率及登革病毒携带情况调查

展开

1. 宁波市疾病预防控制中心消毒与媒介生物防制所, 浙江宁波 315010;
2. 宁波市海曙区疾病预防控制中心, 浙江宁波 315012;
3. 宁海县疾病预防控制中心, 浙江宁海 315600;
4. 宁波市北仑区疾病预防控制中心, 浙江宁波 315800;
5. 慈溪市疾病预防控制中心, 浙江慈溪 315300

马敏,女,博士,助理研究员,主要从事媒介生物防制工作,E-mail:mamin2013@126.com

收稿日期: 2020-04-28

网络出版日期: 2021-02-20

基金资助

浙江省医药卫生科技计划项目（2019KY634）；宁波市医学科技计划项目（2017A29）；宁波市医疗卫生品牌学科现场流行病学项目（PPXK2018-10）；海曙区科技局社会发展类科研项目（201804F028）

收起

Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China

Expand

1. Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China;
2. Haishu District Center for Disease Control and Prevention, Ningbo, Zhejiang 315012, China;
3. Ninghai County Center for Disease Control and Prevention, Ninghai, Zhejiang 315600, China;
4. Beilun District Center for Disease Control and Prevention, Ningbo, Zhejiang 315800, China;
5. Cixi Center for Disease Control and Prevention, Cixi, Zhejiang 315300, China

Received date: 2020-04-28

Online published: 2021-02-20

Supported by

Supported by the Zhejiang Medicine Health Science and Technology Project (No. 2019KY634), Ningbo Medical Science Technology Project (No. 2017A29), Ningbo Health Branding Subject Fund Field Epidemiology (No. PPXK2018-10) and the Social Development Research Project of Haishu District Science and Technology Bureau (No. 201804F028)

Fold

摘要

目的了解宁波市登革热旧疫点白纹伊蚊越冬卵及其登革病毒携带情况，为登革热防控提供科学依据。方法 2018年12月至2019年3月在宁波市海曙区登革热旧疫点的核心区、警戒区和监控区，以及北仑区、慈溪市和宁海县等3个非疫点县（市、区）采集不同积水容器中的积水和积泥，在实验室条件下孵化白纹伊蚊卵，采用实时荧光定量反转录-聚合酶链式反应检测其登革病毒携带情况，并采用χ²检验和logistic回归模型分析白纹伊蚊越冬卵阳性率影响因素。结果共采集样本884份，白纹伊蚊越冬卵阳性率为11.99%。不同登革热防控区域、不同积水容器中白纹伊蚊越冬卵阳性率差异均有统计学意义（χ²=31.227，P<0.001；χ²=38.044，P<0.001）。多因素分析结果显示疫点警戒区、监控区和非疫点白纹伊蚊越冬卵阳性率分别是疫点核心区的5.33（95%CI：1.16～24.49）、6.26（95%CI：1.37～28.65）和11.56（95%CI：2.75～48.50）倍。废旧轮胎和植被性容器中白纹伊蚊越冬卵阳性率分别是固定性容器的3.59（95%CI：1.79～7.20）和3.77（95%CI：1.82～7.79）倍。登革病毒核酸检测结果均为阴性。结论登革热旧疫点警戒区、监控区和非疫点及废旧轮胎和植被性容器属当地白纹伊蚊越冬卵的主要孳生场所或孳生地，建议相关部门在早春及时采取以清除积水为主的防蚊灭蚊工作。

关键词： 白纹伊蚊; 越冬卵; 影响因素; 登革病毒; 实时荧光定量反转录-聚合酶链式反应

本文引用格式

马敏, 杨思嘉, 李保军, 马晓, 葛宝湘, 顾锋, 祝梦雨 . 浙江省宁波市登革热旧疫点白纹伊蚊越冬卵阳性率及登革病毒携带情况调查[J]. 中国媒介生物学及控制杂志, 2021 , 32(1) : 60 -64 . DOI: 10.11853/j.issn.1003.8280.2021.01.012

Abstract

Objective To investigate overwintering Aedes albopictus eggs and the dengue virus carried by the eggs in the former dengue epidemic focus in Ningbo, Zhejiang province, China, and to provide scientific evidence for dengue control. Methods During December 2018 to March 2019, standing water and sludge in containers were collected from the former dengue epidemic focus (including the core area, warning area, and monitoring area) of Haishu district and three non-epidemic areas (Beilun district, Cixi city, and Ninghai county) in Ningbo, for incubation of Ae. albopictus eggs under laboratory conditions. Real-time RT-PCR was used to detect the dengue virus carried by the eggs. The chi-square test and logistic regression analysis were used to determine the influencing factors of the positive rate of overwintering Ae. albopictus eggs. Results A total of 884 samples were collected, and the positive rate of overwintering Ae. albopictus eggs was 11.99%. There were significant differences in the positive rate of overwintering Ae. albopictus eggs between different dengue control areas (χ²=31.227, P<0.001) and between different water containers (χ²=38.044, P<0.001). According to multivariate analysis, the positive rates for the warning area, monitoring area, and non-epidemic area were 5.33 (95% confidence interval[CI]:1.16-24.49), 6.26 (95%CI:1.37-28.65), and 11.56 (95%CI:2.75-48.50) times that for the core area, respectively. The positive rates for waste tires and vegetative containers were 3.59 (95%CI:1.79-7.20) and 3.77 (95%CI:1.82-7.79) times that for stationary containers, respectively. All test results for the nucleic acid of dengue virus were negative. Conclusion The warning area and monitoring area of the former dengue epidemic focus, non-epidemic areas, waste tires, and vegetative containers are the main breeding sites for overwintering Ae. albopictus eggs. It is suggested that relevant departments should take timely measures focusing on the removal of standing water to control mosquitoes in early spring.

Key words： Aedes albopictus; Overwintering egg; Influencing factor; Dengue virus; Real-time RT-PCR

参考文献

[1] 郭颂,凌锋,王金娜,等. 浙江省不同地理株白纹伊蚊mtDNA-CO I基因多态性研究[J]. 中国人兽共患病学报,2016,32(2):133-136,147. DOI:10.3969/j.issn.1002-2694.2016.02.007.Guo S,Ling F,Wang JN,et al. Genetic polymorphism analysis of cytochrome c oxidase subunit I gene in Aedes albopictus from Zhejiang province,China[J]. Chin J Zoonoses,2016,32(2):133-136,147. DOI:10.3969/j.issn.1002-2694.2016.02.007.
[2] Guo X,Zhao T,Dong Y,et al. Survival and replication of Dengue-2 virus in diapausing eggs of Aedes albopictus (Diptera:Culicidae)[J]. J Med Entomol,2007,44(3):492-497. DOI:10.1093/jmedent/44.3.492.
[3] 马敏,马晓,杨思嘉,等. 登革热病毒的垂直传递和伊蚊滞育研究进展[J]. 中国媒介生物学及控制杂志,2018,29(5):539-542. DOI:10.11853/j.issn.1003.8280.2018.05.031.Ma M,Ma X,Yang SJ,et al. Progress in studies on vertical transmission of dengue virus and egg diapause in Aedes mosquitoes[J]. Chin J Vector Biol Control,2018,29(5):539-542. DOI:10.11853/j.issn.1003.8280.2018.05.031.
[4] Yi B,Chen Y,Ma X,et al. Incidence dynamics and investigation of key interventions in a dengue outbreak in Ningbo city,China[J]. PLoS Negl Trop Dis,2019,13(8):e0007659. DOI:10.1371/journal.pntd.0007659.
[5] 马敏,徐明,易波,等. 登革热暴发后媒介伊蚊的应急监测[J]. 中国媒介生物学及控制杂志,2019,30(4):374-378. DOI:10.11853/j.issn.1003.8280.2018.04.004.Ma M,Xu M,Yi B,et al. Emergency surveillance of vector Aedes after a dengue outbreak[J]. Chin J Vector Biol Control,2019,30(4):374-378. DOI:10.11853/j.issn.1003.8280.2018.04.004.
[6] 中国疾病预防控制中心.登革热防治技术指南[EB/OL]. (2014-09-29)[2020-10-23]. http://www.chinacdc.cn/jkzt/crb/zl/dgr/jszl_2235/201409/t20140929_104958.html.Chinese Center for Disease Control and Prevention. Technical guidelines for dengue fever control[EB/OL]. (2014-09-29)[2020-10-23]. http://www.chinacdc.cn/jkzt/crb/zl/dgr/jszl_2235/201409/t20140929_104958.html.
[7] 陆宝麟,吴厚永. 中国重要医学昆虫分类与鉴别[M]. 郑州:河南科学技术出版社,2003:26-35.Lu BL,Wu HY. Classification and identification of important medical insects of China[M]. Zhengzhou:Henan Science and Technology Publishing House,2003:26-35.
[8] 杨思嘉,马晓,朱光锋,等. 宁波市2016年白纹伊蚊越冬孳生地调查及影响因素分析[J]. 中国媒介生物学及控制杂志,2017,28(1):69-71. DOI:10.11853/j.issn.1003.8280.2017. 01.019.Yang SJ,Ma X,Zhu GF,et al. Analysis of the breeding sources of overwintering Aedes albopictus and influencing factors in Ningbo city,2016[J]. Chin J Vector Biol Control,2017,28(1):69-71. DOI:10.11853/j.issn.1003.8280.2017.01.019.
[9] 马敏,杨思嘉,马晓,等. 宁波市白纹伊蚊越冬卵调查[J]. 预防医学,2019,31(4):397-399,404. DOI:10.19485/j.cnki.issn 2096-5087.2019.04.018.Ma M,Yang SJ,Ma X,et al. Investigation on the overwintering eggs of Aedes albopictus in Ningbo city[J]. J Prev Med,2019,31(4):397-399,404. DOI:10.19485/j.cnki.issn2096-5087.2019. 04.018.
[10] 韦凌娅,孔庆鑫,王慧敏. 双层叠帐法与BG-trap诱捕器法在登革热应急蚊媒监测中的捕蚊效果比较[J]. 中国媒介生物学及控制杂志,2019,30(1):65-68. DOI:10.11853/j.issn.1003. 8280.2019.01.014.Wei LY,Kong QX,Wang HM. Effectiveness of double mosquito net and BG-trap for emergency vector surveillance during dengue fever epidemics:a comparative study[J]. Chin J Vector Biol Control,2019,30(1):65-68. DOI:10.11853/j.issn.1003.8280. 2019.01.014.
[11] 曹一鸥,刘学成,祝小平,等. 2008-2009年四川省登革热蚊媒监测结果分析[J]. 中国媒介生物学及控制杂志,2011,22(2):117-120.Cao YO,Liu XC,Zhu XP,et al. Analysis on the surveillance results of mosquito vectors of dengue fever from 2008 to 2009 in Sichuan province[J]. Chin J Vector Biol Control,2011,22(2):117-120.
[12] 于虹,赵彤言,陆宝麟. 北京市轮胎积水滋生蚊虫的调查[J]. 中国媒介生物学及控制杂志,2000,11(2):107-109. DOI:10.3969/j.issn.1003-4692.2000.02.007.Yu H,Zhao TY,Lu BL. Survey of breeding of mosquitoes in used tires in Beijing[J]. Chin J Vector Biol Control,2000,11(2):107-109. DOI:10.3969/j.issn.1003-4692.2000.02.007.
[13] 徐友祥,王韶华. 室外废旧轮胎白纹伊蚊的越冬情况调查[J]. 中国媒介生物学及控制杂志,2006,17(3):175-176. DOI:10.3969/j.issn.1003-4692.2006.03.005.Xu YX,Wang SH. The hibernating investigation of Aedes albopictus in abandon and shabby tyre outdoor[J]. Chin J Vector Biol Control,2006,17(3):175-176. DOI:10.3969/j.issn.1003-4692.2006.03.005.
[14] 李美青,刘永权,黄燕惠,等. 广州市城乡不同季节白纹伊蚊密度、带病毒情况及抗药性监测[J]. 中国媒介生物学及控制杂志,2013,24(2):108-111.Li MQ,Liu YQ,Huang YH,et al. Seasonal variation in population density,dengue virus carriage,and dichlorvos resistance of Aedes albopictus in urban and rural areas of Guangzhou city,China[J]. Chin J Vector Biol Control,2013,24(2):108-111.
[15] 吴彤宇,秦娜,张静,等. 2009-2015年天津市登革热流行风险分析[J]. 现代预防医学,2016,43(11):1925-1927,1943.Wu TY,Qin N,Zhang J,et al. Dengue fever epidemic risk in Tianjin[J]. Mod Prev Med,2016,43(11):1925-1927,1943.
[16] 刘晓娜,吴能简,吴崧霖,等. 深圳市坪山新区2016年登革热媒介监测结果分析[J]. 中国媒介生物学及控制杂志,2017,28(3):283-285. DOI:10.11853/j.issn.1003.8280.2017.03.023.Liu XN,Wu NJ,Wu SL,et al. Analysis of surveillance for dengue vector in Pingshan district of Shenzhen city,2016[J]. Chin J Vector Biol Control,2017,28(3):283-285. DOI:10.11853/j.issn.1003.8280.2017.03.023.
[17] 李成玲,江毅民,胡志刚,等. 广州地区白纹伊蚊体内登革病毒的检测[J]. 热带医学杂志,2008,8(11):1128-1129. DOI:10.3969/j.issn.1672-3619.2008.11.007.Li CL,Jiang YM,Hu ZG,et al. Detection of dengue virus in Aedes albopictus in Guangzhou[J]. J Trop Med,2008,8(11):1128-1129. DOI:10.3969/j.issn.1672-3619.2008.11.007.
[18] 舒莉萍,左丽,郝牧,等. 贵州自然界白纹伊蚊体内登革病毒带毒情况[J]. 中国公共卫生,2004,20(5):532-534. DOI:10.3321/j.issn:1001-0580.2004.05.011.Shu LP,Zuo L,Hao M,et al. Investigation on dengue virus from Aedes albopictus field in Guizhou[J]. Chin J Public Health,2004,20(5):532-534. DOI:10.3321/j.issn:1001-0580.2004.05.011.
[19] 张海林,自登云,米竹青,等. 云南白纹伊蚊分布特点及与虫媒病毒的关系[J]. 中国媒介生物学及控制杂志,2001,12(2):103-105.Zhang HL,Zi DY,Mi ZQ,et al. Characterized distribution of Aedes albopictus and their relation with Arbovirus in Yunnan province[J]. Chin J Vector Biol Control,2001,12(2):103-105. DOI:10.3969/j.issn.1003-4692.2001.02.007.
[20] 阚乃鹏,翁育伟,林琦,等. 福建省白纹伊蚊中登革病毒的分离与鉴定[J]. 病毒学报,2019,35(1):71-76. DOI:10.13242/j.cnki.bingduxuebao.003497.Kan NP,Weng YW,Lin Q,et al. Isolation and identification of a dengue virus strain from Aedes albopictus in Fujian province,China[J]. Chin J Virol,2019,35(1):71-76. DOI:10.13242/j.cnki.bingduxuebao.003497.
[21] 郭晓霞. 白纹伊蚊卵滞育与登革Ⅱ型病毒传播关系的研究[D]. 重庆:西南农业大学,2003.Guo XX. The relationship between diapausing eggs of infected Aedes albopictus and transmission of dengue 2 virus[D]. Chongqing:Southwest Agricultural University,2003.
[22] 宋秀玲,黄炯烈,郑小英,等. 成蚊传播登革病毒媒介效能的研究进展[J]. 热带医学杂志,2005,5(2):251-254. DOI:10.3969/j.issn.1672-3619.2005.02.049.Song XL,Huang JL,Zheng XY,et al. Progress of the research on the efficacy of the adult mosquito as vector for transmitting dengue fever[J]. J Trop Med,2005,5(2):251-254. DOI:10. 3969/j.issn.1672-3619.2005.02.049.
[23] 王金娜,侯娟,刘钦梅,等. 白纹伊蚊登革病毒检测研究进展[J]. 中国媒介生物学及控制杂志,2020,31(3):376-379. DOI:10.11853/j.issn.1003.8280.2020.03.028.Wang JN,Hou J,Liu QM,et al. Research progress in dengue virus detection in Aedes albopictus[J]. Chin J Vector Biol Control,2020,31(3):376-379. DOI:10.11853/j.issn.1003. 8280.2020.03.028.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献