目的 比较研究吡丙醚水乳剂、S-烯虫酯颗粒剂、S-烯虫酯微胶囊悬浮剂3种昆虫生长调节剂对楼宇地下车库集水井致倦库蚊幼蚊羽化抑制效果，为幼蚊控制提供科学选用依据。方法 2019年在广州市某小区楼宇地下车库集水井投放吡丙醚水乳剂、S-烯虫酯颗粒剂和S-烯虫酯微胶囊悬浮剂控制致倦库蚊，用勺舀法在投药后第3、7、14、21和28天分别采集集水井中幼蚊、蛹带回实验室观察羽化情况。结果 投药后第3天经吡丙醚水乳剂、S-烯虫酯颗粒剂和S-烯虫酯微胶囊悬浮剂处理集水井中致倦库蚊幼蚊羽化率均明显低于对照集水井中致倦库蚊幼蚊羽化率（吡丙醚水乳剂：χ2=176.414，P<0.001；S-烯虫酯颗粒剂：χ2=13.268，P<0.001；S-烯虫酯微胶囊悬浮剂：χ2=34.784，P<0.001），吡丙醚水乳剂处理的集水井中致倦库蚊幼蚊羽化率从投药后第3~28天均明显低于对照组（P<0.001）；S-烯虫酯颗粒剂处理的集水井中致倦库蚊幼蚊羽化率从投药后第3~21天均明显低于对照组（P<0.001）；S-烯虫酯微胶囊悬浮剂处理的集水井中致倦库蚊幼蚊羽化率从投药后第3~14天均明显低于对照组（P<0.001）。结论 吡丙醚水乳剂、S-烯虫酯颗粒剂、S-烯虫酯微胶囊悬浮剂均能很好地降低楼宇集水井中致倦库蚊幼蚊的羽化率。其中吡丙醚水乳剂因半衰期相对较长，控制效果持续时间较长；而烯虫酯半衰期短，控制效果持续时间较短，但适合在环保要求较高场所使用。
刘杰, 李晓宁, 江毅民, 梁雪莹, 陈宗遒, 贺征, 甘露, 胡来贵, 罗雷
. 吡丙醚、S–烯虫酯对楼宇地下车库集水井致倦库蚊幼蚊羽化抑制效果比较研究[J]. 中国媒介生物学及控制杂志, 2020
, 31(6)
: 702
-705
.
DOI: 10.11853/j.issn.1003.8280.2020.06.016
Objective To investigate the inhibitory effect of pyriproxyfen water emulsion, S-methoprene granules, and S-methoprene microcapsule suspension on the emergence of Culex pipiens quinquefasciatus larvae in water collecting wells of underground garages in buildings, and to provide a scientific basis for the control of mosquito larvae. Methods In 2019, pyriproxyfen water emulsion, S-methoprene granules, and S-methoprene microcapsule suspension were placed in the water collecting wells of underground garages in buildings of a residential area in Guangzhou, China to control Cx. pipiens quinquefasciatus. The spoon method was used to collect the larvae and pupae of Cx. pipiens quinquefasciatus in the water collecting wells on days 3, 7, 14, 21, and 28 after the application of pyriproxyfen water emulsion, S-methoprene granules, and S-methoprene microcapsule suspension, and the larvae and pupae were taken back to the laboratory to observe emergence. Results On day 3 after application, the water collecting wells treated by pyriproxyfen water emulsion, S-methoprene granules, and S-methoprene microcapsule suspension had a significantly lower emergence rate of Cx. pipiens quinquefasciatus larvae than the control water collecting wells (pyriproxyfen water emulsion:χ2=176.414, P<0.001; S-methoprene granules:χ2=13.268, P<0.001; S-methoprene microcapsule suspension:χ2=34.784, P<0.001). On days 3-28 after application, the water collecting wells treated by pyriproxyfen water emulsion had a significantly lower emergence rate of Cx. pipiens quinquefasciatus larvae than the control water collecting wells (P<0.001). On days 3-21 after application, the water collecting wells treated by S-methoprene granules had a significantly lower emergence rate of Cx. pipiens quinquefasciatus larvae than the control water collecting wells (P<0.001). On days 3-14 after application, the water collecting wells treated by S-methoprene microcapsule suspension had a significantly lower emergence rate of Cx. pipiens quinquefasciatus larvae than the control water collecting wells (P<0.001). Conclusion Pyriproxyfen water emulsion, S-methoprene granules, and S-methoprene microcapsule suspension can reduce the emergence rate of Cx. pipiens quinquefasciatus larvae in water collecting wells of buildings, among which pyriproxyfen water emulsion has relatively long half-life and control effect duration, while S-methoprene has short half-life and control effect duration, but it is suitable for use in places with high environmental protection requirements.
[1] Ishaaya I,Horowitz AR. Novel phenoxy juvenile hormone analog (Pyriproxyfen) suppresses embryogenesis and adult emergence of Sweetpotato whitefly (Homoptera:Aleyrodidae)[J]. J Econ Entomol,1992,85(6):2113-2117. DOI:10.1093/jee/85.6.2113.
[2] Aribi N,Smagghe G,Lakbar S,et al. Effects of pyriproxyfen,a juvenile hormone analog,on development of the mealworm,Tenebriomolitor[J]. Pestic Biochem Phys,2006,84(1):55-62. DOI:10.1016/j.pestbp.2005.05.008.
[3] Sullivan J. Environmental fate of pyriproxyfen[R]. Sacramento,CA:Department of Pesticide Regulation,2000:1-9.
[4] 徐仁权,刘洪霞,冷培恩,等. 0.5%吡丙醚颗粒剂对白纹伊蚊控制效果的研究[J]. 中国媒介生物学及控制杂志,2010,21(4):297-299. Xu RQ,Liu HX,Leng PE,et al. Effectiveness of 0.5% pyriproxyfen granules for Aedes albopictus control[J]. Chin J Vector Biol Control,2010,21(4):297-299.
[5] 李广,冉会来,张俊玲,等. 4种杀虫剂对淡色库蚊幼虫的药效对比试验[J]. 中国媒介生物学及控制杂志,2012,23(1):63-65. Li G,Ran HL,Zhang JL,et al. Comparative experiment study of the efficacy of four kinds of insecticides against mosquito larvae[J]. Chin J Vector Biol Control,2012,23(1):63-65.
[6] 苏天运. 美国烯虫酯研究开发和应用:50年成功回顾[J]. 中华卫生杀虫药械,2018,24(4):317-325. DOI:10.19821/j.1671-2781.2018.04.001. Su TY. Research,development and application of S-methoprene in the USA:50 years of excellence[J]. Chin J Hyg Insectic Equip,2018,24(4):317-325. DOI:10.19821/j.1671-2781.2018.04.001.
[7] 孙海燕,陈伟国,董瑞华,等. 10%吡丙醚EC对家蚕的毒性测试[J]. 蚕桑通报,2008,39(2):18-20. DOI:10.3969/j.issn.0258-4069.2008.02.005. Sun HY,Chen WG,Dong RH,et al. The toxicity test of the 10% EC pyriproxyfen on silkworm[J]. Bull Sericulture,2008,39(2):18-20. DOI:10.3969/j.issn.0258-4069.2008.02.005.
[8] 刘洪霞,冷培恩,徐劲秋,等. 淡色库蚊对吡丙醚的抗性选育及抗性风险评估[J]. 上海预防医学,2015,27(9):533-536. DOI:10.19428/j.cnki.sjpm.2015.09.004. Liu HX,Leng PE,Xu JQ,et al. Selection and risk assessment of resistance to pyriproxyfen in Culex pipiens pallens[J]. Shanghai J Prev Med,2015,27(9):533-536. DOI:10.19428/j.cnki.sjpm.2015.09.004.
[9] 苏天运. 生物理念杀蚊幼剂的历史与现状及未来[J]. 中华卫生杀虫药械,2014,20(1):1-5,9. DOI:10.19821/j.1671-2781.2014.01.002. Su TY. History,present and the future of biorational larvicides against mosquitoes[J]. Chin J Hyg Insectic Equip,2014,20(1):1-5,9. DOI:10.19821/j.1671-2781.2014.01.002.
[10] 徐友祥,张书志,王韶华,等. S-烯虫酯控制城市雨水井蚊幼虫羽化的效果观察[J]. 中华卫生杀虫药械,2017,23(1):22-25. DOI:10.19821/j.1671-2781.2017.01.005. Xu YX,Zhang SZ,Wang SH,et al. The inhibition effect of S-methoprene sustained-release block on the eclosion of mosquito larvae in urban rainwater wells[J]. Chin J Hyg Insectic Equip,2017,23(1):22-25. DOI:10.19821/j.1671-2781.2017.01.005.
[11] United States Environmental Protection Agency. FactS-Methoprene[EB/OL]. (2001-06)[2020-03]. https://archive.epa.gov/pesticides/reregistration/web/pdf/0030fact.pdf.
[12] Amin AM,White GB. Resistance potential of Culex quinquefasciatus against the insect growth regulators methoprene and diflubenzuron[J]. Ent Exp Appl,1984,36(1):69-76. DOI:10.1111/j.1570-7458.1984.tb03409.x.
[13] Su TY,Cheng ML. Cross resistances in spinosad-resistant Culex quinquefasciatus (Diptera:Culicidae)[J]. J Med Entomol,2014,51(2):428-435. DOI:10.1603/ME13207.
[14] Su TY,Thieme J,Ocegueda C,et al. Resistance to Lysinibacillus sphaericus and other commonly used pesticides in Culex pipiens (Diptera:Culicidae) from Chico,California[J]. J Med Entomol,2018,55(2):423-428. DOI:10.1093/jme/tjx235.
