目的 对2019年衢州市本地登革热暴发疫情中检出的登革病毒1型进行序列测定和分子特征分析。方法 采集患者血清标本，采用胶体金免疫层析法和实时荧光定量PCR（RT-qPCR）方法分别检测登革病毒NS1抗原和病毒核酸；采用RT-qPCR方法扩增登革病毒E基因后进行序列测定，并与不同国家和地区的登革病毒株进行同源性和进化树分析。结果 从患者血清标本中检测到登革病毒NS1抗原和病毒核酸，经基因序列比对及进化分析，8株登革病毒均为登革1型的基因Ⅰ型，与JF960228（2010年新加坡分离株）互为姐妹群，且毒力位点1处发生变异。结论 从病原学、血清学和分子生物学证实该起暴发疫情是由登革1型病毒引起，该毒株有可能来源于东南亚，应加强中国与该地区登革热跨境传播的防控。
Objective To determine the nucleic acid sequence of the dengue type 1 virus (DENV-1) detected in the local dengue outbreak in Quzhou, Zhejiang province, China, 2019, and to analyze its molecular characteristics. Methods Serum samples were collected from dengue patients; colloidal gold immunochromatographic assay and quantitative real-time PCR (RT-qPCR) were used to detect the NS1 antigen and nucleic acid of dengue virus, respectively. The RT-qPCR assay was used to amplify the envelope gene of dengue virus, which was then sequenced. The genotype of the isolated strains was analyzed, and the homology and phylogenetic analyses were performed with the dengue strains isolated from other countries and regions. Results The serum samples of patients were positive for the NS1 antigen and nucleic acid of dengue virus. The gene sequence alignment and phylogenetic analysis showed that 8 dengue virus strains all belonged to DENV-1 subgenotype GI, which had the closest phylogenetic relationship with the strain JF960228 (Singapore, 2010), with a mutation at virulence locus 1. Conclusion It was confirmed by etiology, serology, and molecular biology that the local dengue outbreak was caused by DENV-1, which may originate from Southeast Asia. It is necessary to strengthen the prevention and control of cross-border spread of dengue fever in China and this region.
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