纳米孔测序技术研究进展

闫冬明, 赵宁, 赵春春, 王君, 宋秀平, 朱彩英, 刘起勇, 张钦凤

doi:10.11853/j.issn.1003.8280.2020.03.029

中国媒介生物学及控制杂志 >

2020 , Vol. 31 >Issue 3: 380 - 384

DOI: https://doi.org/10.11853/j.issn.1003.8280.2020.03.029

综述

纳米孔测序技术研究进展

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1 山东第一医科大学公共卫生学院, 山东泰安 271016;
2 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206

闫冬明,男,在读硕士,主要从事病媒生物与虫媒病研究工作,Email:yandongmingcool@163.com

收稿日期: 2020-03-04

网络出版日期: 2020-06-20

基金资助

国家重点研发计划（2016YFC1200802）

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Research progress in MinION sequencing technology and its application

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1 School of Public Health of Shandong First Medical University, Tai'an 271016, Shandong Province, China;
2 State Key Laboratory of Infectious Disease Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received date: 2020-03-04

Online published: 2020-06-20

Supported by

Supported by the National Key R&D Program of China (No. 2016YFC1200802)

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摘要

牛津纳米孔技术公司（ONT）的纳米孔测序仪（MinION）于2014年发布，代表了1种新型测序技术的诞生。该设备简单便携，可通过1台笔记本电脑在现场完成目标检测，它的特点是单分子测序、测序数据实时监控、超长读长和低成本等，目前已被应用于微生物种群鉴定、全基因组测序、新物种发现等方面。尽管该技术近几年取得了令人可喜的成绩，但同时也出现了一些问题和挑战，该文综述了MinION的技术特征及开发应用，并探讨了其发展前景。

关键词： 纳米孔测序仪; 单分子测序; 应用

本文引用格式

闫冬明, 赵宁, 赵春春, 王君, 宋秀平, 朱彩英, 刘起勇, 张钦凤 . 纳米孔测序技术研究进展[J]. 中国媒介生物学及控制杂志, 2020 , 31(3) : 380 -384 . DOI: 10.11853/j.issn.1003.8280.2020.03.029

Abstract

In 2014, the nanopore sequencer MinION was released by Oxford Nanopore Technologies (ONT), representing the birth of a novel sequencing technology. The device is extremely portable and can carry out target detection in the field through a laptop. It is characterized by single-molecule sequencing, real-time monitoring of sequencing data, ultra-long read lengths, and low cost. It has been applied to the identification of microbial populations, whole genome sequencing, discovery of new species, etc. Although the technology has made impressive achievements in recent years, there are also some problems and challenges. This review mainly summarizes the technical characteristics and development fields of the MinION sequencer, and discusses its development prospects.

Key words： Nanopore sequencer MinION; Single-molecule sequencing; Application

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