目的 嗅觉系统在蚊虫吸血和寻找产卵地等活动中发挥着十分重要的作用。该研究对白纹伊蚊吸血前后气味受体（odorant receptors，ORs）OR10基因的表达水平进行探讨。方法 通过反转录-聚合酶链式反应（RT-PCR）方法扩增白纹伊蚊OR10，采用荧光定量PCR检测雄蚊、吸血雌蚊和未吸血雌蚊OR10基因的表达量。结果 扩增获得OR10基因的开放阅读框全长为1 128 bp，编码376个氨基酸。与埃及伊蚊和冈比亚按蚊的OR10基因的氨基酸序列一致性分别为86.13%和69.69%。荧光定量PCR发现雄蚊OR10基因的表达量最低为1，雌蚊OR10基因的表达量为1.535±0.274，高于雄蚊，吸血后的雌蚊OR10基因的表达量最高，为2.284±0.203，且未吸血与吸血后的雌蚊OR10基因的表达量差异有统计学意义（F=32.067，P=0.001）。结论 白纹伊蚊吸血前后气味受体OR10基因表达水平差异有统计学意义，进一步辅证了OR10蛋白可能参与蚊虫吸血后寻找产卵地的行为。
Objective The olfactory system plays an important role in mosquito behaviors such as blood sucking and oviposition site selection. This study compared the expression level of odorant receptor 10 gene (OR10) in Aedes albopictus before and after blood sucking. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was performed to amplify the OR10 gene, and quantitative real-time PCR was used to determine the expression levels of OR10 gene in male Ae. albopictus, and female Ae. albopictus before and after blood sucking. Results The open reading frame of the OR10 gene in Ae. albopictus obtained by amplification was 1 128 bp in length, encoding 376 amino acids. The amino acid sequence had an identity of 86.13% and 69.69%, respectively, with that in Ae. aegypti and Anopheles gambiae. The results of quantitative real-time PCR showed that male Ae. albopictus had the lowest expression level of OR10 gene of 1; female Ae. albopictus had a higher expression level (1.535±0.274) than male Ae. albopictus; female Ae. albopictus had the highest expression level of OR10 gene (2.284±0.203) after blood sucking; the expression level of OR10 gene in female Ae. albopictus changed significantly before and after blood sucking (F=32.067, P=0.001). Conclusion The expression level of OR10 in Ae. albopictus changes significantly before and after blood sucking, which further proves that OR10 may be involved in the mosquito behavior of oviposition site selection after blood sucking.
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