Objective To analyze Plasmodium vivax merozoite surface protein 1 (PvMSP-1) in imported cases and local cases in Liaoning province, China in the past ten years, and to perform a genotype comparison of PvMSP-1 (using BLAST) and homology analysis of the phylogenetic tree. Methods Plasmodium vivax samples were collected from cases during 2008-2018. The genomic DNAs of P. vivax were extracted and subjected to nested polymerase chain reaction-based amplification, sequencing, sequence alignment, and homology analysis using the primers designed based on the ICB5-ICB6 fragment. Results A total of 31 nucleic acid samples (13 Sal-1[located around position 470 bp] samples and 18 Belem[located around position 400 bp] samples) of P. vivax were analyzed. The resulting target products were sent to Shanghai Omicsspace Biotech Co., Ltd. for sequencing. The phylogenetic tree comparison analysis revealed that the 31 samples were comprised of 13 Sal-1 samples, 5 Belem samples, and 13 type Ⅲ samples. The ICB5-ICB6 fragment of the PvMSP-1 gene was highly polymorphic, and the source of infection could be determined by combining the DNA sequences and the epidemiological investigation. Conclusion The genotypes of PvMSP-1 in imported and local cases in Liaoning province in the past ten years are types Sal-1,Ⅲ, and Belem, with the former two as the dominant types.
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