目的 解决玻片固定液干燥过快问题,改良固定液配方。方法 为减缓固定液干燥速度,将5 ml甘油加入封片剂,保持湿度;并将原配方50%质量分数葡萄糖溶液5 ml改为3 ml,降低粘度。结果 新的封片剂配方为阿拉伯胶12 g,水合氯醛晶体20 g,冰醋酸5 ml,甘油5 ml,50%质量分数葡萄糖溶液3 ml,蒸馏水30 ml。结论 该封片剂干燥速度适中,适用于我国北方小型昆虫标本制备。
Objective To improve the formula of slide mounting medium, and to solve the problem that the slide mounting medium dries too fast. Methods In order to reduce the drying rate of the slide mounting medium, 5 ml glycerol was added to the medium to maintain the humidity; glucose was reduced from 5 ml (old formula) to 3 ml to reduce the viscosity. Results The new medium formula was as follows:acacia 12 g, chloral hydrate crystal 20 g, glacial acetic acid 5 ml, glycerol 5 ml, 50%w/w glucose syrup 3 ml, and distilled water 30 ml. Conclusion The drying rate of this slide mounting medium is in the moderate range, which is suitable for the preparation of small insect specimens in northern China.
[1] Hira PR,Assad RM,Okasha G,et al. Myiasis in Kuwait:nosocomial infections caused by Lucilia sericata and Megaselia scalaris[J]. Am J Trop Med Hyg,2004,70(4):386-389. DOI:10.4269/ajtmh.2004.70.386.
[2] Reibe S,Madea B. Use of Megaselia scalaris (Diptera:Phoridae) for post-mortem interval estimation indoors[J]. Parasitol Res,2010,106(3):637-640. DOI:10.1007/s00436-009-1713-5.
[3] Disney RHL. Scuttle flies Diptera,Phoridae (except Megaselia)[J]. Handb Identif Br Insects,1983,10:1-81.
[4] Disney RHL. Scuttle flies:the phoridae[M]. London:Chapman and Hall,1994,xii+467.
[5] Upton MS. Aqueous gum-chloral slide mounting media:an historical review[J]. Bull Entomol Res,1993,83(2):267-274. DOI:10.1017/S0007485300034763.
[6] Nakayama H. Systematic and morphological evaluations of a new species of Kuenburgia schmitz (Diptera:Phoridae) with remarkable asymmetry of the male genitalia,including a bifurcated left surstylus[J]. Entomol Sci,2008,11(2):179-188. DOI:10.1111/j.1479-8298.2008.00269.x.
[7] Disney RHL. The preservation of small diptera[J]. Entomol Mon Mag,2001,137:155-159.