目的 了解云南省登革热重点地区埃及伊蚊对11种杀虫剂的抗药性水平。方法 2017年8-9月分别从景洪市城区和耿马傣族佤族自治县（耿马县）孟定镇采集埃及伊蚊蛹或幼虫，在实验室繁殖至子一代（F1），采用成蚊滤纸接触筒法，测定其对11种杀虫剂的抗药性。结果 景洪市城区埃及伊蚊对氯菊酯、氯氟氰菊酯、高效氯氰菊酯、溴氰菊酯、顺式氯氰菊酯、马拉硫磷、杀螟硫磷、恶虫威、残杀威、氟虫腈和滴滴涕（DDT）的24 h后校正死亡率分别为0.92%、0、3.19%、91.40%、20.43%、85.86%、100%、97.71%、96.67%、99.41%和1.96%，KT50分别为>60、>60、53.83、45.39、48.25、>60、>60、36.34、32.13、>60 min和KT50无法计算；耿马县孟定镇埃及伊蚊对上述常用杀虫剂的24 h后校正死亡率分别为1.43%、3.01%、11.35%、60.27%、5.96%、100%、100%、100%、95.68%、95.95%和0.74%，KT50分别为>60、>60、26.48、16.35、22.67、>60、>60、35.03、40.17、>60 min和KT50无法计算。结论 景洪市和孟定镇埃及伊蚊对拟除虫菊酯类和有机氯类（DDT）杀虫剂均产生了不同程度的抗药性，对有机磷类、氨基甲酸酯类和苯基吡唑类（氟虫腈）杀虫剂仍然敏感。应因地制宜，停用或者限制使用已经产生抗性的杀虫剂。
Objective To investigate the resistance level of Aedes aegypti to 11 insecticides in the key areas of dengue fever in Yunnan province, China. Methods From August 2017 to September 2017, the pupae or larvae of Ae. aegypti were collected in Jinghong city and Mengding town, Gengma Dai and Wa Autonomous County, and then they were bred to the first generation (F1) in the laboratory. The resistance of the first generation to 11 insecticides was determined by the filter paper contact method. Results The 24 h corrected mortality rates of adult Ae. aegypti in Jinghong city to permethrin, λ-cyhalothrin, beta-cypermethrin, deltamethrin, α-cypermethrin, malathion, fenitrothion, bendiocarb, propoxur, fipronil, and DDT were 0.92%, 0, 3.19%, 91.40%, 20.43%, 85.86%, 100%, 97.71%, 96.67%, 99.41%, and 1.96%, respectively, and KT50 was >60, >60, 53.83, 45.39, 48.25, >60, >60, 36.34, 32.13, >60 min, and not calculable, respectively. The 24 h mortality rates of adult Ae. aegypti in Mengding town to the above mentioned insecticides were 1.43%, 3.01%, 11.35%, 60.27%, 5.96%, 100%, 100%, 100%, 95.68%, 95.95%, and 0.74%, respectively, and the corresponding KT50 was >60, >60, 26.48, 16.35, 22.67, >60, >60, 35.03, 40.17, >60 min, and not calculable, respectively. Conclusion Aedes aegypti in Jinghong city and Mengding town have developed different degrees of resistance to pyrethroids and DDT insecticides, but still present high sensitivity to malathion, fenitrothion, propoxur, bendiocarb and fipronil. Measures should be carried out accordingly to limit or stop the use of insecticides to which Ae. aegypti has already developed resistance.
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