目的 对温带臭虫电压门控钠离子通道（vgsc）基因进行测序分析，检测抗药性相关的基因突变，为该地区的臭虫治理提供依据。方法 利用DNA测序技术，获得vgsc基因片段序列，分析击倒抗性相关位点的基因型。结果 与参照品系相应的序列比对，现场采集的6只温带臭虫的vgsc基因序列均存在有义突变，导致编码的电压门控钠离子通道（VGSC）的氨基酸替换，即V419L和L925I突变，突变频率为100%。结论 调查的样品均携带可导致击倒抗性的VGSC在419和925两个位点的突变，表明该温带臭虫种群对拟除虫菊酯类杀虫剂具有较高水平抗性。
Objective To analyze genetic mutations, predict the level of resistance to pyrethroid insecticides in the bedbug Cimex lectularius and provide a guide for bed bug control in this region. Methods Partial sequences of vgsc gene that encodes the target of pyrethroid insecticides (voltage gated sodium channel) were obtained by DNA sequencing, and the knockdown resistance-related mutations were examined. Results The six bed bugs collected on site were found to have genetic mutations at codons 419 and 925, leading to double amino acid substitutions (V419L and L925I) with a frequency of 100%. Conclusion All the investigated bed bugs carry kdr mutations at positions 419 and 925 of the voltage gated sodium channel, predicting that this bed bug population has a higher level of resistance to pyrethroid insecticides.
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