目的 研究盐酸环丙沙星对家蝇幼虫和蛹体内能量物质含量的影响。方法 2013年6-9月，采用实验室家蝇种群作为试虫，幼虫孵化后，提供常规饲料作为对照组，含0.1%、0.3%和0.5%盐酸环丙沙星的饲料作为实验组。测定能量物质采样时，1龄幼虫采用直接研磨法取样，2~3龄幼虫和蛹提取血淋巴作为样品。分别采用考马斯亮蓝法测定蛋白质含量，蒽酮-浓硫酸比色法测定总糖含量，磷酸香草醛显色反应法测定脂肪含量。SPSS 20.0软件用于统计学分析，组间比较采用单因素方差分析，独立样本两两比较采用t检验。结果 0.5%盐酸环丙沙星组幼虫和蛹体内蛋白质含量最高可下降为对照组的4.00%和10.00%；0.1%、0.3%和0.5%组幼虫体内脂肪含量下降约为对照组的75.00%、65.00%和50.00%，蛹体内脂肪含量下降约为对照组的85.00%、75.00%和50.00%；总糖含量均下降约为对照组的80.00%、60.00%和30.00%。同时，喂食盐酸环丙沙星后，家蝇幼虫和蛹体内热量值均随盐酸环丙沙星喂食浓度的升高而下降（F=12.551，P<0.001）。其中，对照组幼虫和蛹体内的热量值高于0.1%、0.3%和0.5%组（t=13.093、11.294、8.601，均P<0.001）；0.1%组体内的热量值高于0.3%和0.5%组（t=10.290、7.842，均P<0.001）；0.3%组体内的热量值高于0.5%组（t=6.919，P<0.001）。结论 盐酸环丙沙星可降低家蝇幼虫和蛹体内蛋白质、脂肪和总糖等能量物质的合成，降低其体内的热量值，从而影响家蝇个体发育和种群繁衍。
Objective To investigate the effect of ciprofloxacin hydrochloride on the content of energy substances in the larvae and pupae of Musca domestica. Methods From June to September, 2013, the laboratory population of M. domestica was used for the experiment. The newly hatched larvae were divided into control group (fed with normal forage) and three experimental groups (treated with forage containing 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride, respectively). The grinding method was used for the sampling of energy substances in the 1st instar larvae, and hemolymph was collected from the 2nd and 3rd instar larvae and pupae. The Coomassie brilliant blue method was used to measure the content of proteins, anthrone-sulfuric acid colorimetry was used to measure the content of total sugars, and phosphoric acid-vanillin chromogenic reaction was used to measure the content of lipids. SPSS 20.0 software was used for statistical analysis; a one-way analysis of variance was used for comparison between groups; the t-test was used for comparison between two groups. Results The content of proteins in the larvae and pupae in the 0.5% ciprofloxacin hydrochloride group decreased to a maximum of 4.00% and 10.00% of that in the control group; the content of lipids in the larvae in the 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride groups decreased to 75.00%, 65.00%, and 50.00% of that in the control group, while the content of lipids in the pupae in the 0.1%, 0.3%, and 0.5% groups decreased to 85.00%, 75.00%, and 50.00% of that in the control group; the content of total sugars in the 0.1%, 0.3%, and 0.5% groups decreased to 80%, 60%, and 30% of that in the control group. After the larvae and pupae of M. domestica were fed with ciprofloxacin hydrochloride, their caloric values decreased with the increase in the concentration of ciprofloxacin hydrochloride (F=12.551, P<0.001). The control group had higher caloric values of the larvae and pupae than the 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride groups (t=13.093, 11.294, 8.601, all P<0.001); the 0.1% group had higher caloric values than the 0.3% and 0.5% ciprofloxacin hydrochloride groups (t=10.290, 7.842, all P<0.001); the 0.3% group had higher caloric values than the 0.5% group (t=6.919, P<0.001). Conclusion Ciprofloxacin hydrochloride can reduce the synthesis of energy substances (proteins, lipids, and total sugars) in the larvae and pupae of M. domestica and thus reduce their caloric values, thus effects the individual development and population multiplication of M. domestica.
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