目的 调查贵州省不同地区蚊虫种类及蚊媒病毒的分布情况，为蚊媒传染病的防治提供依据。方法 2018年8月在贵州省黎平、从江县、花溪、乌当区4个采集点使用诱蚊灯法采集蚊虫。通过分子生物学方法鉴定病毒种类，并利用生物信息学软件对新分离病毒的序列进行分析。结果 在贵州省上述4个采集点共采集蚊虫3属4种17 018只，包括三带喙库蚊（34.0%）、淡色库蚊（2.3%）、中华按蚊（24.7%）和骚扰阿蚊（39.0%），其中骚扰阿蚊数量最多。从蚊虫标本中共分离到2株病毒，经分子生物学鉴定，从三带喙库蚊中分离的病毒株GZWK属于基因Ⅰ型流行性乙型脑炎病毒（JEV），从骚扰阿蚊中分离的病毒株GZWA为基因Ⅲ型JEV。结论在贵州省调查的不同地区中骚扰阿蚊为优势蚊种，并在蚊虫中分离到JEV。GZWK与基因Ⅰ型JEV VN105越南分离株进化关系较近，GZWA与基因Ⅲ型JEV FJ0339福建省分离株进化关系较近。
薛志静, 王君, 宋秀平, 栗冬梅, 赵宁, 闫冬明, 梁文琴, 周敬祝, 王丹, 张瑞玲, 张忠, 刘起勇
. 贵州省不同地区2018年蚊虫及蚊媒病毒调查[J]. 中国媒介生物学及控制杂志, 2019
, 30(3)
: 259
-263
.
DOI: 10.11853/j.issn.1003.8280.2019.03.007
Objective To investigate the distribution of mosquito species and mosquito-borne viruses in different regions of Guizhou province, China, and to provide evidence for the prevention and control of mosquito-borne diseases. Methods Mosquito samples were collected using mosquito lamps in Liping, Congjiang county, Huaxi, and Wudang district in Guizhou province in August 2018. Virus species were identified by molecular biology methods and the sequences of newly isolated viruses were analyzed using bioinformatics software. Results A total of 17 018 mosquitoes belonging to 3 genera and 4 species were collected from the above four sites in Guizhou province, including Culex tritaeniorhynchus (34.0%), Cx. pipiens pallens (2.3%), Anopheles sinensis (24.7%), and Armigeres subalbatus (39.0%). Among these species, Ar. subalbatus was the most collected one. Two strains of viruses were isolated from mosquito specimens and identified by molecular biology. The virus strain GZWK isolated from Cx. tritaeniorhynchus belonged to genotype I Japanese encephalitis virus (JEV), and the virus strain GZWA isolated from Ar. subalbatus belonged to genotype Ⅲ JEV. Conclusion Armigeres subalbatus was the dominant species in the regions investigated in Guizhou, and the Japanese encephalitis virus was also isolated in the study. The GZWK strain has a close phylogenetic relationship with the genotype I JEV VN105 strain from Vietnam, and the GZWA strain has a close phylogenetic relationship with the genotype Ⅲ JEV FJ0339 strain from Fujian province, China.
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