目的 比较双层叠帐法和BG-trap诱捕器法2种媒介伊蚊监测方法在登革热疫点的捕蚊效果，为科学开展监测提供参考。方法 2017年9－10月，选择杭州市12个区（县）的160个住宅区，包括核心区、警戒区及监控区，同时使用双层叠帐法和BG-trap诱捕器法对居民区及其周围环境进行媒介伊蚊应急监测，2种方法设置间隔100 m以上，监测30 min，分别计算叮咬指数（只/h）和诱蚊指数（只/h）。采用单因素方差分析对2种诱蚊方法在不同区域类型的应用进行统计学分析，应用配对样本t检验对2种诱蚊方法进行比较分析。结果 双层叠帐法和BG-trap诱捕器法均能捕获白纹伊蚊，BG-trap诱捕器法捕获白纹伊蚊903只，双层叠帐法捕获456只。BG-trap诱蚊器法在不同区域的捕获率差异有统计学意义（F=9.951，P<0.05），核心区 < 警戒区 < 监控区；双层叠帐法在不同区域的捕获率不同，但差异无统计学意义（F=2.677，P=0.072）。分别对核心区、警戒区和所有区域总和进行2种方法配对t检验，BG-trap诱蚊器法的捕获率高于双层叠帐法，2种方法捕获白纹伊蚊的差异有统计学意义（t=2.786、2.351、3.976，均P<0.05）。结论 2种监测方法均可用于媒介伊蚊监测，双层叠帐法监测适合基层常规监测成蚊密度，而BG-trap诱蚊器法更加适合登革热疫情等蚊媒传染病的应急监测使用。
Objective To compare the effectiveness of two vector surveillance methods, double mosquito net and BG-mosquito trap (BG-trap), for collecting mosquitoes on the spot of dengue fever, and to provide a basis for rational surveillance approaches. Methods From September to October, 2017, 160 residential areas were selected from 12 districts and counties of Hangzhou, Zhejiang province, China, which contained the core area, warning area, and surveillance area. The double mosquito net and BG-trap were both used for emergency surveillance of vector Aedes albopictus in residential areas and the surrounding environments. The interval between the two traps was longer than 100 m. The surveillance lasted for 30 min. The biting index (mosquitoes/hour) and mosquito index (mosquitoes/h) were calculated. One-way analysis of variance was used to analyze two mosquito trapping methods in different areas. The paired t test was used to compare the efficiency between the two methods. Results Both the double mosquito net and BG-trap were able to capture Ae. albopictus, 903 mosquitoes were captured by BG-trap trap and 456 by the double mosquito net method. BG-trap had the highest efficiency in the monitoring area, followed by the warning area and the core area (F=9.951, P<0.05). There was no significant difference in the efficiency of the double mosquito net between different areas (F=2.677, P=0.072). According to the results of paired t test, BG-trap had significantly higher efficiency than the double mosquito net in the core area, warning area, and all areas (t=2.786, 2.351, 3.976, all P<0.05). Conclusion Both surveillance methods can be used to monitor the vector mosquito. The double mosquito net method is suitable for routine adult mosquito density surveillance, while BG-trap is more suitable for the emergency surveillance of mosquito-borne infectious diseases such as dengue fever.
[1] 中国疾病预防控制中心. 登革热媒介伊蚊监测指南中国疾病预防控制中心关于印发登革热防治技术指南的通知(中疾控传防发[2014] 360号),附件3[S].中国疾病预防控制中心,2004.
[2] Maciel-de-Freitas R,Eiras AE,Lourenço-de-Oliveira R. Field evaluation of effectiveness of the BG-Sentinel,a new trap for capturing adult Aedes aegypti (Diptera:Culicidae)[J]. Mem Inst Oswaldo Cruz,2006,101(3):321-325. DOI:10.1590/S0074-02762006000300017.
[3] Kröckel U,Rose A,Eiras AE,et al. New tools for surveillance of adult yellow fever mosquitoes:comparison of trap catches with human landing rates in an urban environment[J]. J Am Mosq Control Assoc,2006,22(2):229-238. DOI:10.2987/8756-971X(2006)22[229:NTFSOA]2.0.CO;2.
[4] Gama RA,da Silva IM,Eiras M,et al. Development of the BG-Malaria trap as an alternative to human-landing catches for the capture of Anopheles darling[J]. Mem Inst Oswaldo Cruz,2013,108(6):763-771. DOI:10.1590/0074-0276108062013013.
[5] Tangena JAA,Thammavong P,Hiscox A,et al. The human-baited double net trap:an alternative to human landing catches for collecting outdoor biting mosquitoes in Lao PDR[J]. PLoS One,2015,10(9):e0138735. DOI:10.1371/journal.pone.0138735.
[6] Li YJ,Su XH,Zhou GF,et al. Comparative evaluation of the efficiency of the BG-Sentinel trap,CDC light trap and Mosquito-oviposition trap for the surveillance of vector mosquitoes[J]. Parasit Vect,2016,9:446. DOI:10.1186/s13071-016-1724-x.
[7] 王学军,赵志刚,刘峰,等. 两种蚊虫密度监测方法实验室及现场诱蚊效果观察[J]. 中国媒介生物学及控制杂志,2011,22(6):590-591,594.
[8] Owino EA,Sang R,Sole CL,et al. An improved odor bait for monitoring populations of Aedes aegypti-vectors of dengue and chikungunya viruses in Kenya[J]. Parasit Vect,2015,8:253. DOI:10.1186/s13071-015-0866-6.
[9] 郭玉红,陈传伟,朱丽,等. 河南省永城市白纹伊蚊日活动节律初步研究[J]. 中国媒介生物学及控制杂志,2016,27(5):474-486. DOI:10.11853/j.issn.1003.8280.2016.05.016.
[10] 中国疾病预防控制中心. 登革热媒介伊蚊控制指南中国疾病预防控制中心关于印发登革热防治技术指南的通知(中疾控传防发[2014] 360号),附件4[S].中国疾病预防控制中心,2014.
[11] 郭玉红,刘京利,鲁亮,等. 诱蚊灯法与人工小时法捕蚊效果比较研究[J]. 中国媒介生物学及控制杂志,2012,23(6):529-532.
