目的 监测河南省开封、安阳市淡色库蚊种群对氯菊酯、溴氰菊酯、高效氯氰菊酯、仲丁威和双硫磷等常用杀虫剂的抗药性水平，分析其变化趋势，为化学防治提供科学合理的用药指导。方法 于2012、2014和2016年的6-8月，在开封和安阳市区的苗圃肥水池、废弃积水池等蚊虫孳生场所采集淡色库蚊卵块，带回实验室饲养后取子1代4龄幼虫，采用国家标准敏感基线幼虫浸渍法进行抗性测定。结果 2012、2014和2016年淡色库蚊开封种群对氯菊酯的抗性从低抗发展为高抗，半数致死浓度（LC₅₀）为0.147 5~0.523 8 mg/L，抗性倍数为5.76~20.46倍；对溴氰菊酯的抗性为高抗，LC₅₀为0.006 2~0.011 7 mg/L，抗性倍数为31.00~58.50倍；对高效氯氰菊酯、仲丁威和双硫磷的抗性维持在敏感或低抗，LC₅₀为0.001 6~0.513 4 mg/L，抗性倍数为0.22~7.90倍。淡色库蚊安阳种群对氯菊酯、高效氯氰菊酯、仲丁威和双硫磷的抗性维持在敏感或低抗，LC₅₀为0.000 8~0.915 5 mg/L，抗性倍数为0.50~8.00倍；对溴氰菊酯的抗性为中抗或高抗，LC₅₀为0.002 2~0.012 2 mg/L，抗性倍数为11.00~61.00倍。结论 淡色库蚊开封及安阳种群对拟除虫菊酯类和有机磷类杀虫剂产生了不同程度的抗药性，未对氨基甲酸酯类杀虫剂产生抗性。两地在今后的蚊虫防制中，应坚持以孳生地治理为主的综合防制措施，科学有效的使用化学杀虫剂。

Objective Survey the resistance trend of Culex pipiens pallens to Permethrin, Deltamethrin, beta-Cypermethrin, Fenobucarb, and Temephos in Kaifeng and Anyang, to provide scientific guidance for chemical prevention. Methods Larvae were collected from flower gardens or abandoned pools and so on in the urban area of Kaifeng and Anyang from June to August in 2012, 2014, and 2016. Results The median lethal concentration (LC₅₀) of the Kaifeng population against Permethrin in 2012, 2014, 2016 were 0.147 5-0.523 8 mg/L, the resistance ratios were 5.76-20.46 fold; against Deltamethrin were 0.006 2-0.011 7 mg/L, the resistance ratios were 31.00-58.50 fold; against beta-Cypermethrin, Fenobucarb and Temephos were 0.001 6-0.513 4 mg/L, the resistance ratios were 0.22-7.90 fold. The LC₅₀ of the Anyang population against Permethrin, beta-Cypermethrin, Fenobucarb and Temephos were 0.000 8-0.915 5 mg/L, the resistance ratios were 0.50-8.00 fold; against Deltamethrin were 0.002 2-0.012 2 mg/L, the resistance ratios were 11.00-61.00 fold. Conclusion Culex pipiens pallens in Kaifeng and Anyang showed different levels of resistance to pyrethroids and organophosphorus insecticides, but still susceptible to carbamate insecticides. The strategy of mosquito control in Kaifeng and Anyang should focus on source reduction. Chemical control should be limited, scientific and effective.