目的 了解衢州市开化县鼠类伯氏疏螺旋体的感染情况。方法 2015-2016年每年的4-11月在开化县选择不同生境，采用鼠笼捕鼠，采集鼠脾标本，巢式PCR用于扩增伯氏疏螺旋体5S~23S rRNA间隔区片段，并对阳性片段进行测序。结果 149份样本中检出阳性10份，总阳性率为6.71%，阳性鼠种有白腹巨鼠、北社鼠、针毛鼠、黄胸鼠、黑线姬鼠和褐家鼠，其中优势鼠种为黑线姬鼠、针毛鼠和北社鼠，阳性率分别为4.05%、10.00%和10.00%。测序结果表明，针毛鼠、北社鼠中伯氏疏螺旋体为B.burgdorferi sensu stricto，褐家鼠中伯氏疏螺旋体为B.valaisiana。结论 开化县北社鼠和针毛鼠可能是莱姆病的重要宿主，鼠类存在2种不同基因型的伯氏疏螺旋体感染，应继续加强野生动物中伯氏疏螺旋体的监测，同时密切关注人群中莱姆病的发病状况。
Objective The present study was conducted to investigate the infection of Borrelia burgdorferi in rodents in Kaihua county. Methods Nested PCR were used to amplify 5S-23S rRNA spacer fragments of B. burgdorferi from kidney samples of rodents captured by mouse cage in Kaihua county. And the positive samples were sequenced and analyzed. Results A total of 10 rodents were positive in 149 samples tested,with the prevalence of 6.71%. The positive samples were identified from Rattus edwardsi, Niviventer niviventer, N. fulvescens, R. tanezumi, Apodemus agrarius, and R. norvegicus. The predominant species in Kaihua county were Ap. agrarius, R. fulvescens, and N. niviventer and the positive rates were 4.05%, 10.00%, and 10.00%, respectively. The spleen specimens from the N. confucianus and N. fulvescens were positive for B.burgdorferi sensu stricto, and that from R. norvegicus was positive for B.valaisiana. Conclusion The N. confucianus and N. fulvescens are considered as important reservoirs. The infection of two B. burgdorferi genotypes in rodents was confirmed in Kaihua county. It is very important to enhance the B. burgdorferi surveillance in wild animals and focus the morbidity in population.
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