目的 准确快速鉴定居室内检获的螨类。方法 对2016年7月于芜湖市某儿童房内毛绒玩具上检获的大部分螨类进行形态鉴定，对6只螨扩增线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因，基于该基因以邻接法构建已知美绥螨科螨类的系统进化树。结果 经形态鉴定，检获螨类为雌性羽美绥螨（Ameroseius plumosus），属革螨股、囊螨总科、美绥螨科。首次扩增了羽美绥螨COⅠ基因的DNA条形码，6只个体间的DNA条形码差异<0.5%，判断这6只个体为同一种类。系统进化树显示羽美绥螨与美绥螨科的种类聚在一支。结论 首次在毛绒玩具中发现羽美绥螨。应用形态鉴定和DNA条形码相结合的鉴定方法，可有效提高医学媒介生物种类鉴别能力和准确度。
王逸枭, 郑凌霄, 管国宇, 尹灿灿, 刘啸, 汪书然, 温子昂, 陈樱玮, 程序, 孙恩涛
. 居室内检获的羽美绥螨形态和DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2018
, 29(6)
: 582
-585
.
DOI: 10.11853/j.issn.1003.8280.2018.06.007
Objective To identify the mites accurately and rapidly. Methods Most of the mites seized on a plush toy in a Children's room in Wuhu in July 2016 were identified by morphology. Six individuals were amplified by the cytochrome C oxidase subunit I (COⅠ) gene. Meanwhile, based on the gene, phylogenetic tree of the known Ameroseiidae mites was constructed by Neighbor-Joining method. Results According to morphological observations, the mite we seized was identified as female Ameroseius plumosus, belonging to Ameroseiidae, Ascoidea, Gamasina. The DNA barcode of the COⅠgene was obtained from the mite for the first time. The six individuals were identified as the same species because all the inter-individual's DNA barcode differences were less than 0.5%. Phylogenetic tree shows that A. plumosus is associated with other individuals of Ameroseiidae. Conclusion It's the first time that the A. plumosus was found on a plush toy. Combining morphology and DNA barcode techniques together could provide a good tool for the identification of the medical vectors rapidly and accurately.
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