目的 了解新疆维吾尔自治区（新疆）乌苏市古尔图地区长尾黄鼠的巴尔通体感染状况，为该地区鼠传疾病风险预警与防控措施制定提供科学依据。方法 2017年5-9月采用一日弓形夹法在查岗果勒、布兰布拉克、白石头等地捕获长尾黄鼠86只，无菌采集鼠体肾脏，试剂盒法提取全基因组DNA。应用实时荧光定量PCR法检测样本DNA巴尔通体特异性基因ssrA，计算感染率，分析样本的检测循环阈值（cp值）。结果 42只长尾黄鼠的巴尔通体实时荧光定量PCR检测为阳性，阳性率为48.84%；阳性样本cp值最低为27.49，最高为37.83，其中88.10%阳性样本的cp值在35.00~37.83之间，查岗果勒、布兰布拉克、白石头三地巴尔通体的阳性率分别为48.48%、60.87%和40.00%；布兰布拉克地区巴尔通体感染率、阳性动物染蚤率、蚤指数均高于查岗果勒和白石头地区；三地巴尔通体感染率与动物染蚤率差异无统计学意义（P>0.05）。结论 新疆乌苏市古尔图地区长尾黄鼠间存在巴尔通体自然感染，传播范围广，但群体带菌量较低。
Objective To understand the infection status of Bartonella in Citellus undulatus from Guertu, and to provide the basis for risk early warning and control measures of rodent-borne diseases. Methods In total, 86 C. undulatus were collected from Chagangguole, Bulanbulake and Baishitou by bow-shaped clip method during 24 hours, the kidneys of the captured rodents were sampled under aseptic condition, genome DNA was extracted and detected by real-time fluorescence quantitative PCR targeting ssrA of Bartonella. The rate of infection were calculated and analyzed by cp test (cp value). Results A total of 86 C. undulatus were tested, the infection rate of Bartonella in those rodents was 48.84% (42/86). The cp of positive samples had the highest value of 37.83 and the lowest value of 27.49, 88.10% of positive samples was in the "35.00-37.83" range. The infection rate of Chagangguole, Bulanlulake and Baishitou were 48.48%, 60.87%, 40.00%, respectively. The flea infection rate and flea index in Bulanlulake were higher than those of other places, the infection rate had no correlation with the flea infection in Guertu (P>0.05). Conclusion There were natural infection of Bartonella among C. undulatus in Guertu, with tendency of wide spread, but pathogen loads were relatively low in wild rodents.
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