目的 调查武汉市不同生境家蝇种群的抗药性现状，为杀虫剂的合理使用和科学防制提供依据。方法 采用微量点滴法测定羽化后3～4日龄雌性家蝇成虫的半数致死量（LD₅₀），并计算抗性倍数，运用Kruskal-Wallis H和Mann-Whitney U秩和检验分析种群间的差异性。结果 居民区、农贸市场和垃圾中转站家蝇种群对高效氯氰菊酯、氯氰菊酯、溴氰菊酯和甲基吡恶磷均产生高抗，LD₅₀在0.095～0.685 μg/只之间，抗性倍数在26.36～710.00倍之间，差异均无统计学意义（χ²=0.007、0.284、1.280、0.152，P=0.996、0.868、0.527、0.927）。居民区和农贸市场家蝇种群对胺菊酯呈低抗水平；垃圾中转站家蝇种群对胺菊酯呈敏感水平，差异有统计学意义（χ²=6.292，P=0.047）。两两种群间比较发现，垃圾中转站家蝇种群对胺菊酯的抗性水平明显低于居民区（U=8.000，P=0.033）和农贸市场家蝇种群（U=7.500，P=0.028）；而居民区和农贸市场家蝇种群间差异无统计学意义（U=24.000，P=0.949）。结论 武汉市3种不同生境家蝇种群对高效氯氰菊酯、氯氰菊酯、溴氰菊酯和甲基吡恶磷均呈较高抗性，对胺菊酯的敏感度有一定差异。建议在今后灭蝇工作中遵循综合防制原则，科学合理地使用杀虫剂，延缓家蝇抗性发展。

Objective To investigate the current insecticide resistance situation of Musca domestica in different habitats in Wuhan, to provide scientific basis for rational application of insecticides and effective control. Methods The micro-drop method was applied to test medium lethal dose (LD₅₀) of 3-4 day old female M. domestica to pesticides. Kruskal-Wallis H Rank Sum Test and Mann-Whitney U Rank Sum Test were used for statistical significance analysis. Results In residential areas, farmers' markets and refuse transfer stations, M. domestica showed high resistance to cypermethrin, deltamethrin and methylpyridine. The LD₅₀ of the M. domestica ranged from 0.095 to 0.685 μg per fly, and resistance ratios were 26.36 to 710.00-fold, there was no significant difference (χ²=0.007, 0.284, 1.280, 0.152, P=0.996, 0.868, 0.527, 0.927). Musca domestica in residential areas and farmers' markets showed low resistance to amethrin, while M. domestica in refuse transfer stations were susceptible to cypermethrin, with statistical significance (χ²=6.292, P=0.047). The results showed that the resistance level of M. domestica to cypermethrin in refuse transfer station was significantly lower than that in residential area (U=8.000, P=0.033) and farmers market (U=7.500, P=0.028), but there was no significant difference between residential area and farmer market (U=24.000, P=0.949). Conclusion The M. domestica strains in three different habitats in Wuhan demonstrated high resistance to beta-cypermethrin, cypermethrin, deltamethrin and azamethiphos, to which the difference among the susceptibility of the M. domestica strains from three different habitats was detected. Therefore, it is suggested to take integrated control measures and apply insecticides scientifically and rationally to avoid the development of pesticide resistance in M. domestica.