目的 研究不同浓度盐酸环丙沙星对家蝇幼虫和蛹体内总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）3种抗氧化酶活性的影响。方法 家蝇幼虫孵化后分别提供常规饲料、含0.1%、0.3%和0.5%盐酸环丙沙星的饲料。每天分别取样，至家蝇羽化。家蝇幼虫采用直接研磨法获得酶液，家蝇蛹先去除蛹壳后再进行研磨获得酶液。分别采用试剂盒测定T-SOD、CAT和POD活力，数据采用多重比较进行统计学分析。结果 对照组、0.1%、0.3%处理组家蝇幼虫和蛹体内T-SOD、CAT和POD活力间差异无统计学意义（P>0.05），而0.5%盐酸环丙沙星饲喂的家蝇幼虫和蛹体内3种抗氧化酶活力均高于前3组，其中喂食0.5%盐酸环丙沙星后家蝇幼虫体内的T-SOD活性最高可达对照组的30倍，蛹体内的T-SOD活性最高可达对照组的40倍；喂食0.5%盐酸环丙沙星后，家蝇幼虫体内CAT活性最高可达对照组的3倍，蛹体内的活性最高可达对照组的6倍；喂食盐酸环丙沙星后，家蝇幼虫体内POD活性最高可达对照组的2倍，而蛹体内的POD活性最高可达对照组的7倍。结论 低浓度盐酸环丙沙星对家蝇幼虫和蛹体内的抗氧化酶活力影响较小，而高浓度盐酸环丙沙星则可导致抗氧化酶活力显著升高。
Objective To investigate the effects of ciprofloxacin on the antioxidases activities of Musca domestica larvae and pupae. Methods T-SOD, CAT, and POD activities were detected and analyzed every day. The housefly larvae being fed with 0.1%, 0.3%, and 0.5% ciprofloxacin, named 0.l% ciprofloxacin group, 0.3% ciprofloxacin group and 0.5% ciprofloxacin group, respectively. Fly larvae fed with normal feed named control group. The detection kits of T-SOD, CAT, and POD were used to detect the enzymatic activity of different groups. The assay on enzymes enzymatic activity of different samples was repeated five times. The statistical methods was used for multiple comparison. Results The enzymatic activities of T-SOD, CAT, and POD were had no significant differences among control groups, 0.1% and 0.3% ciprofloxacin groups. But the enzymatic activities of T-SOD, CAT, and POD at 0.5% ciprofloxacin group were significantly higher than the other three groups. After being fed with 0.5% ciprofloxacin, the T-SOD activity of larvae and pupae were up to 30 and 40 times higher than the control groups, respectively. The activity of CAT in 0.5% ciprofloxacin group of larvae and pupae were up to 3 and 6 times higher than the control groups. In 0.5% ciprofloxacin group, the POD activity of larvae and pupae were up to 2 and 7 times higher than the control groups, respectively. Conclusion Low concentrations of ciprofloxacin had no significant impact on the antioxidase activities of housefly larvae and pupae, high concentration of ciprofloxacin did activate the antioxidase activity.
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