目的 对四川省新龙县分离的鼠疫耶尔森菌（鼠疫菌）开展差异区段（DFR）分型研究，为鼠疫研究提供科学依据。方法 选取新龙县和其他5个鼠疫县分离的鼠疫菌16株，按鼠疫菌DFR分型方法，使用23个DFR和pMT1的扩增引物，对被试菌株DNA进行PCR扩增，采用BioNumerics 6.6软件进行分型，确定新龙县鼠疫菌DFR基因型。结果 石渠县鼠疫菌菌株聚为第1类，为Genomovar14型；新龙县鼠疫菌DFR基因型与德格、巴塘、理塘、雅江县相同，聚为第2类，均为Genomovar05型。结论 新龙县分离到的鼠疫菌基因型为Genomovar05型，新龙县鼠疫自然疫源地属于青藏高原喜马拉雅旱獭鼠疫自然疫源地的一部分。
Objective We studied the DFR (different region)-type data of Yersinia pestis in Xinlong county and provided a scientific basis for prevention and treatment of plague. Methods One strain of Y. pestis isolated from plague foci in Xinlong county compared with 16 strains of Y. pestis isolated from other plague foci in Ganzi Tibeian Autonomous Prefecture, and genotyped by primers of 23 DFRs and PMT1. To confirm the genotype of strains from Xinlong county, we analyzed the data by BioNumerics 6.6 software. Results The DFR-type of Y. pestis in Xinlong county was same as DFR-type of other counties, all belonging to Genomovar05 type, but different from DFR-type of Shiqu county strains. Conclusion Genotype of Y. pestis isolated from Xinlong county is Genomovar05 type, and Xinlong plague natural foci is the part of Marmota himalayana plague natural foci of Tibetan Plateau.
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