目的 研究绍兴市家蝇雌成虫对残杀威、DDVP、高效氯氰菊酯、氯菊酯、溴氰菊酯5种常用杀虫剂的抗药性，为家蝇的科学防控提供依据。方法 参照WHO推荐的微量点滴法，2017年8－9月于绍兴市主城区捕获家蝇，繁殖1代，测定其羽化后4～5日龄的雌成虫对5种常用杀虫剂的抗药性，计算半数致死量（LD₅₀）及其95% CI。结果 绍兴市家蝇对残杀威、DDVP、高效氯氰菊酯、氯菊酯、溴氰菊酯产生了不同程度的抗药性，其中残杀威已基本失效，无法得出检测结果；对溴氰菊酯、DDVP、高效氯氰菊酯、氯菊酯的LD₅₀分别为0.064 64、3.117 46、0.231 42和0.180 47 μg/♀；抗性倍数分别为71.82、68.82、64.28和16.71倍。结论 绍兴市家蝇对残杀威、DDVP、高效氯氰菊酯、溴氰菊酯的抗性为高抗，对氯菊酯抗药性为中抗；应继续开展病媒生物抗药性监测，科学合理使用杀虫剂。

Objective To investigate the resistance of Musca domestica to commonly used insecticides in Shaoxing city, Zhejiang province, provide guidance in the control of M. domestica. Methods Musca domestica were collected from the main district of Shaoxing during August-September 2017. WHO recommended topical application method was used to determine the resistance levels of M. domestica, which were the age of 4-5 d after eclosion, to five insecticides (propoxur, DDVP, β-cypermethrin, permethrin, deltamethrin). Results Musca domestica in Shaoxing city showed different levels of resistance to the five insecticides. Statistics revealed that the resistance to propoxur could not obtain results by using topical application method. The median lethal dose (LD₅₀) of the adult flies against deltamethrin, DDVP, β-cypermethrin and permethrin were 0.064 64, 3.117 46, 0.231 42, and 0.180 47 μg/♀, and the resistance ratios were 71.82, 68.82, 64.28, and 16.71-fold, respectively; the resistance to deltamethrin was the highest. Conclusion Musca domestica in Shaoxing city have developed resistance to five insecticides in different levels. Insecticides resistance surveillance should be highlighted for rational use of insecticides.