目的 了解湖北省不同地区德国小蠊对常用杀虫剂的抗药性，为科学、合理用药提供理论依据。方法 2016年分别在湖北省武汉、咸宁、黄石、荆州、宜昌、荆门、襄阳、十堰、鄂州和仙桃市采集德国小蠊，带回实验室饲养；采用WHO推荐的药膜法，测定经实验室饲养1代的试虫对溴氰菊酯、高效氯氰菊酯、DDVP、残杀威和乙酰甲胺磷5种常用杀虫剂的半数击倒时间及其95%CI、击倒率和抗性倍数，评价其抗药性情况。结果 武汉、宜昌和黄石市德国小蠊野外种群对溴氰菊酯的抗性倍数较高，分别为8.16、7.71和6.48倍；荆门、武汉和宜昌市德国小蠊野外种群对高效氯氰菊酯的抗性程度较高，分别为19.75、7.06和6.75倍；武汉、十堰和荆门市德国小蠊野外种群对DDVP的抗性倍数较高，分别为61.22、9.13和8.35倍；襄阳市德国小蠊野外种群对残杀威的抗性最高，为6.71倍，其他地区均较低；荆州市德国小蠊野外种群对乙酰甲胺磷的抗性最高，为4.88倍，其他地区均较低。结论 湖北省大部分城市德国小蠊野外种群对DDVP、高效氯氰菊酯和溴氰菊酯的抗性问题非常严重；建议尽量采用环境治理方法，清除蜚蠊赖以栖息的生存条件，尽量减少滞留喷洒。

Objective To investigate the resistance of Blattella germanica to commonly used insecticides,and to provide guidance in B. germanica control. Methods The B. germanica was collected in Wuhan, Xianning, Huangshi, Jingzhou, Yichang, Jingmen, Xiangyang, Shiyan, Ezhou, and Xiantao cities of Hubei province. Collections were maintained in insectary for further processing. The B. germanica used in this study were the first filial generation(F1). The insecticide film method recommended by the World Health Organization was used to determine the median knockdown time(KT50), the corresponding 95%CI, knock-down rate and the levels of resistance of B. germanica exposed to the five commonly used insecticides. Results The resistance ratios to deltamethrin were high in Wuhan, Yichang, and Huangshi(8.16, 7.71, and 6.48 fold). The resistance also reached the high level to beta-cypermethrin in Jingmen, Wuhan, and Yichang(19.75, 7.06, and 6.75 fold). The resistance ratios to dichlorvos were high in Wuhan, Shiyan, and Jingmen(61.22, 9.13, and 8.35 fold). For propoxur and orthene, the resistance ratios were 6.71 and 4.88 in Xiangyang and Jingzhou city, respectively. Conclusion Field collected strains of B. germanica in Hubei province have already developed different resistance levels to dichlorvos, beta-cypermethrin and deltamethrin. Use of environmental management to eliminate the habitat of cockroaches to minimize the residual spray was proposed.