目的 调查江苏各口岸常见蚊虫中沃尔巴克氏体（Wolbachia）的感染情况及其系统发育关系，为基于Wolbachia的防控策略奠定基础。方法 2015年6-9月采用诱蚊灯法和二氧化碳诱捕法在江苏省苏州、常州、无锡、南京、南通、扬州、泰州、连云港、徐州和淮安10个口岸地区诱捕蚊虫，提取单只蚊虫的DNA，PCR扩增后检测蚊虫Wolbachia wsp基因，计算感染率；并对所有wsp基因进行测序及系统发育分析。结果 共检测江苏省10个口岸地区的5种常见蚊虫，Wolbachia总感染率为26.80%，以尖音库蚊复合组感染率（47.81%）最高，其次为骚扰阿蚊（46.24%）、白纹伊蚊（34.06%）和三带喙库蚊（1.25%），而中华按蚊未见感染。这些蚊虫的Wolbachia wsp基因共享10个单倍型，其遗传距离在0.002~0.263之间。Hap-1、Hap-7、Hap-8、Hap-9和Hap-10为同种蚊虫所独有，而Hap-2、Hap-4和Hap-5在江苏不同口岸蚊虫中出现的频率较高，在2~3种蚊虫间共享。其中Hap-1和Hap-2属A大组Wolbachia，而其余单倍型属B大组Wolbachia。结论 江苏各口岸常见蚊虫共携带2种Wolbachia，其在蚊虫种内、种间分布多样，基本掌握了江苏不同口岸蚊虫Wolbachia的感染情况，是将来基于Wolbachia特性对蚊媒及蚊媒病控制的关键。
Objective To investigate the infection status of Wolbachia and its phylogenetic relationships among common mosquitoes at Jiangsu ports, and to lay the foundation for mosquito control strategy based on Wolbachia. Methods During June to September in 2015, the light trap and carbon dioxide trap were used in 10 areas of Jiangsu ports(Suzhou, Changzhou, Wuxi, Nanjing, Nantong, Yangzhou, Taizhou, Lianyungang, Xuzhou, and Huaian), and DNA was extracted from the trapped mosquitoes, then Wolbachia wsp gene was amplified by PCR. All wsp genes were sequenced and phylogenetic analysis followed. Results We analyzed 5 species of common mosquitoes in Jiangsu ports, the total infection rate of Wolbachia was 26.80%, and the Culex pipiens complex infection rate(47.81%) was the highest, followed by Armigeres subalbatus(46.24%), Aedes albopictus(34.06%), and Cx. tritaeniorbynchus(1.25%). Anopheles sinensis were not infected. The mosquito Wolbachia wsp gene shared 10 haplotypes, and genetic distances between these haplotypes were 0.002-0.263, Hap-1 Hap-7, Hap-8, Hap-9, and Hap-10 were unique for the same mosquito species, while Hap-2, Hap-4, and Hap-5 in Jiangsu port mosquitoes appeared at high frequency, being shared in the 2-3 species. Among them, Hap-1 and Hap-2 belong to A group, while the rest belong to B group. Conclusion The common mosquitoes of Jiangsu ports have carried 2 species of Wolbachia, its intraspecific and interspecific distribution was highly variable. Through this investigation, the infection of Wolbachia at Jiangsu ports is preliminarily understood, the survey will serve the key of biological control based on the characteristics of Wolbachia.
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