淡色库蚊抗性种群和敏感品系中铁蛋白基因表达量的分析

陆靖, 史琦琪, 程鹏, 公茂庆, 崔文, 谭文彬

doi:10.11853/j.issn.1003.8280.2018.02.005

中国媒介生物学及控制杂志 >

2018 , Vol. 29 >Issue 2: 134 - 137

DOI: https://doi.org/10.11853/j.issn.1003.8280.2018.02.005

论著

淡色库蚊抗性种群和敏感品系中铁蛋白基因表达量的分析

展开

1 济南大学, 山东省医学科学院医学与生命科学学院, 济南 250001;
2 山东省寄生虫病防治研究所, 山东济宁 272033;
3 济宁医学院司法鉴定中心, 山东济宁 272067;
4 济宁医学院基础医学院, 山东济宁 272067

陆靖,女,在读硕士,主要从事病原生物学研究,Email:18766888418@163.com

收稿日期: 2017-12-26

网络出版日期: 2018-04-20

基金资助

国家自然科学基金（81271876，81471985，81672059）；山东省自然科学基金（ZR2015YL023）；山东省高等学校科技计划项目（J13LK12）；山东省高等学校青年骨干教师国内访问学者项目；山东省高校优秀科研创新团队计划；济宁市科技助推新旧动能转换计划项目（2017SMNS001）

收起

Comparative analysis on expression levels of the ferritin gene in Culex pipiens pallens

Expand

1 School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250001, Shandong Province, China;
2 Shandong Institute of Parasitic Diseases;
3 Center of Forensic Science of Jining Medical University;
4 Academy of Basic Medicine, Jining Medical University

Received date: 2017-12-26

Online published: 2018-04-20

Supported by

Supported by the National Natural Science Foundation of China(No. 81271876,81471985,81672059), the Natural Science Foundation of Shandong Province(No. ZR2015YL023), Shandong Province Higher Educational Science and Technology Program(No. J13LK12), Program of Domestic Study for Young Scholar of University in Shandong Province Sponsored by Shandong Provincial Educational Department, Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province, and the Program of Science and Technology Boosting for New and Old Kinetic Energy Conversion Plan in Jining City(No. 2017SMNS001)

Fold

摘要

目的对淡色库蚊抗性种群和敏感品系中的铁蛋白基因表达量进行分析。方法设计引物，扩增目的基因全长、纯化测序、序列分析，荧光定量PCR检测铁蛋白基因的表达水平；采用假设检验中的t检验方法，对2种不同处理的淡色库蚊样本体内铁蛋白基因表达量进行统计分析。结果成功扩增淡色库蚊铁蛋白基因片段，经荧光定量PCR测定，淡色库蚊敏感品系铁蛋白荧光定量（F_敏）和抗性种群铁蛋白荧光定量（F_抗）平均值分别为11.41和15.73，内参基因荧光定量（β_敏和β_抗）平均值分别为10.03和18.27，铁蛋白基因在淡色库蚊抗性种群中的表达量是敏感品系的15.08倍，t检验结果显示，两样本体内铁蛋白基因表达差异有统计学意义（t=26.100，P < 0.001）。结论淡色库蚊铁蛋白基因可作为蚊虫抗性检测及治理的靶标基因。

关键词： 淡色库蚊; 铁蛋白; 荧光定量聚合酶链式反应

本文引用格式

陆靖, 史琦琪, 程鹏, 公茂庆, 崔文, 谭文彬 . 淡色库蚊抗性种群和敏感品系中铁蛋白基因表达量的分析[J]. 中国媒介生物学及控制杂志, 2018 , 29(2) : 134 -137 . DOI: 10.11853/j.issn.1003.8280.2018.02.005

Abstract

Objective To analyze the ferritin gene expression levels in different strains of Culex pipiens pallens. Methods The primers were designed and synthesized, target gene was amplified by fluorescent quantitative PCR to detect the expression level between resistant strain and susceptible strains of Cx. pipiens pallens. A t test method was used to detect the statistical significance for the two samples. Results The ferritin gene of Cx. pipiens pallens was cloned successfully. The quantitative average of ferritin fluorescence was 11.41 in susceptible strain and 15.73 in resistant strain; the quantitative average of reference gene fluorescence was 10.03 in susceptible strain, and 18.27 in resistant strain. Fluorescent quantitative PCR proved the ferritin gene expression level of resistant strain was 15.08 times that of susceptible strain, t test showed that two samples of ferritin gene expression differences were statistically significant(t=26.100, P < 0.001). Conclusion Culex pipiens pallens ferritin gene can be used as a new gene target for resistant detection and management.

Key words： Culex pipiens pallens; Ferritin; Fluorescent quantitative polymerase chain reaction

参考文献

[1] Rivero A,Vézilier J,Weill M,et al. Insecticide control of vector-borne diseases:when is insecticide resistance a problem?[J]. PLoS Pathog,2010,6(8):e1001000.
[2] Nauen R. Insecticide resistance in disease vectors of public health importance[J]. Pest Manag Sci,2007,63(7):628-633.
[3] 谭文彬,王霄,袁常秀,等. 三种品系白纹伊蚊IRE-BP1基因表达差异的比较[J]. 国外医学医学地理分册,2012,33(4):230-232.
[4] 谭文彬,王霄,袁常秀,等. IRE-BP1基因在三种淡色库蚊体内表达量的比较分析[J]. 中国热带医学,2012,12(4):421-422,426.
[5] 王霄,杜彦,杨磊,等. 中华按蚊IRE-BP1基因的表达与氯氰菊酯抗性的相关性初探[J]. 中国热带医学,2015,15(9):1037-1039.
[6] Tan WB,Wang X,Cheng P,et al. Cloning and overexpression of transferrin gene from cypermethrin-resistant Culex pipiens pallens[J]. Parasitol Res,2012,110(2):939-959.
[7] 谭文彬,王霄,程鹏,等. 中华按蚊不同株转铁蛋白基因表达水平的比较研究(英文)[J]. 济宁医学院学报,2013,36(4):234-238.
[8] 王霄,谭文彬,程鹏,等. 三种品系白纹伊蚊转铁蛋白基因表达量的比较分析[J]. 国外医学医学地理分册,2011,32(3):182-183,187.
[9] Reif DW. Ferritin as a source of iron for oxidative damage[J]. Free Radic Biol Med,1992,12(5):417-427.
[10] Torti FM,Torti SV. Regulation of ferritin genes and protein[J]. Blood,2002,99(10):3505-3516.
[11] Thomson AM,Rogers JT,Leedman PJ. Iron-regulatory proteins, iron-responsive elements and ferritin mRNA translation[J]. Int J Biochem Cell Biol,1999,31(10):1139-1152.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献